| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 121,875 | C→T | 12.1% | A18V (GCT→GTT) | cueO → | multicopper oxidase (laccase) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 121,875 | 0 | C | T | 12.1% | 210.5 / 16.1 | 74 | A18V (GCT→GTT) | cueO | multicopper oxidase (laccase) |
| Reads supporting (aligned to +/- strand): ref base C (23/42); new base T (5/4); total (28/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGAATAAGGAAATAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTACCGATCCCTGATTTGCTCAC > minE/121805‑121944 | aaGAATAAGGAAATAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGc > 1:590621/1‑71 (MQ=255) aGAATAAGGAAATAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGc < 1:1614044/70‑1 (MQ=255) aGAATAAGGAAATAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCt < 1:1863462/71‑1 (MQ=255) gAATAAGGAAATAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCtt > 1:1489593/1‑71 (MQ=255) aaTAAGGAAATAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCttt < 1:84301/71‑1 (MQ=255) tAAGGAAATAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGc > 1:3417050/1‑71 (MQ=255) tAAGGAAATAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCCTCGGCTTTGc < 1:3414074/71‑1 (MQ=255) aGGAAATAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGcc < 1:1747676/70‑1 (MQ=255) gAAATAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGTTTTGCCGCt < 1:1994481/71‑1 (MQ=255) gAAATAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCt < 1:1491594/71‑1 (MQ=255) aaTAACTATGCAACGTCGTGATTTCTTAAAATATTGCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCtgt < 1:2424829/71‑1 (MQ=255) aaTAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCtgt > 1:1727743/1‑71 (MQ=255) aaTAACTACGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCtgt > 1:148638/1‑71 (MQ=255) aTAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCtgtg > 1:156789/1‑71 (MQ=255) aTAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCtgtg < 1:1982421/71‑1 (MQ=255) aTAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCtgtg < 1:3906097/71‑1 (MQ=255) tAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCtgtg < 1:1645349/70‑1 (MQ=255) aTGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCtgtg < 1:2183505/65‑1 (MQ=255) tGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGcc < 1:3843463/69‑1 (MQ=255) aCGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCttt < 1:184344/51‑1 (MQ=255) aCGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCtgt < 1:1404052/59‑1 (MQ=255) aCGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCtgt < 1:1805622/59‑1 (MQ=255) aCGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCtgt < 1:2114768/59‑1 (MQ=255) cgtcgtGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGt < 1:3824551/71‑1 (MQ=255) cgtcgtGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGt < 1:3555955/71‑1 (MQ=255) cgtcgtGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGt < 1:1889771/71‑1 (MQ=255) gtcgtGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTa > 1:33125/1‑71 (MQ=255) gtGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTAtt < 1:3565871/70‑1 (MQ=255) gATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCg < 1:3482592/48‑1 (MQ=255) gATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGc < 1:2129737/49‑1 (MQ=255) gATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGc > 1:1617110/1‑71 (MQ=255) gATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGc > 1:463178/1‑71 (MQ=255) aTTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGTTTTGCCGCTGTGGAGCCGCGCAGTATTTGCg > 1:2802018/1‑71 (MQ=255) ttCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCtgtg > 1:224514/1‑50 (MQ=255) ttCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCtgtg > 1:2303756/1‑50 (MQ=255) cTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCtgtg > 1:1870221/1‑48 (MQ=255) tAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGaa > 1:3174185/1‑71 (MQ=255) aaaaTATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAg < 1:2075761/68‑1 (MQ=255) aaaaTATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGaa < 1:3211729/70‑1 (MQ=255) aaaTATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAAc < 1:3428258/70‑1 (MQ=255) aaaTATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAAc < 1:3686485/70‑1 (MQ=255) aaTATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGc < 1:952732/71‑1 (MQ=255) aaTATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGc < 1:965033/71‑1 (MQ=255) tatTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTgg < 1:1038330/42‑1 (MQ=255) tatTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCa > 1:1412000/1‑63 (MQ=255) tatTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGccc < 1:3144369/71‑1 (MQ=255) atTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAg < 1:1051607/51‑1 (MQ=255) ttCCGTCGCGCTGGGTGTGGCTTCGGTTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAg < 1:1932405/62‑1 (MQ=255) ttCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCa < 1:1365535/70‑1 (MQ=255) ttCCGTCACGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCaa > 1:4064649/1‑71 (MQ=255) cGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGt < 1:113711/71‑1 (MQ=255) tCGCGCTGGGTGTGGCTTCGGTTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTa < 1:3709621/71‑1 (MQ=255) tCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTa < 1:3420762/47‑1 (MQ=255) gcgcTGGGTGTGGCTTCGGTTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTAcc > 1:1007277/1‑71 (MQ=255) gcgcTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTAcc < 1:3165303/71‑1 (MQ=255) cTGGGTGTGGCTTCGGTTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGaa > 1:441068/1‑54 (MQ=255) cTGGGTGTGGCTTCGGTTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGaa > 1:1314121/1‑54 (MQ=255) cTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCAGCAGAACGCCCAACGTTACCGAt < 1:1265310/71‑1 (MQ=255) tGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTa < 1:126781/41‑1 (MQ=255) ggTGTGGCTTCGGTTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGt > 1:2534492/1‑61 (MQ=255) gtgtGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGaa < 1:1994296/50‑1 (MQ=255) gtgtGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTACCGATCCCt > 1:3639307/1‑71 (MQ=255) tgGCTTCGGTTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTACCGATCCCTGa < 1:917214/70‑1 (MQ=255) tgGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTACCGATCCCTGAt > 1:2927940/1‑71 (MQ=255) gCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGCACg > 1:142938/1‑47 (MQ=255) gCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTACCGATCCCTGAttt > 1:290740/1‑71 (MQ=255) gCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTACCGATCCCTGAttt > 1:2824085/1‑71 (MQ=255) gCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTACCGATCCCTGAtt < 1:3445035/70‑1 (MQ=255) gCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTACCGATCCCTGAtt < 1:178068/70‑1 (MQ=255) cTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTACCGATCCCTGATTTg < 1:3286603/71‑1 (MQ=255) ttCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTACCGATCCCTGATTTGc > 1:3517488/1‑71 (MQ=255) cGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTACCGATCCCTGATTTGCt < 1:262364/70‑1 (MQ=255) ggCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTACCGATCCCTGATTTGCTc < 1:3793248/70‑1 (MQ=255) ggCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTACCGATCCCTGATTTGCTCa > 1:3352028/1‑71 (MQ=255) ggCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTACCGATCCCTGATTTGCTCa > 1:3469811/1‑71 (MQ=255) gCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTACCGATCCCTGATTTGCTCAc > 1:1697881/1‑71 (MQ=255) | AAGAATAAGGAAATAACTATGCAACGTCGTGATTTCTTAAAATATTCCGTCGCGCTGGGTGTGGCTTCGGCTTTGCCGCTGTGGAGCCGCGCAGTATTTGCGGCAGAACGCCCAACGTTACCGATCCCTGATTTGCTCAC > minE/121805‑121944 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |