| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,687 | G→A | 17.6% | F252F (TTC→TTT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,687 | 0 | G | A | 17.6% | 178.9 / 28.9 | 74 | F252F (TTC→TTT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (34/27); new base A (11/2); total (45/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.54e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAG > minE/1933617‑1933755 | tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg < 1:716624/71‑1 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc > 1:1974117/1‑71 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc > 1:1120992/1‑71 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:3821955/71‑1 (MQ=255) ctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca > 1:406838/1‑71 (MQ=255) ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccacc < 1:2882579/70‑1 (MQ=255) ttATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAAccgccta > 1:261118/1‑68 (MQ=255) ttATGACGTACGGAAATACCTTCGTTTCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCaccaac < 1:530284/71‑1 (MQ=255) aTGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCaccaacca > 1:989482/1‑71 (MQ=255) gACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg > 1:913760/1‑56 (MQ=255) gACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAg > 1:523210/1‑59 (MQ=255) gACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAg > 1:2801985/1‑59 (MQ=255) gACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAg > 1:242739/1‑59 (MQ=255) aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAAccacca < 1:2439472/71‑1 (MQ=255) cGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac > 1:3973233/1‑68 (MQ=255) tACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac < 1:217576/66‑1 (MQ=255) aCGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACg > 1:108448/1‑71 (MQ=255) cGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGc > 1:1033318/1‑71 (MQ=255) ggAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGc > 1:3762003/1‑70 (MQ=255) gAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCaccaa > 1:4026208/1‑58 (MQ=255) aaaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTg < 1:3909333/71‑1 (MQ=255) aaaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTg < 1:2878222/71‑1 (MQ=255) aaaTACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt > 1:3601423/1‑70 (MQ=255) aaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGa < 1:10027/71‑1 (MQ=255) aaTACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt > 1:3770173/1‑69 (MQ=255) aaTACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt > 1:3167801/1‑69 (MQ=255) tACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt > 1:56374/1‑67 (MQ=255) aCCTTCGTAACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCaccaac > 1:3004721/1‑54 (MQ=255) ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:1915596/42‑1 (MQ=255) ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:341326/42‑1 (MQ=255) ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:2806749/42‑1 (MQ=255) ttCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:1493998/42‑1 (MQ=255) tCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca > 1:1316429/1‑45 (MQ=255) tCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGcca > 1:1037450/1‑45 (MQ=255) tCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAg > 1:1538647/1‑71 (MQ=255) gTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGtt > 1:1418443/1‑71 (MQ=255) ttACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAg < 1:4071597/53‑1 (MQ=255) ttACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTc < 1:312358/71‑1 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAga > 1:2041964/1‑60 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCg > 1:53451/1‑71 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCg > 1:255040/1‑71 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCg > 1:3490400/1‑71 (MQ=255) cGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCgg > 1:203821/1‑71 (MQ=255) cGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCg < 1:2881085/70‑1 (MQ=255) aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc > 1:2449018/1‑71 (MQ=255) aGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc > 1:1448985/1‑70 (MQ=255) aGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc > 1:2900841/1‑70 (MQ=255) gTTACGGTTGATTTCGAATACACGCTCGAAGCCaccaac < 1:2432471/39‑1 (MQ=255) gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGa < 1:3098223/71‑1 (MQ=255) aCGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATAc > 1:3611396/1‑71 (MQ=255) cGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGa < 1:451250/67‑1 (MQ=255) cGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACg < 1:803030/71‑1 (MQ=255) cGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACg < 1:807925/71‑1 (MQ=255) ggTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCAGGCGCGATACg < 1:4092885/70‑1 (MQ=255) ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc < 1:193866/71‑1 (MQ=255) ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc < 1:3872398/71‑1 (MQ=255) ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc < 1:3029277/71‑1 (MQ=255) ttGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACa > 1:1208894/1‑52 (MQ=255) tGATTTCGAATACACGCTCGAAGCCACCAAccacca > 1:741788/1‑36 (MQ=255) aTTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa > 1:3631984/1‑71 (MQ=255) tttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGa > 1:3823431/1‑60 (MQ=255) ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTc > 1:776922/1‑51 (MQ=255) ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa > 1:3673052/1‑71 (MQ=255) tCGAATACACGCTCGACGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACAt > 1:1742955/1‑71 (MQ=255) cGAATACACGCTCGAAGCCACCAACCAACAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACAt < 1:2267886/70‑1 (MQ=255) gAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACAtat > 1:4106411/1‑69 (MQ=255) gAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACAta > 1:3495070/1‑69 (MQ=255) gAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACAta > 1:3100084/1‑69 (MQ=255) tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc < 1:1808327/40‑1 (MQ=255) tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc < 1:1927913/40‑1 (MQ=255) tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga < 1:3602721/71‑1 (MQ=255) cacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa > 1:2419638/1‑63 (MQ=255) cacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc > 1:744424/1‑67 (MQ=255) cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGa < 1:3037342/49‑1 (MQ=255) cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc > 1:1476339/1‑71 (MQ=255) tCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAg > 1:1432559/1‑71 (MQ=255) gAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc < 1:3342719/60‑1 (MQ=255) | TGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAG > minE/1933617‑1933755 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |