| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,981 | C→T | 10.9% | P154P (CCG→CCA) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,981 | 0 | C | T | 10.9% | 214.2 / 13.7 | 73 | P154P (CCG→CCA) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (38/27); new base T (4/4); total (42/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01 | |||||||||||
CGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTT > minE/1933911‑1934047 | cGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATcc > 1:571348/1‑71 (MQ=255) ttGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCgg < 1:3810504/71‑1 (MQ=255) agatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGc > 1:2199005/1‑71 (MQ=255) gatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg < 1:2532192/71‑1 (MQ=255) gatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg < 1:616547/71‑1 (MQ=255) tgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGa > 1:3823945/1‑71 (MQ=255) gagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGa > 1:3702366/1‑70 (MQ=255) gagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGAc > 1:3210298/1‑71 (MQ=255) agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGAc < 1:385157/70‑1 (MQ=255) agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGAc < 1:1913520/70‑1 (MQ=255) atCGAGATAACGCTGACGATTGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGc < 1:656722/61‑1 (MQ=255) atCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACg > 1:3037702/1‑69 (MQ=255) cGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGt > 1:2960645/1‑71 (MQ=255) cGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGt > 1:1552525/1‑71 (MQ=255) cagaTAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTg < 1:230532/70‑1 (MQ=255) tAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCtt < 1:1059104/70‑1 (MQ=255) aaCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTg > 1:796552/1‑71 (MQ=255) aCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCtttag > 1:3589246/1‑69 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTgg > 1:314281/1‑71 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTg < 1:3746688/70‑1 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTg < 1:2707588/70‑1 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTg > 1:4118009/1‑70 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTg > 1:3009342/1‑70 (MQ=255) gttGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTc < 1:3419323/69‑1 (MQ=255) gCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTc < 1:894359/71‑1 (MQ=255) gtGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTc < 1:650374/69‑1 (MQ=255) cTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTc < 1:169050/70‑1 (MQ=255) tGACGATAGCGCGCTTACTGATCCTGCAAGCCGTGGAATTTATGCgg > 1:2703058/1‑47 (MQ=255) gACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag < 1:2757850/70‑1 (MQ=255) aCGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag < 1:1855939/69‑1 (MQ=255) aCGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag < 1:1107875/69‑1 (MQ=255) aCGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag < 1:1797788/69‑1 (MQ=255) aCGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcag < 1:89432/69‑1 (MQ=255) gATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGc < 1:2411612/53‑1 (MQ=255) gATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGa < 1:2087235/71‑1 (MQ=255) tAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACg > 1:3971513/1‑71 (MQ=255) aGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAg > 1:556061/1‑43 (MQ=255) gcgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGc < 1:3533304/70‑1 (MQ=255) gcgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGc < 1:2648389/70‑1 (MQ=255) tCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCa > 1:3720702/1‑43 (MQ=255) tCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCa > 1:1409043/1‑43 (MQ=255) ccTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTca > 1:3455490/1‑67 (MQ=255) ccTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGta > 1:2914750/1‑70 (MQ=255) ccTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGta > 1:1296557/1‑70 (MQ=255) ccTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTg > 1:1651608/1‑71 (MQ=255) tGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTg > 1:1354753/1‑69 (MQ=255) ccTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGCCGCAACTCGGTGCAGTg > 1:427977/1‑70 (MQ=255) ccTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTg > 1:2293852/1‑70 (MQ=255) ccTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTCCAGTg > 1:2777200/1‑70 (MQ=255) gCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGt > 1:914479/1‑61 (MQ=255) gCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTg < 1:101635/67‑1 (MQ=255) gCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGa > 1:1457159/1‑69 (MQ=255) cAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGa > 1:1398104/1‑49 (MQ=255) cAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGa > 1:1697902/1‑68 (MQ=255) gCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCaa > 1:2685907/1‑51 (MQ=255) gCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCaa > 1:1743731/1‑51 (MQ=255) gCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACa < 1:530084/71‑1 (MQ=255) tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTgg < 1:3344496/60‑1 (MQ=255) tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTc > 1:2591501/1‑71 (MQ=255) tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTc < 1:3086584/71‑1 (MQ=255) ggAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAAc > 1:162055/1‑47 (MQ=255) gAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa < 1:1069585/63‑1 (MQ=255) gAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa > 1:2257452/1‑63 (MQ=255) gAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCg > 1:460989/1‑70 (MQ=255) aTTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGc < 1:4027176/41‑1 (MQ=255) aTTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGa > 1:2629058/1‑57 (MQ=255) aTTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGa > 1:1534944/1‑57 (MQ=255) tttATCCGGCAGCGGACGCAGTGCTTTGGTcagca < 1:2718200/35‑1 (MQ=255) tttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGc < 1:2526827/50‑1 (MQ=255) ttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCaa > 1:579549/1‑41 (MQ=255) tATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGa > 1:1983854/1‑35 (MQ=255) tATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGa > 1:3563845/1‑35 (MQ=255) tATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggtt > 1:1964796/1‑71 (MQ=255) tATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggtt > 1:3514143/1‑71 (MQ=255) | CGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTT > minE/1933911‑1934047 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |