| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,934,200 | A→C | 7.5% | G81G (GGT→GGG) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,934,200 | 0 | A | C | 7.5% | 195.2 / 12.2 | 80 | G81G (GGT→GGG) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (41/33); new base C (4/2); total (45/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
GATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCG > minE/1934133‑1934269 | gaTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc < 1:2479070/71‑1 (MQ=255) aTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCa < 1:2834432/71‑1 (MQ=255) cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc > 1:1134474/1‑68 (MQ=255) cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:1659434/1‑70 (MQ=255) cGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat > 1:1590/1‑70 (MQ=255) gTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:3335416/1‑71 (MQ=255) gTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa < 1:3227327/71‑1 (MQ=255) cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataata > 1:796881/1‑71 (MQ=255) cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataata > 1:2071619/1‑71 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac < 1:2619889/71‑1 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:1131024/1‑71 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac < 1:1245107/71‑1 (MQ=255) gTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtca > 1:2599136/1‑71 (MQ=255) tACAGTTGAATACGGCCACCGCCATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGtcat > 1:2804638/1‑71 (MQ=255) tACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat < 1:3061527/71‑1 (MQ=255) tACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat > 1:1659106/1‑71 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatca > 1:845002/1‑71 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:2641620/70‑1 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:4006380/70‑1 (MQ=255) cTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat < 1:3360401/66‑1 (MQ=255) gAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCgg > 1:3540836/1‑71 (MQ=255) aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac < 1:1345227/53‑1 (MQ=255) aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac < 1:3485321/53‑1 (MQ=255) aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGc > 1:3049821/1‑71 (MQ=255) aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGc > 1:514788/1‑71 (MQ=255) aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGc > 1:2237271/1‑71 (MQ=255) cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:3091539/1‑70 (MQ=255) cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc < 1:920082/71‑1 (MQ=255) cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc < 1:2561469/71‑1 (MQ=255) ccGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAAc < 1:1713652/71‑1 (MQ=255) ccAACGTCATGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc > 1:4095849/1‑71 (MQ=255) aaCGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgac < 1:3904713/44‑1 (MQ=255) gTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGcc > 1:817834/1‑57 (MQ=255) gTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACtt > 1:1098166/1‑71 (MQ=255) atCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCaa > 1:2277651/2‑62 (MQ=255) atCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCaa > 1:109108/2‑62 (MQ=255) tCCTGCAGGGTAACGAAAGAGGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc < 1:3985191/59‑1 (MQ=255) tCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGc < 1:32884/52‑1 (MQ=255) tCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:3040834/1‑61 (MQ=255) tCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACttg < 1:1051536/71‑2 (MQ=255) tCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTc < 1:2166119/71‑1 (MQ=255) tCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTc < 1:437585/71‑1 (MQ=255) tCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTc < 1:1555851/71‑1 (MQ=255) ccTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc > 1:630386/1‑47 (MQ=255) ccTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTcatcat < 1:1681746/49‑1 (MQ=255) cTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc > 1:1301552/1‑46 (MQ=255) cTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa > 1:1566708/1‑71 (MQ=255) cTGCAGGGTAACGAAAGACGCTTTACCCATAAGACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa > 1:3726255/1‑71 (MQ=255) cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTcaa > 1:636344/1‑69 (MQ=255) tGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATg < 1:3934474/48‑1 (MQ=255) cAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatca > 1:42577/1‑44 (MQ=255) ggTAACGAAAGACGCTTTACCCATAATACGACGGGt > 1:1097528/1‑36 (MQ=255) gTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCa > 1:1977437/1‑70 (MQ=255) tAACGAAAGACGCTTTACCCATAATACGACGGGtcatca > 1:838127/1‑39 (MQ=255) tAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATg < 1:857792/65‑1 (MQ=255) aCGAAAGACGCTTTACCCATAATACGACGGGtcatca > 1:1197443/1‑37 (MQ=255) aCGAAAGACGCTTTACCCATAATACGACGGGtcatca > 1:1285005/1‑37 (MQ=255) aCGAAAGACGCTTTACCCATAATACGACGGGtcatca > 1:2340240/1‑37 (MQ=255) aCGAAAGACGCTTTACCCATAATACGACGGGtcatca > 1:421539/1‑37 (MQ=255) aCGAAAGACGCTTTACCCATAATACGACGGGtcaaca > 1:2920863/1‑37 (MQ=255) aCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa > 1:3775650/1‑61 (MQ=255) aCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt < 1:281911/65‑1 (MQ=255) aaaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc < 1:2268438/44‑1 (MQ=255) aaaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc < 1:1108702/71‑1 (MQ=255) aaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa < 1:3446168/45‑1 (MQ=255) aaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACtt > 1:1983487/1‑54 (MQ=255) aaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTcc < 1:2172686/71‑1 (MQ=255) aGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc > 1:1663461/1‑69 (MQ=255) gACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAg < 1:1960104/71‑1 (MQ=255) aCGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAg > 1:840469/1‑61 (MQ=255) aCGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGt > 1:600392/1‑71 (MQ=255) cGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGtt < 1:3470108/71‑1 (MQ=255) cGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGtt < 1:2684230/71‑1 (MQ=255) cTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATg < 1:779022/53‑1 (MQ=255) tttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt < 1:100945/54‑1 (MQ=255) tttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttctt > 1:1023094/1‑71 (MQ=255) ttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa > 1:2187415/1‑49 (MQ=255) ttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt > 1:2649902/1‑53 (MQ=255) ttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc < 1:2620888/62‑1 (MQ=255) ttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttcttc > 1:2543666/1‑71 (MQ=255) aCCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg < 1:1130741/70‑1 (MQ=255) | GATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCG > minE/1934133‑1934269 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |