| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,699 | C→A | 19.9% | V248V (GTG→GTT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,699 | 0 | C | A | 19.9% | 171.9 / 19.4 | 60 | V248V (GTG→GTT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (23/25); new base A (10/2); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
ATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGG > minE/1933630‑1933769 | aTGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCaccaacca > 1:989482/1‑71 (MQ=255) aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAAccacca < 1:2439472/71‑1 (MQ=255) cGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac > 1:3973233/1‑68 (MQ=255) tACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac < 1:217576/66‑1 (MQ=255) aCGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACg > 1:108448/1‑71 (MQ=255) cGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGc > 1:1033318/1‑71 (MQ=255) ggAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGc > 1:3762003/1‑70 (MQ=255) aaaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTg < 1:3909333/71‑1 (MQ=255) aaaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTg < 1:2878222/71‑1 (MQ=255) aaaTACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt > 1:3601423/1‑70 (MQ=255) aaTACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGa < 1:10027/71‑1 (MQ=255) aaTACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt > 1:3770173/1‑69 (MQ=255) aaTACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt > 1:3167801/1‑69 (MQ=255) tACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGttt > 1:56374/1‑67 (MQ=255) tCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAg > 1:1538647/1‑71 (MQ=255) gTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGtt > 1:1418443/1‑71 (MQ=255) ttACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAg < 1:4071597/53‑1 (MQ=255) ttACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTc < 1:312358/71‑1 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAga > 1:2041964/1‑60 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCg > 1:53451/1‑71 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCg > 1:3490400/1‑71 (MQ=255) aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCg > 1:255040/1‑71 (MQ=255) cGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCgg > 1:203821/1‑71 (MQ=255) cGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCg < 1:2881085/70‑1 (MQ=255) aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc > 1:2449018/1‑71 (MQ=255) aGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc > 1:2900841/1‑70 (MQ=255) aGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc > 1:1448985/1‑70 (MQ=255) gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGa < 1:3098223/71‑1 (MQ=255) aCGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATAc > 1:3611396/1‑71 (MQ=255) cGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGa < 1:451250/67‑1 (MQ=255) cGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACg < 1:807925/71‑1 (MQ=255) cGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACg < 1:803030/71‑1 (MQ=255) ggTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCAGGCGCGATACg < 1:4092885/70‑1 (MQ=255) ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc < 1:193866/71‑1 (MQ=255) ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc < 1:3029277/71‑1 (MQ=255) ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc < 1:3872398/71‑1 (MQ=255) ttGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACa > 1:1208894/1‑52 (MQ=255) tGATTTCGAATACACGCTCGAAGCCACCAAccacca > 1:741788/1‑36 (MQ=255) aTTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa > 1:3631984/1‑71 (MQ=255) tttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGa > 1:3823431/1‑60 (MQ=255) ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTc > 1:776922/1‑51 (MQ=255) ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa > 1:3673052/1‑71 (MQ=255) tCGAATACACGCTCGACGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACAt > 1:1742955/1‑71 (MQ=255) cGAATACACGCTCGAAGCCACCAACCAACAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACAt < 1:2267886/70‑1 (MQ=255) gAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACAtat > 1:4106411/1‑69 (MQ=255) gAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACAta > 1:3100084/1‑69 (MQ=255) gAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACAta > 1:3495070/1‑69 (MQ=255) tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc < 1:1808327/40‑1 (MQ=255) tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc < 1:1927913/40‑1 (MQ=255) tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga < 1:3602721/71‑1 (MQ=255) cacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa > 1:2419638/1‑63 (MQ=255) cacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc > 1:744424/1‑67 (MQ=255) cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGa < 1:3037342/49‑1 (MQ=255) cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc > 1:1476339/1‑71 (MQ=255) tCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAg > 1:1432559/1‑71 (MQ=255) gAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc < 1:3342719/60‑1 (MQ=255) aGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg > 1:4067940/1‑71 (MQ=255) gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCTCGATACGCAGGTACATGTCGAGATCCAGCGCGt < 1:47521/71‑1 (MQ=255) gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt < 1:3471807/71‑1 (MQ=255) gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt > 1:2937929/1‑71 (MQ=255) ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa < 1:3563919/50‑1 (MQ=255) ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa < 1:1699890/50‑1 (MQ=255) ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGtt < 1:2867274/71‑1 (MQ=255) caccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa < 1:2427662/49‑1 (MQ=255) caccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa < 1:2265559/49‑1 (MQ=255) accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt < 1:3777217/71‑1 (MQ=255) accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt < 1:940860/71‑1 (MQ=255) ccaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAgg < 1:2162682/45‑1 (MQ=255) ccaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAgcgc < 1:1302375/65‑1 (MQ=255) ccaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtg < 1:1778782/71‑1 (MQ=255) aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa > 1:3974055/1‑45 (MQ=255) aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt < 1:4006010/71‑1 (MQ=255) aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt > 1:2263193/1‑71 (MQ=255) accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtg > 1:860261/1‑71 (MQ=255) accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt > 1:4068966/1‑70 (MQ=255) caccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtggg > 1:1790564/1‑71 (MQ=255) | ATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGG > minE/1933630‑1933769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |