Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,744 G→A 7.9% D233D (GAC→GAT lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,7440GA 7.9% 245.0 / 8.3 76D233D (GAC→GATlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (33/37);  new base A (3/3);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCAC  >  minE/1933678‑1933806
                                                                  |                                                              
tACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCga                                                            <  1:3602721/71‑1 (MQ=255)
  cacGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc                                                              >  1:744424/1‑67 (MQ=255)
    cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc                                                        >  1:1476339/1‑71 (MQ=255)
       tCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAg                                                     >  1:1432559/1‑71 (MQ=255)
         gAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTc                                                              <  1:3342719/60‑1 (MQ=255)
           aGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg                                                 >  1:4067940/1‑71 (MQ=255)
            gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCTCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                <  1:47521/71‑1 (MQ=255)
            gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                >  1:2937929/1‑71 (MQ=255)
            gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                <  1:3471807/71‑1 (MQ=255)
             ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGtt                                               <  1:2867274/71‑1 (MQ=255)
               accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                                             <  1:3777217/71‑1 (MQ=255)
               accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                                             <  1:940860/71‑1 (MQ=255)
                ccaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAgcgc                                                  <  1:1302375/65‑1 (MQ=255)
                ccaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtg                                            <  1:1778782/71‑1 (MQ=255)
                  aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt                                          <  1:4006010/71‑1 (MQ=255)
                  aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt                                          >  1:2263193/1‑71 (MQ=255)
                   accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtg                                         >  1:860261/1‑71 (MQ=255)
                   accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt                                          >  1:4068966/1‑70 (MQ=255)
                    caaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtgatgg                                        >  1:3355044/3‑71 (MQ=255)
                    caaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtgatgg                                        >  1:2820590/3‑71 (MQ=255)
                     caccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtggg                                       >  1:1790564/1‑71 (MQ=255)
                      accaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa                                                      >  1:723509/1‑55 (MQ=255)
                       ccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtg                                         <  1:3342585/67‑1 (MQ=255)
                       ccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggtg                                     <  1:3637099/71‑1 (MQ=255)
                       ccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggt                                      >  1:2704845/1‑70 (MQ=255)
                         aGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggtg                                     >  1:1325526/1‑69 (MQ=255)
                          gACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa                                                      >  1:118621/1‑51 (MQ=255)
                          gACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                  <  1:146971/71‑1 (MQ=255)
                           aCGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGtaataa                                 <  1:708954/71‑1 (MQ=255)
                           aCGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATaa                                 <  1:1254616/71‑1 (MQ=255)
                            cGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa                                                      >  1:727859/1‑49 (MQ=255)
                             gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt                                          >  1:3728706/1‑60 (MQ=255)
                               ttGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtg                                         >  1:2975726/1‑59 (MQ=255)
                                 gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTa                                              >  1:2845528/1‑52 (MQ=255)
                                 gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtg                                            >  1:4072739/1‑54 (MQ=255)
                                       cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                >  1:2624653/1‑44 (MQ=255)
                                       cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCa                      <  1:1590013/70‑1 (MQ=255)
                                       cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                     >  1:2046856/1‑71 (MQ=255)
                                        aGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATaaa                                <  1:3268151/59‑1 (MQ=255)
                                        aGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGc                    <  1:2405991/71‑1 (MQ=255)
                                         gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATaaa                                <  1:3847449/58‑1 (MQ=255)
                                          ttCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCgg                  >  1:662680/1‑71 (MQ=255)
                                           tCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtg                                            >  1:4006402/1‑44 (MQ=16)
                                            ccGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                                             >  1:1645162/1‑42 (MQ=255)
                                            ccGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                                             >  1:799523/1‑42 (MQ=255)
                                            ccGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAAc                               >  1:3298229/1‑56 (MQ=255)
                                            ccGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCa                >  1:237568/1‑71 (MQ=255)
                                             cGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCAc               <  1:3474181/71‑1 (MQ=255)
                                             cGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCAc               <  1:870545/71‑1 (MQ=255)
                                             cGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAt                                             <  1:3633974/41‑1 (MQ=16)
                                              ggCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCgg                  <  1:3369360/67‑1 (MQ=255)
                                              ggCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCAcc              <  1:3105635/71‑1 (MQ=255)
                                              ggCGCGATACGCAGATACATATCTAAATCCAgagca                                                 <  1:98655/36‑2 (MQ=12)
                                               gcgcGATACGCAGGTACATGTCGAGATCCAGCGCGTTa                                              <  1:2051441/38‑1 (MQ=255)
                                                cgcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGc            <  1:2667051/71‑1 (MQ=255)
                                                 gcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc           <  1:3123512/71‑1 (MQ=255)
                                                   gATACTCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                              <  1:131490/50‑1 (MQ=255)
                                                   gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                              <  1:3825922/50‑1 (MQ=255)
                                                     tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGa                            >  1:2506259/1‑50 (MQ=255)
                                                     tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGc            <  1:2999373/66‑1 (MQ=255)
                                                     tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg         <  1:838136/69‑1 (MQ=255)
                                                      aCGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                     >  1:1155076/1‑56 (MQ=255)
                                                      aCGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCa          >  1:142193/1‑67 (MQ=255)
                                                       cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                     >  1:3541252/1‑55 (MQ=255)
                                                       cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa      <  1:1136795/70‑1 (MQ=255)
                                                        gCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATc    <  1:1747792/71‑1 (MQ=255)
                                                        gCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATc    <  1:884411/71‑1 (MQ=255)
                                                          aGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCAc  <  1:2002269/71‑1 (MQ=255)
                                                            gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                  <  1:3805697/37‑1 (MQ=255)
                                                            gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAggg       <  1:1973442/64‑1 (MQ=255)
                                                             tACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGc            <  1:93284/58‑1 (MQ=255)
                                                              aCATGTCGAGATCCAGCGCGTTATGGTGGGTGATaaa                                <  1:1291034/37‑1 (MQ=255)
                                                              aCATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGa                            >  1:1211037/1‑41 (MQ=255)
                                                              aCATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCa                      >  1:182780/1‑47 (MQ=255)
                                                              aCATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCa          >  1:1299086/1‑59 (MQ=255)
                                                                aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                              >  1:1346350/1‑37 (MQ=255)
                                                                  gTCGAGATACAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATc    >  1:2050277/1‑61 (MQ=255)
                                                                  |                                                              
TACACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCAC  >  minE/1933678‑1933806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: