Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,714 G→A 11.8% Y243Y (TAC→TAT lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,7140GA11.8% 218.2 / 15.5 76Y243Y (TAC→TATlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (34/33);  new base A (7/2);  total (41/35)
Fisher's exact test for biased strand distribution p-value = 1.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAA  >  minE/1933649‑1933775
                                                                 |                                                             
tCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAg                                                          >  1:1538647/1‑71 (MQ=255)
  gTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGtt                                                        >  1:1418443/1‑71 (MQ=255)
   ttACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTc                                                       <  1:312358/71‑1 (MQ=255)
     aCGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCg                                                     >  1:53451/1‑71 (MQ=255)
     aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCg                                                     >  1:255040/1‑71 (MQ=255)
     aCGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCg                                                     >  1:3490400/1‑71 (MQ=255)
      cGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCgg                                                    >  1:203821/1‑71 (MQ=255)
      cGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCg                                                     <  1:2881085/70‑1 (MQ=255)
         aaGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc                                                 >  1:2449018/1‑71 (MQ=255)
          aGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc                                                 >  1:1448985/1‑70 (MQ=255)
          aGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGgcgc                                                 >  1:2900841/1‑70 (MQ=255)
           gTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGa                                               <  1:3098223/71‑1 (MQ=255)
              aCGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATAc                                            >  1:3611396/1‑71 (MQ=255)
               cGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGa                                               <  1:451250/67‑1 (MQ=255)
               cGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACg                                           <  1:803030/71‑1 (MQ=255)
               cGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACg                                           <  1:807925/71‑1 (MQ=255)
                ggTTGATTTCGAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCAGGCGCGATACg                                           <  1:4092885/70‑1 (MQ=255)
                ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc                                          <  1:3872398/71‑1 (MQ=255)
                ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc                                          <  1:3029277/71‑1 (MQ=255)
                ggTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGc                                          <  1:193866/71‑1 (MQ=255)
                  ttGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACa                                                           >  1:1208894/1‑52 (MQ=255)
                     aTTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa                                     >  1:3631984/1‑71 (MQ=255)
                      tttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGa                                               >  1:3823431/1‑60 (MQ=255)
                       ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTc                                                       >  1:776922/1‑51 (MQ=255)
                       ttCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa                                   >  1:3673052/1‑71 (MQ=255)
                        tCGAATACACGCTCGACGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACAt                                  >  1:1742955/1‑71 (MQ=255)
                         cGAATACACGCTCGAAGCCACCAACCAACAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACAt                                  <  1:2267886/70‑1 (MQ=255)
                          gAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACAtat                                >  1:4106411/1‑69 (MQ=255)
                          gAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACAta                                 >  1:3495070/1‑69 (MQ=255)
                          gAATACCCGTTCAAAACCGCCTACAACCAGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACAta                                 >  1:3100084/1‑69 (MQ=255)
                             tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc                                                            <  1:1808327/40‑1 (MQ=255)
                             tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTAc                                                            <  1:1927913/40‑1 (MQ=255)
                             tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga                             <  1:3602721/71‑1 (MQ=255)
                               cacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACa                                   >  1:2419638/1‑63 (MQ=255)
                               cacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc                               >  1:744424/1‑67 (MQ=255)
                                 cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGa                                               <  1:3037342/49‑1 (MQ=255)
                                 cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc                         >  1:1476339/1‑71 (MQ=255)
                                    tCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAg                      >  1:1432559/1‑71 (MQ=255)
                                      gAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc                               <  1:3342719/60‑1 (MQ=255)
                                        aGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg                  >  1:4067940/1‑71 (MQ=255)
                                         gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCTCGATACGCAGGTACATGTCGAGATCCAGCGCGt                 <  1:47521/71‑1 (MQ=255)
                                         gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                 >  1:2937929/1‑71 (MQ=255)
                                         gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                 <  1:3471807/71‑1 (MQ=255)
                                          ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa                                     <  1:3563919/50‑1 (MQ=255)
                                          ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa                                     <  1:1699890/50‑1 (MQ=255)
                                          ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGtt                <  1:2867274/71‑1 (MQ=255)
                                           caccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa                                     <  1:2265559/49‑1 (MQ=255)
                                           caccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa                                     <  1:2427662/49‑1 (MQ=255)
                                            accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt              <  1:940860/71‑1 (MQ=255)
                                            accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt              <  1:3777217/71‑1 (MQ=255)
                                             ccaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAgg                                       <  1:2162682/45‑1 (MQ=255)
                                             ccaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAgcgc                   <  1:1302375/65‑1 (MQ=255)
                                             ccaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtg             <  1:1778782/71‑1 (MQ=255)
                                               aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa                                     >  1:3974055/1‑45 (MQ=255)
                                               aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt           >  1:2263193/1‑71 (MQ=255)
                                               aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt           <  1:4006010/71‑1 (MQ=255)
                                                accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtg          >  1:860261/1‑71 (MQ=255)
                                                accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt           >  1:4068966/1‑70 (MQ=255)
                                                 caaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtgatgg         >  1:3355044/3‑71 (MQ=255)
                                                 caaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtgatgg         >  1:2820590/3‑71 (MQ=255)
                                                  caccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtggg        >  1:1790564/1‑71 (MQ=255)
                                                   accaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTa                                     <  1:188923/41‑1 (MQ=255)
                                                   accaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa                       >  1:723509/1‑55 (MQ=255)
                                                    ccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtg          <  1:3342585/67‑1 (MQ=255)
                                                    ccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggtg      <  1:3637099/71‑1 (MQ=255)
                                                    ccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggt       >  1:2704845/1‑70 (MQ=255)
                                                      aGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggtg      >  1:1325526/1‑69 (MQ=255)
                                                       gACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa                       >  1:118621/1‑51 (MQ=255)
                                                       gACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa   <  1:146971/71‑1 (MQ=255)
                                                        aCGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGtaataa  <  1:708954/71‑1 (MQ=255)
                                                        aCGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATaa  <  1:1254616/71‑1 (MQ=255)
                                                         cGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa                       >  1:727859/1‑49 (MQ=255)
                                                          gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt           >  1:3728706/1‑60 (MQ=255)
                                                            ttGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtg          >  1:2975726/1‑59 (MQ=255)
                                                              gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTa               >  1:2845528/1‑52 (MQ=255)
                                                              gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtg             >  1:4072739/1‑54 (MQ=255)
                                                                 |                                                             
TCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAA  >  minE/1933649‑1933775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: