| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 310,791 | T→G | 7.4% | K694N (AAA→AAC) ‡ | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 310,791 | 0 | T | G | 7.4% | 134.7 / 5.4 | 54 | K694N (AAA→AAC) ‡ | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (31/19); new base G (2/2); total (33/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCAC > minE/310721‑310859 | gACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGt < 1:2774767/71‑1 (MQ=255) tACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGttttt > 1:3404459/1‑71 (MQ=255) gCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt < 1:3881213/71‑1 (MQ=255) gCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt < 1:1314718/71‑1 (MQ=255) tGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGa > 1:1589900/1‑71 (MQ=255) cAACATATCAGGGTTCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc > 1:1135521/1‑69 (MQ=255) aaCATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAg > 1:3249148/1‑71 (MQ=255) aaCATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAg > 1:2546251/1‑71 (MQ=255) tatCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGt < 1:3054777/53‑1 (MQ=255) tCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGc > 1:1691799/1‑71 (MQ=255) gggTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTg < 1:1904122/71‑1 (MQ=255) gTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGt > 1:453074/1‑71 (MQ=255) cTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCaa > 1:1298517/1‑71 (MQ=255) tCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt > 1:1500400/1‑70 (MQ=255) tCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTCACCAAGGCCAGCCTGGTCAAt > 1:1631682/1‑70 (MQ=255) cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCaa > 1:312101/1‑53 (MQ=255) cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt > 1:1303049/1‑69 (MQ=255) gctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCa < 1:1218891/70‑1 (MQ=255) ctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCa > 1:1615559/1‑69 (MQ=255) gctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt > 1:2075878/1‑70 (MQ=255) ctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGc > 1:468456/1‑55 (MQ=255) ctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTc < 1:377604/61‑1 (MQ=255) ctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca < 1:498104/71‑1 (MQ=255) ctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca < 1:3699435/71‑1 (MQ=255) ctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtc > 1:4019568/1‑70 (MQ=255) tCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca > 1:2032543/1‑69 (MQ=255) gcaagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagt > 1:3668354/1‑70 (MQ=255) gcaagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCAAGCCTGGTCAATCAGCtcaaagt > 1:2797037/1‑70 (MQ=255) ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaa > 1:476855/2‑68 (MQ=255) ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaa > 1:1111326/2‑68 (MQ=255) caagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagt < 1:3593032/69‑1 (MQ=255) aagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcga < 1:3619062/71‑3 (MQ=255) aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca > 1:2451158/1‑70 (MQ=255) gcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCTATTAACtcaaa < 1:2812089/64‑1 (MQ=255) gcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag > 1:3793891/1‑71 (MQ=255) caaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGc > 1:28976/1‑71 (MQ=255) aaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc > 1:976190/1‑71 (MQ=255) cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCa < 1:1189699/49‑1 (MQ=255) cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:3286078/69‑1 (MQ=255) tGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGt > 1:4017314/1‑71 (MQ=255) ggTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATc > 1:823528/1‑50 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGt < 1:2653576/69‑1 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg < 1:1415522/71‑1 (MQ=255) ttACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCATCCTGGTCAATCAGCTCAAAGTCAAAGCGGGTTg < 1:1118833/70‑1 (MQ=255) ttACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg > 1:817641/1‑70 (MQ=255) aCGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCt > 1:2326921/1‑38 (MQ=255) aCGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCt > 1:2039995/1‑38 (MQ=255) cgcgCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg < 1:263900/67‑1 (MQ=255) cgcgCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAg < 1:2561521/70‑1 (MQ=255) cgcgCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAg < 1:2273834/70‑1 (MQ=255) aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGtt > 1:985914/1‑71 (MQ=255) gTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTc > 1:1171458/1‑71 (MQ=255) tgaGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTc > 1:2442801/3‑70 (MQ=255) tCAGTTTTTCGTGACCAAGGCCGGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:2581810/52‑1 (MQ=255) tCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTcc < 1:2049929/71‑1 (MQ=255) aGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCa < 1:3308177/70‑1 (MQ=255) aGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCAc > 1:3860641/1‑71 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa < 1:930832/38‑1 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCg < 1:2477937/49‑1 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCa > 1:24111/1‑68 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCAc > 1:2252873/1‑69 (MQ=255) | GACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCAC > minE/310721‑310859 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |