| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,639 | G→A | 15.2% | S268S (TCC→TCT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,639 | 0 | G | A | 15.2% | 395.5 / 52.3 | 151 | S268S (TCC→TCT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (69/58); new base A (15/8); total (84/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01 | |||||||||||
Only 100 of 154 total aligned reads displayed.
GATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGC > minE/1933570‑1933708 | gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGG‑TATGACGTACGGa < 1:1870443/71‑1 (MQ=255) aGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:590360/1‑70 (MQ=255) aGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:3124829/68‑1 (MQ=255) aGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAAt < 1:2070404/71‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:3863377/1‑71 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:3634999/1‑71 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:870379/1‑71 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc < 1:1380633/66‑1 (MQ=255) tAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGt < 1:981975/70‑1 (MQ=255) aaTCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTa > 1:1180402/1‑71 (MQ=255) aTCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTAc > 1:1745258/1‑71 (MQ=255) tCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa < 1:213005/58‑1 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:510700/1‑70 (MQ=255) cGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACg > 1:363158/1‑67 (MQ=255) tAAGCCATGTTGAGTTCCATCATGGTGAACTCTTGGTTATGACGTACGGAAATACCTTCGt < 1:232386/61‑1 (MQ=255) tAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGt > 1:4001823/1‑70 (MQ=255) acgCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGATATACCTTCATTACGGAAGtt < 1:3033121/68‑1 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:158487/71‑1 (MQ=255) tGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTg < 1:1984349/71‑1 (MQ=255) gTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATa < 1:3962637/46‑1 (MQ=255) gTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATa < 1:895525/46‑1 (MQ=255) gTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa > 1:2377412/1‑71 (MQ=255) tAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGAt > 1:3224739/1‑71 (MQ=255) tAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGAt > 1:3393682/1‑71 (MQ=255) tAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGAt > 1:1115048/1‑71 (MQ=255) tAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGAt > 1:1436742/1‑71 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:3064225/41‑1 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:2728770/1‑59 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:2329329/1‑59 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACgg > 1:1497251/1‑66 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa > 1:1389863/1‑70 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:1582271/70‑1 (MQ=255) agagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAtt > 1:2199845/1‑71 (MQ=255) agTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTc > 1:1217848/1‑71 (MQ=255) agTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:3620293/1‑40 (MQ=255) agTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:142082/38‑1 (MQ=255) agTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTc < 1:1726181/71‑1 (MQ=255) gTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTc > 1:671728/1‑70 (MQ=255) gTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCg < 1:448984/71‑1 (MQ=255) gTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:1337063/71‑1 (MQ=255) ttCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCg > 1:3700023/1‑70 (MQ=255) ttCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCg > 1:575991/1‑70 (MQ=255) ttCCATCATGGTGAACTCTGGGTTATGACGTACGGAAAt < 1:4010138/39‑1 (MQ=255) ttCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTAGGGAAGTTACGGTTGATTTCGa < 1:484488/71‑1 (MQ=255) ttCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:486289/70‑1 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATAc > 1:266554/1‑39 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTa > 1:2632872/1‑47 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTa > 1:1932021/1‑47 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGaa > 1:3585177/1‑70 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGaa > 1:3558002/1‑70 (MQ=255) catcatGGTGAACTCTGGGTTATGACGTAAGGAAATACCTTCGTTACg < 1:768854/48‑1 (MQ=255) atcatGGTGAACTCTGGGTTATGACGTAGGGAAATACCt < 1:3986201/39‑1 (MQ=255) tcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGt < 1:787125/51‑1 (MQ=255) tcatGGTGAACTCTGGCTTATGACGTACGGAAATACCTTCGTTACg > 1:2526297/1‑46 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:3111264/1‑47 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTa > 1:3432238/1‑51 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATacac < 1:3805667/70‑1 (MQ=255) tGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:2631630/57‑1 (MQ=255) tgTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGa < 1:2569018/61‑1 (MQ=255) gTGCACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGa > 1:3261736/1‑61 (MQ=255) gTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACg > 1:1042829/1‑50 (MQ=255) gTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACg > 1:1699411/1‑50 (MQ=255) gTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCgtt > 1:3387577/1‑68 (MQ=255) tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCTAATACACGCTc > 1:853191/1‑70 (MQ=255) tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTc > 1:3319114/1‑70 (MQ=255) tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTc > 1:4016534/1‑70 (MQ=255) tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg < 1:716624/71‑1 (MQ=255) gAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTc > 1:2771888/1‑57 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc > 1:1120992/1‑71 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc > 1:1974117/1‑71 (MQ=255) ctctGGGATATGACGTACGGAAATACCTTCGTTACGGAAGTTACg < 1:2296696/45‑1 (MQ=255) tctGGGTTATGACGTACGGAAATACCTTCTTTACGGAAGTTACGGtt < 1:1797267/47‑1 (MQ=255) tctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAtt < 1:233849/51‑1 (MQ=255) tctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGa > 1:3826753/1‑55 (MQ=255) ctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa > 1:1805626/1‑48 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACgg > 1:2255360/1‑43 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAttt > 1:1549634/1‑50 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGaa < 1:2343026/54‑1 (MQ=255) ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccacc < 1:2882579/70‑1 (MQ=255) gattATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCg < 1:2043347/48‑1 (MQ=255) gTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:3087285/49‑1 (MQ=255) ttATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAAccgccta > 1:261118/1‑68 (MQ=255) ttATGACGTACGGAAATACCTTCGTTTCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCaccaac < 1:530284/71‑1 (MQ=255) tATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTc < 1:3911650/46‑1 (MQ=255) tATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTc < 1:3686711/46‑1 (MQ=255) gACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg > 1:913760/1‑56 (MQ=255) gACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAg > 1:242739/1‑59 (MQ=255) gACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAg > 1:2801985/1‑59 (MQ=255) aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAAccacca < 1:2439472/71‑1 (MQ=255) tACTGAAATACCTTCGTTACGGAAGTTACGGTTGAt < 1:2546693/36‑1 (MQ=255) tACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:3031249/40‑1 (MQ=255) tACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:133992/40‑1 (MQ=255) tACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:3582918/40‑1 (MQ=255) tACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:1709171/40‑1 (MQ=255) tACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac < 1:217576/66‑1 (MQ=255) aCGGAAATACCTTCGTTACGGAAGTTAGGGTTGATTTCg < 1:374537/39‑1 (MQ=255) cGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGc > 1:1033318/1‑71 (MQ=255) ggAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:1473871/37‑1 (MQ=255) ggAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:3605211/37‑1 (MQ=255) ggAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGc > 1:3762003/1‑70 (MQ=255) | GATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGC > minE/1933570‑1933708 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |