| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,969 | G→A | 15.8% | H158H (CAC→CAT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,969 | 0 | G | A | 15.8% | 194.8 / 24.6 | 76 | H158H (CAC→CAT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (34/30); new base A (5/7); total (39/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 | |||||||||||
GGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACA > minE/1933903‑1934036 | ggATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTgg < 1:3509917/70‑1 (MQ=255) gATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGaa < 1:1842658/71‑1 (MQ=255) aTTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAt > 1:352920/1‑71 (MQ=255) tttaTCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAAtt > 1:395179/4‑71 (MQ=255) tCATCGTTCGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAtt > 1:3280268/1‑70 (MQ=255) taTCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAAttt < 1:2011318/69‑1 (MQ=255) taTCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAAttt < 1:1860825/69‑1 (MQ=255) cATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAttt < 1:2690654/70‑1 (MQ=255) cATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAttt > 1:3001961/1‑70 (MQ=255) cGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATcc > 1:571348/1‑71 (MQ=255) ttGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGa < 1:878876/61‑1 (MQ=255) ttGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCgg < 1:3810504/71‑1 (MQ=255) agatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGc > 1:2199005/1‑71 (MQ=255) gatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg < 1:2532192/71‑1 (MQ=255) gatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg < 1:616547/71‑1 (MQ=255) tgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGa > 1:3823945/1‑71 (MQ=255) gagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGa > 1:3702366/1‑70 (MQ=255) gagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGAc > 1:3210298/1‑71 (MQ=255) agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTa < 1:3750292/57‑1 (MQ=255) agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGAc < 1:385157/70‑1 (MQ=255) agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGAc < 1:1913520/70‑1 (MQ=255) atCGAGATAACGCTGACGATTGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGc < 1:656722/61‑1 (MQ=255) atCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACg > 1:3037702/1‑69 (MQ=255) cGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGt > 1:2960645/1‑71 (MQ=255) cGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGt > 1:1552525/1‑71 (MQ=255) cagaTAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTg < 1:230532/70‑1 (MQ=255) gaTAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGa < 1:669919/45‑1 (MQ=255) aTAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAtt < 1:2560908/47‑1 (MQ=255) tAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAttt < 1:2460660/47‑1 (MQ=255) tAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCtt < 1:1059104/70‑1 (MQ=255) aaCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTg > 1:796552/1‑71 (MQ=255) aCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCtttag > 1:3589246/1‑69 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGaa < 1:1133831/42‑1 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGaa < 1:227987/42‑1 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGa > 1:3483104/1‑41 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAt > 1:1691153/1‑43 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAAt > 1:3235777/1‑43 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTgg > 1:314281/1‑71 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTg > 1:4118009/1‑70 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTg < 1:2707588/70‑1 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTg < 1:3746688/70‑1 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTg > 1:3009342/1‑70 (MQ=255) gttGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTc < 1:3419323/69‑1 (MQ=255) gCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTc < 1:894359/71‑1 (MQ=255) gtGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTc < 1:650374/69‑1 (MQ=255) cTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTc < 1:169050/70‑1 (MQ=255) tGACGATAGCGCGCTTACTGATCCTGCAAGCCGTGGAATTTATGCgg > 1:2703058/1‑47 (MQ=255) gACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag < 1:2757850/70‑1 (MQ=255) aCGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag < 1:1855939/69‑1 (MQ=255) aCGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag < 1:89432/69‑1 (MQ=255) aCGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag < 1:1107875/69‑1 (MQ=255) aCGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag < 1:1797788/69‑1 (MQ=255) gATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGc < 1:2411612/53‑1 (MQ=255) gATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGa < 1:2087235/71‑1 (MQ=255) tAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACg > 1:3971513/1‑71 (MQ=255) aGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAg > 1:556061/1‑43 (MQ=255) gcgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGc < 1:2648389/70‑1 (MQ=255) gcgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGc < 1:3533304/70‑1 (MQ=255) tCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCa > 1:3720702/1‑43 (MQ=255) tCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCa > 1:1409043/1‑43 (MQ=255) ccTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTca > 1:3455490/1‑67 (MQ=255) ccTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGta > 1:2914750/1‑70 (MQ=255) ccTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGta > 1:1296557/1‑70 (MQ=255) ccTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTg > 1:1651608/1‑71 (MQ=255) tGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTg > 1:1354753/1‑69 (MQ=255) ccTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGCCGCAACTCGGTGCAGTg > 1:427977/1‑70 (MQ=255) ccTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTg > 1:2293852/1‑70 (MQ=255) ccTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTCCAGTg > 1:2777200/1‑70 (MQ=255) gCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGt > 1:914479/1‑61 (MQ=255) gCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTg < 1:101635/67‑1 (MQ=255) gCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGa > 1:1457159/1‑69 (MQ=255) cAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGa > 1:1398104/1‑49 (MQ=255) cAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGa > 1:1697902/1‑68 (MQ=255) gCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCaa > 1:1743731/1‑51 (MQ=255) gCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCaa > 1:2685907/1‑51 (MQ=255) gCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACa < 1:530084/71‑1 (MQ=255) | GGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACA > minE/1933903‑1934036 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |