| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,934,224 | G→T | 10.5% | R73R (CGC→CGA) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,934,224 | 0 | G | T | 10.5% | 274.7 / 11.5 | 85 | R73R (CGC→CGA) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (41/35); new base T (6/3); total (47/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGT > minE/1934157‑1934294 | gAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCgg > 1:3540836/1‑71 (MQ=255) aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGc > 1:2237271/1‑71 (MQ=255) aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGc > 1:514788/1‑71 (MQ=255) aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGc > 1:3049821/1‑71 (MQ=255) cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:3091539/1‑70 (MQ=255) cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc < 1:2561469/71‑1 (MQ=255) cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc < 1:920082/71‑1 (MQ=255) ccGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAAc < 1:1713652/71‑1 (MQ=255) ccAACGTCATGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc > 1:4095849/1‑71 (MQ=255) gTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGcc > 1:817834/1‑57 (MQ=255) gTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACtt > 1:1098166/1‑71 (MQ=255) atCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCaa > 1:109108/2‑62 (MQ=255) atCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCaa > 1:2277651/2‑62 (MQ=255) tCCTGCAGGGTAACGAAAGAGGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc < 1:3985191/59‑1 (MQ=255) tCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGc < 1:32884/52‑1 (MQ=255) tCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:3040834/1‑61 (MQ=255) tCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACttg < 1:1051536/71‑2 (MQ=255) tCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTc < 1:2166119/71‑1 (MQ=255) tCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTc < 1:437585/71‑1 (MQ=255) tCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTc < 1:1555851/71‑1 (MQ=255) cTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa > 1:1566708/1‑71 (MQ=255) cTGCAGGGTAACGAAAGACGCTTTACCCATAAGACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa > 1:3726255/1‑71 (MQ=255) cTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTcaa > 1:636344/1‑69 (MQ=255) tGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATg < 1:3934474/48‑1 (MQ=255) gTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCa > 1:1977437/1‑70 (MQ=255) tAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATg < 1:857792/65‑1 (MQ=255) aCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa > 1:3775650/1‑61 (MQ=255) aCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt < 1:281911/65‑1 (MQ=255) aaaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc < 1:2268438/44‑1 (MQ=255) aaaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc < 1:1108702/71‑1 (MQ=255) aaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa < 1:3446168/45‑1 (MQ=255) aaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACtt > 1:1983487/1‑54 (MQ=255) aaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTcc < 1:2172686/71‑1 (MQ=255) aGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc > 1:1663461/1‑69 (MQ=255) gACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAg < 1:1960104/71‑1 (MQ=255) aCGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAg > 1:840469/1‑61 (MQ=255) aCGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGt > 1:600392/1‑71 (MQ=255) cGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGtt < 1:3470108/71‑1 (MQ=255) cGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGtt < 1:2684230/71‑1 (MQ=255) cTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATg < 1:779022/53‑1 (MQ=255) tttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt < 1:100945/54‑1 (MQ=255) tttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttctt > 1:1023094/1‑71 (MQ=255) ttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa > 1:2187415/1‑49 (MQ=255) ttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt > 1:2649902/1‑53 (MQ=255) ttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc < 1:2620888/62‑1 (MQ=255) ttACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttcttc > 1:2543666/1‑71 (MQ=255) aCCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg < 1:1130741/70‑1 (MQ=255) cccATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg < 1:908885/69‑1 (MQ=255) cATGATACGACGGGTCATCATTCGGCCAGCAACCTAGACTTCAATGTTTAAGGATTCCAGTTCCTGGttat > 1:3574618/1‑69 (MQ=255) cATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtct < 1:3419247/71‑1 (MQ=255) ataataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAgcg > 1:2465860/1‑52 (MQ=255) ataataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtctc > 1:2254108/1‑71 (MQ=255) tgataCGACGGGTCATCATTCGGCCAGCAACCGAGACTTc < 1:3355678/38‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATg < 1:1301943/44‑1 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt > 1:3156672/1‑71 (MQ=255) ataCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCctt > 1:515733/1‑71 (MQ=255) ataCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCctt > 1:1575533/1‑71 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCg > 1:3043812/1‑39 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt < 1:1308797/71‑1 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:3368729/1‑71 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:2479681/1‑71 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTT‑‑AGTTCTTCGTTCTCTTTGc > 1:1943096/1‑71 (MQ=255) gacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCg > 1:1101812/1‑71 (MQ=255) acgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGt < 1:1299553/71‑1 (MQ=255) cgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt < 1:1606635/59‑1 (MQ=255) cgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTc < 1:1364761/71‑1 (MQ=255) gggTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCg < 1:2595525/71‑1 (MQ=255) gctcatcaTGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc < 1:1176970/43‑1 (MQ=255) gTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa < 1:3162616/71‑1 (MQ=255) catcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAtt > 1:3651078/1‑71 (MQ=255) atcatGCGGCCAGCAACGGCGACTTCGATGTTCAg > 1:2355558/1‑35 (MQ=255) tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt < 1:2365464/52‑1 (MQ=255) tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt < 1:718834/52‑1 (MQ=255) tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTc > 1:288390/1‑70 (MQ=255) tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCt < 1:3803520/71‑1 (MQ=255) tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCt < 1:3993051/71‑1 (MQ=255) catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa > 1:2687599/1‑66 (MQ=255) catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg > 1:1345016/1‑71 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTcc > 1:4091039/1‑39 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCa > 1:1421734/1‑40 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtct > 1:1736851/1‑52 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCg < 1:2176049/63‑1 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGa > 1:2994118/1‑64 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGa > 1:2482577/1‑64 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGc > 1:15203/1‑71 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGc > 1:1994772/1‑71 (MQ=255) gCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc > 1:1256312/1‑36 (MQ=255) gCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGt > 1:38936/1‑71 (MQ=255) | GAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGT > minE/1934157‑1934294 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |