| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,927 | G→C | 9.1% | L172L (CTC→CTG) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,927 | 0 | G | C | 9.1% | 159.1 / 6.1 | 55 | L172L (CTC→CTG) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (23/27); new base C (3/2); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01 | |||||||||||
GCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGT > minE/1933858‑1933996 | gCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCg < 1:2385275/70‑1 (MQ=255) aTACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATa < 1:1754711/71‑1 (MQ=255) tACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATaa < 1:2259530/71‑1 (MQ=255) tACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATaa < 1:1600095/71‑1 (MQ=255) ccAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCgaga > 1:1411995/1‑66 (MQ=255) ccAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACg > 1:460010/1‑71 (MQ=255) ggATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGa < 1:3847390/71‑1 (MQ=255) tCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCga < 1:2194199/54‑1 (MQ=255) tGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCgaga > 1:3227490/1‑54 (MQ=255) tGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGa < 1:719903/63‑1 (MQ=255) gCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgcgc > 1:4098853/1‑71 (MQ=255) gCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgcgc > 1:2401052/1‑71 (MQ=255) gCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgcg > 1:2299453/1‑70 (MQ=255) cACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGAc < 1:3576545/56‑1 (MQ=255) cACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCt < 1:1039876/71‑1 (MQ=255) tttAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATaa < 1:3074317/46‑1 (MQ=255) tttAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgcgcgc > 1:1055040/1‑63 (MQ=255) ttAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGAt < 1:751647/43‑1 (MQ=255) tAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCgag > 1:1707386/1‑40 (MQ=255) tAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCgag > 1:1172067/1‑40 (MQ=255) tAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGa > 1:2789213/1‑50 (MQ=255) tAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCt > 1:656168/1‑66 (MQ=255) aaaGGTGTTGCGGGATTCATCGTTGGAGATGAGATCgag > 1:1351284/1‑39 (MQ=255) aaaGGTGTTGCGGGATTCATCGTTGGAGATGAGATCgag > 1:3440667/1‑39 (MQ=255) aaGGTGTTGCGGGATTCATCGTTGGAGATGAGATCgag > 1:2322251/1‑38 (MQ=255) aGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGc < 1:3104870/71‑1 (MQ=255) aGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGc < 1:3453558/71‑1 (MQ=255) aGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGc < 1:735565/71‑1 (MQ=255) ttGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACg < 1:1639053/44‑1 (MQ=255) ttGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACg < 1:860012/44‑1 (MQ=255) ttGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCt > 1:2727446/1‑58 (MQ=255) gCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGAt < 1:1261431/44‑1 (MQ=255) gCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgcg > 1:2007165/1‑48 (MQ=255) ggATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTgg < 1:3509917/70‑1 (MQ=255) gATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGaa < 1:1842658/71‑1 (MQ=255) aTTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAt > 1:352920/1‑71 (MQ=255) tttaTCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAAtt > 1:395179/4‑71 (MQ=255) ttCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCt > 1:3150550/1‑50 (MQ=255) tCATCGTTCGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAtt > 1:3280268/1‑70 (MQ=255) taTCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAAttt < 1:1860825/69‑1 (MQ=255) taTCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAAttt < 1:2011318/69‑1 (MQ=255) cATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAttt > 1:3001961/1‑70 (MQ=255) cATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAttt < 1:2690654/70‑1 (MQ=255) aTCATTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCt > 1:2223781/1‑47 (MQ=255) cGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATcc > 1:571348/1‑71 (MQ=255) ttGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGa < 1:878876/61‑1 (MQ=255) ttGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCgg < 1:3810504/71‑1 (MQ=255) ggAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGa > 1:307841/1‑43 (MQ=255) gagatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATc < 1:1448303/44‑1 (MQ=255) agatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGc > 1:2199005/1‑71 (MQ=255) gatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg < 1:2532192/71‑1 (MQ=255) gatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg < 1:616547/71‑1 (MQ=255) tgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGa > 1:3823945/1‑71 (MQ=255) gaggtCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCa > 1:370217/1‑44 (MQ=255) gaggtCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCa > 1:365271/1‑44 (MQ=255) gagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGa > 1:3702366/1‑70 (MQ=255) gagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGAc > 1:3210298/1‑71 (MQ=255) agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTa < 1:3750292/57‑1 (MQ=255) agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGAc < 1:385157/70‑1 (MQ=255) agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGAc < 1:1913520/70‑1 (MQ=255) atCGAGATAACGCTGACGATTGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGc < 1:656722/61‑1 (MQ=255) atCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACg > 1:3037702/1‑69 (MQ=255) cGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGt > 1:1552525/1‑71 (MQ=255) cGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGt > 1:2960645/1‑71 (MQ=255) | GCGAATACCAGAGAGGATCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGT > minE/1933858‑1933996 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |