| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,615 | G→T | 14.0% | T276T (ACC→ACA) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,615 | 0 | G | T | 14.0% | 398.3 / 29.8 | 130 | T276T (ACC→ACA) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (57/54); new base T (13/5); total (70/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01 | |||||||||||
Only 100 of 135 total aligned reads displayed.
ACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGC > minE/1933546‑1933684 | aCAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAAc > 1:1482309/1‑71 (MQ=255) cAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAAct > 1:3825783/1‑71 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct < 1:1261435/71‑1 (MQ=255) gATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTgg > 1:3818034/1‑71 (MQ=255) tCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGt > 1:831731/1‑70 (MQ=255) cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTgg > 1:909813/1‑67 (MQ=255) gTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATg < 1:2209943/71‑1 (MQ=255) cTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTAt < 1:1580681/65‑1 (MQ=255) tCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTg < 1:1292786/51‑1 (MQ=255) gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGG‑TATGACGTACGGa < 1:1870443/71‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACTTACGGa < 1:533436/71‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:968724/71‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:3216173/71‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:3899779/71‑1 (MQ=255) aTCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAAcag > 1:3822874/1‑68 (MQ=255) aTCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa < 1:2550477/71‑1 (MQ=255) aGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:590360/1‑70 (MQ=255) aGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:3124829/68‑1 (MQ=255) aGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAAt < 1:310532/71‑1 (MQ=255) aGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAAt < 1:2070404/71‑1 (MQ=255) aGATCTTTGTAAACTGCGTAAGCCATGTAGAGTTCCATCATGGt > 1:2915336/1‑44 (MQ=255) gATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:2337203/67‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTg < 1:2020246/43‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:2238646/66‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:3634999/1‑71 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:870379/1‑71 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:1151704/1‑71 (MQ=255) aTCTTTGTAATATGCGTAAGCCATGTAGAGTTCCATAATGGTGAACTCTGGGTTATg < 1:1832478/57‑1 (MQ=255) cTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGAc > 1:617691/1‑57 (MQ=255) cTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:356757/1‑66 (MQ=255) gtggTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCtt > 1:371941/4‑71 (MQ=255) gtggTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCt > 1:485024/4‑70 (MQ=255) ttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATg > 1:3621195/1‑36 (MQ=255) ttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATg < 1:1406816/36‑1 (MQ=255) tggTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTc < 1:1457386/69‑1 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct < 1:2881580/44‑1 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc < 1:1380633/66‑1 (MQ=255) aaTCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTa > 1:1180402/1‑71 (MQ=255) aTCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTgg < 1:1988331/42‑1 (MQ=255) aTCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTa < 1:3319082/70‑1 (MQ=255) aTCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTa < 1:1777193/70‑1 (MQ=255) aTCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTAc > 1:3393670/1‑71 (MQ=255) tCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa < 1:213005/58‑1 (MQ=255) cTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACg > 1:1379981/1‑70 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:510700/1‑70 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:2380443/1‑70 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:2049827/1‑70 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:717129/1‑70 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:886015/1‑70 (MQ=255) cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACg > 1:363158/1‑67 (MQ=255) cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAg > 1:2787459/1‑71 (MQ=255) tAAGCCATGTTGAGTTCCATCATGGTGAACTCTTGGTTATGACGTACGGAAATACCTTCGt < 1:232386/61‑1 (MQ=255) tAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGt > 1:4001823/1‑70 (MQ=255) acgCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGATATACCTTCATTACGGAAGtt < 1:3033121/68‑1 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTa < 1:3801058/70‑1 (MQ=255) gCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACg < 1:1724533/71‑1 (MQ=255) ccATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg < 1:692887/40‑1 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:158487/71‑1 (MQ=255) tGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTg < 1:1984349/71‑1 (MQ=255) gTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATa < 1:895525/46‑1 (MQ=255) gTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATa < 1:3962637/46‑1 (MQ=255) gTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa > 1:2377412/1‑71 (MQ=255) tAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGAt > 1:1115048/1‑71 (MQ=255) tAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGAt > 1:3393682/1‑71 (MQ=255) tAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGAt > 1:3224739/1‑71 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:2329329/1‑59 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:2728770/1‑59 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACgg > 1:2990411/1‑66 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:1582271/70‑1 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt > 1:1419916/1‑71 (MQ=255) agagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:3603735/1‑45 (MQ=255) agagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACg > 1:1314361/1‑64 (MQ=255) agagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACg > 1:814838/1‑64 (MQ=255) agagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAtt > 1:2199845/1‑71 (MQ=255) gagTTCCATCATAGTGAACTCAGGATTATGGCGAACAGATATACCTTCATTACGGAAGtt < 1:3038220/60‑1 (MQ=255) agTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:3620293/1‑40 (MQ=255) agTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:142082/38‑1 (MQ=255) agTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTc < 1:1726181/71‑1 (MQ=255) gTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCg < 1:448984/71‑1 (MQ=255) gTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:1337063/71‑1 (MQ=255) ttCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCg > 1:575991/1‑70 (MQ=255) ttCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCg > 1:3700023/1‑70 (MQ=255) ttCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTAGGGAAGTTACGGTTGATTTCGa < 1:484488/71‑1 (MQ=255) ttCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACg < 1:2646181/51‑1 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:266554/1‑39 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:148517/1‑39 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTa > 1:1932021/1‑47 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGaa > 1:3558002/1‑70 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGaa > 1:3585177/1‑70 (MQ=255) catcatGGTGAACTCTGGGTTATGACGTAAGGAAATACCTTCGTTACg < 1:768854/48‑1 (MQ=255) atcatGGTGAACTCTGGGTTATGACGTAGGGAAATACCt < 1:3986201/39‑1 (MQ=255) tcatTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGa > 1:1826570/1‑60 (MQ=255) tcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGt < 1:787125/51‑1 (MQ=255) tcatGGTGAACTCTGGCTTATGACGTACGGAAATACCTTCGTTACg > 1:2526297/1‑46 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACg > 1:1956680/1‑44 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:3111264/1‑47 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:4009307/1‑47 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTa > 1:3432238/1‑51 (MQ=255) tGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:2631630/57‑1 (MQ=255) ggTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGc < 1:62105/70‑1 (MQ=255) | ACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGC > minE/1933546‑1933684 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |