Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,749 G→A 8.1% L232F (CTC→TTC) ‡ lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,7490GA 8.1% 238.1 / 7.5 74L232F (CTC→TTC) ‡lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (33/35);  new base A (3/3);  total (36/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGC  >  minE/1933682‑1933819
                                                                   |                                                                      
cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc                                                                     >  1:1476339/1‑71 (MQ=255)
   tCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAg                                                                  >  1:1432559/1‑71 (MQ=255)
       aGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg                                                              >  1:4067940/1‑71 (MQ=255)
        gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCTCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                             <  1:47521/71‑1 (MQ=255)
        gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                             <  1:3471807/71‑1 (MQ=255)
        gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                             >  1:2937929/1‑71 (MQ=255)
         ccaccaACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGtt                                                            <  1:2867274/71‑1 (MQ=255)
           accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                                                          <  1:940860/71‑1 (MQ=255)
           accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                                                          <  1:3777217/71‑1 (MQ=255)
            ccaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAgcgc                                                               <  1:1302375/65‑1 (MQ=255)
            ccaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtg                                                         <  1:1778782/71‑1 (MQ=255)
              aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt                                                       <  1:4006010/71‑1 (MQ=255)
              aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt                                                       >  1:2263193/1‑71 (MQ=255)
               accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtg                                                      >  1:860261/1‑71 (MQ=255)
               accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt                                                       >  1:4068966/1‑70 (MQ=255)
                caaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtgatgg                                                     >  1:3355044/3‑71 (MQ=255)
                caaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtgatgg                                                     >  1:2820590/3‑71 (MQ=255)
                 caccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtggg                                                    >  1:1790564/1‑71 (MQ=255)
                  accaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa                                                                   >  1:723509/1‑55 (MQ=255)
                   ccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtg                                                      <  1:3342585/67‑1 (MQ=255)
                   ccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggtg                                                  <  1:3637099/71‑1 (MQ=255)
                   ccaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggt                                                   >  1:2704845/1‑70 (MQ=255)
                     aGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggtg                                                  >  1:1325526/1‑69 (MQ=255)
                      gACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa                                                                   >  1:118621/1‑51 (MQ=255)
                      gACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                               <  1:146971/71‑1 (MQ=255)
                       aCGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGtaataa                                              <  1:708954/71‑1 (MQ=255)
                       aCGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATaa                                              <  1:1254616/71‑1 (MQ=255)
                        cGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa                                                                   >  1:727859/1‑49 (MQ=255)
                         gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt                                                       >  1:3728706/1‑60 (MQ=255)
                           ttGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggtg                                                      >  1:2975726/1‑59 (MQ=255)
                             gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTa                                                           >  1:2845528/1‑52 (MQ=255)
                             gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtg                                                         >  1:4072739/1‑54 (MQ=255)
                                   cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                             >  1:2624653/1‑44 (MQ=255)
                                   cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCa                                   <  1:1590013/70‑1 (MQ=255)
                                   cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                  >  1:2046856/1‑71 (MQ=255)
                                    aGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATaaa                                             <  1:3268151/59‑1 (MQ=255)
                                    aGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGc                                 <  1:2405991/71‑1 (MQ=255)
                                     gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATaaa                                             <  1:3847449/58‑1 (MQ=255)
                                      ttCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCgg                               >  1:662680/1‑71 (MQ=255)
                                       tCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtg                                                         >  1:4006402/1‑44 (MQ=16)
                                        ccGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                                                          >  1:1645162/1‑42 (MQ=255)
                                        ccGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                                                          >  1:799523/1‑42 (MQ=255)
                                        ccGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAAc                                            >  1:3298229/1‑56 (MQ=255)
                                        ccGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCa                             >  1:237568/1‑71 (MQ=255)
                                         cGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCAc                            <  1:870545/71‑1 (MQ=255)
                                         cGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCAc                            <  1:3474181/71‑1 (MQ=255)
                                         cGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAt                                                          <  1:3633974/41‑1 (MQ=16)
                                          ggCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCgg                               <  1:3369360/67‑1 (MQ=255)
                                          ggCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCAcc                           <  1:3105635/71‑1 (MQ=255)
                                          ggCGCGATACGCAGATACATATCTAAATCCAgagca                                                              <  1:98655/36‑2 (MQ=12)
                                           gcgcGATACGCAGGTACATGTCGAGATCCAGCGCGTTa                                                           <  1:2051441/38‑1 (MQ=255)
                                            cgcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGc                         <  1:2667051/71‑1 (MQ=255)
                                             gcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc                        <  1:3123512/71‑1 (MQ=255)
                                               gATACTCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                                           <  1:131490/50‑1 (MQ=255)
                                               gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                                           <  1:3825922/50‑1 (MQ=255)
                                                 tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGa                                         >  1:2506259/1‑50 (MQ=255)
                                                 tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGc                         <  1:2999373/66‑1 (MQ=255)
                                                 tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg                      <  1:838136/69‑1 (MQ=255)
                                                  aCGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                  >  1:1155076/1‑56 (MQ=255)
                                                  aCGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCa                       >  1:142193/1‑67 (MQ=255)
                                                   cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                  >  1:3541252/1‑55 (MQ=255)
                                                   cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa                   <  1:1136795/70‑1 (MQ=255)
                                                    gCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATc                 <  1:1747792/71‑1 (MQ=255)
                                                    gCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATc                 <  1:884411/71‑1 (MQ=255)
                                                      aGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCAc               <  1:2002269/71‑1 (MQ=255)
                                                        gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                               <  1:3805697/37‑1 (MQ=255)
                                                        gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAggg                    <  1:1973442/64‑1 (MQ=255)
                                                         tACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGc                         <  1:93284/58‑1 (MQ=255)
                                                          aCATGTCGAGATCCAGCGCGTTATGGTGGGTGATaaa                                             <  1:1291034/37‑1 (MQ=255)
                                                          aCATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGa                                         >  1:1211037/1‑41 (MQ=255)
                                                          aCATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCa                                   >  1:182780/1‑47 (MQ=255)
                                                          aCATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCa                       >  1:1299086/1‑59 (MQ=255)
                                                            aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                                           >  1:1346350/1‑37 (MQ=255)
                                                              gTCGAGATACAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATc                 >  1:2050277/1‑61 (MQ=255)
                                                                  agaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCgg   <  1:3994758/71‑1 (MQ=255)
                                                                   gaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                  >  1:353782/1‑39 (MQ=255)
                                                                   gaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGAt                  >  1:1468868/1‑55 (MQ=255)
                                                                   gaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCAcc              >  1:1717186/1‑59 (MQ=255)
                                                                   gaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGc  >  1:467328/1‑71 (MQ=255)
                                                                   |                                                                      
CGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGC  >  minE/1933682‑1933819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: