| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,633 | A→G | 16.3% | R270R (CGT→CGC) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,633 | 0 | A | G | 16.3% | 354.7 / 58.7 | 147 | R270R (CGT→CGC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (68/55); new base G (16/8); total (84/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01 | |||||||||||
Only 100 of 148 total aligned reads displayed.
GCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCA > minE/1933566‑1933703 | gCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg < 1:3572921/70‑1 (MQ=255)gCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGt < 1:89657/71‑1 (MQ=255) gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGG‑TATGACGTACGGa < 1:1870443/71‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACTTACGGa < 1:533436/71‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:1979392/71‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:3216173/71‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:968724/71‑1 (MQ=255) aTCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa < 1:2550477/71‑1 (MQ=255) aGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAAt < 1:310532/71‑1 (MQ=255) aGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAAt < 1:2070404/71‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:870379/1‑71 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:3863377/1‑71 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:3634999/1‑71 (MQ=255) cTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGAc > 1:617691/1‑57 (MQ=255) gtggTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCtt > 1:371941/4‑71 (MQ=255) gtggTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCt > 1:485024/4‑70 (MQ=255) tggTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTc < 1:1457386/69‑1 (MQ=255) gTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGt > 1:2164326/1‑71 (MQ=255) tAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGt < 1:981975/70‑1 (MQ=255) aaTCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTa > 1:1180402/1‑71 (MQ=255) aTCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTa < 1:3319082/70‑1 (MQ=255) aTCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTa < 1:1777193/70‑1 (MQ=255) aTCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTAc > 1:3393670/1‑71 (MQ=255) aTCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTAc > 1:1745258/1‑71 (MQ=255) cTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACg > 1:1379981/1‑70 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:510700/1‑70 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:2049827/1‑70 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:2380443/1‑70 (MQ=255) tAAGCCATGTTGAGTTCCATCATGGTGAACTCTTGGTTATGACGTACGGAAATACCTTCGt < 1:232386/61‑1 (MQ=255) tAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGt > 1:4001823/1‑70 (MQ=255) acgCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGATATACCTTCATTACGGAAGtt < 1:3033121/68‑1 (MQ=255) gCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACg < 1:1724533/71‑1 (MQ=255) ccATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg < 1:692887/40‑1 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:3620610/71‑1 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:158487/71‑1 (MQ=255) gTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATa < 1:895525/46‑1 (MQ=255) gTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATa < 1:3962637/46‑1 (MQ=255) gTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa > 1:2377412/1‑71 (MQ=255) tAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGAt > 1:1115048/1‑71 (MQ=255) tAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGAt > 1:3224739/1‑71 (MQ=255) tAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGAt > 1:3393682/1‑71 (MQ=255) tAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGAt > 1:1436742/1‑71 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:3064225/41‑1 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:2329329/1‑59 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACgg > 1:1497251/1‑66 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:1582271/70‑1 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa > 1:1389863/1‑70 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt > 1:1419916/1‑71 (MQ=255) agagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAtt > 1:2199845/1‑71 (MQ=255) gagTTCCATCATAGTGAACTCAGGATTATGGCGAACAGATATACCTTCATTACGGAAGtt < 1:3038220/60‑1 (MQ=255) agTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTc > 1:1217848/1‑71 (MQ=255) agTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:3620293/1‑40 (MQ=255) gTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:1337063/71‑1 (MQ=255) ttCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCg > 1:3700023/1‑70 (MQ=255) ttCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCg > 1:575991/1‑70 (MQ=255) ttCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTAGGGAAGTTACGGTTGATTTCGa < 1:484488/71‑1 (MQ=255) ttCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACg < 1:2646181/51‑1 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:266554/1‑39 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTa > 1:1932021/1‑47 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGaa > 1:3558002/1‑70 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGaa > 1:3585177/1‑70 (MQ=255) catcatGGTGAACTCTGGGTTATGACGTAAGGAAATACCTTCGTTACg < 1:768854/48‑1 (MQ=255) atcatTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTc < 1:3645797/65‑1 (MQ=255) atcatGGTGAACTCTGGGTTATGACGTAGGGAAATACCt < 1:3986201/39‑1 (MQ=255) tcatTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGa > 1:1826570/1‑60 (MQ=255) tcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGt < 1:787125/51‑1 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:4009307/1‑47 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:3111264/1‑47 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTa > 1:3432238/1‑51 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAttt > 1:2737005/1‑61 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATacac < 1:3805667/70‑1 (MQ=255) tGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:2631630/57‑1 (MQ=255) tGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAtt > 1:2478740/1‑59 (MQ=255) tgTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGa < 1:2569018/61‑1 (MQ=255) ggTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGc < 1:62105/70‑1 (MQ=255) gTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACg > 1:1699411/1‑50 (MQ=255) gTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACg > 1:1042829/1‑50 (MQ=255) tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCTAATACACGCTc > 1:853191/1‑70 (MQ=255) tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTc > 1:3319114/1‑70 (MQ=255) tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTc > 1:4016534/1‑70 (MQ=255) tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg < 1:716624/71‑1 (MQ=255) gAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTc > 1:2771888/1‑57 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc > 1:1120992/1‑71 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc > 1:1974117/1‑71 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:3821955/71‑1 (MQ=255) ctctGGGATATGACGTACGGAAATACCTTCGTTACGGAAGTTACg < 1:2296696/45‑1 (MQ=255) tctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAtt < 1:233849/51‑1 (MQ=255) tctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGa > 1:3826753/1‑55 (MQ=255) ctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa > 1:1805626/1‑48 (MQ=255) ctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca > 1:406838/1‑71 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACgg > 1:2255360/1‑43 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAttt > 1:2623122/1‑50 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAttt > 1:1549634/1‑50 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGaa < 1:2343026/54‑1 (MQ=255) ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccacc < 1:2882579/70‑1 (MQ=255) gattATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCg < 1:2043347/48‑1 (MQ=255) tATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTc < 1:3911650/46‑1 (MQ=255) gACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAg > 1:523210/1‑59 (MQ=255) gACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAg > 1:2801985/1‑59 (MQ=255) aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAAccacca < 1:2439472/71‑1 (MQ=255) | GCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCA > minE/1933566‑1933703 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |