| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,948,347 | C→A | 16.2% | intergenic (‑38/+81) | gcvP ← / ← gcvH | glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex/glycine cleavage complex lipoylprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,948,347 | 0 | C | A | 16.2% | 261.8 / 22.6 | 86 | intergenic (‑38/+81) | gcvP/gcvH | glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex/glycine cleavage complex lipoylprotein |
| Reads supporting (aligned to +/- strand): ref base C (31/41); new base A (9/5); total (40/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.62e-01 | |||||||||||
TTTTCAAGCTGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTCACCCTGATCCTCTCCCGCAGAAGAG > minE/1948281‑1948416 | ttttCAAGCTGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACctctc > 1:3841196/1‑71 (MQ=255) tttCAAGCTGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACctctct > 1:3704325/1‑71 (MQ=255) tttCAAGCTGGCTTAACGTCTGTGTCATGAGCGATGGTTCATGAAACGTGCAGTGAATTGTGAACatctc < 1:2241299/70‑1 (MQ=255) ttCAAGCTGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACctctct < 1:3546378/70‑1 (MQ=255) ttCAAGCTGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACctctct > 1:2145737/1‑70 (MQ=255) ttCAAGCTGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCtctctc > 1:3705117/1‑71 (MQ=255) ttCAAGCTGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCtctctc > 1:169746/1‑71 (MQ=255) ttCAAGCTGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCtctctc > 1:3198929/1‑71 (MQ=255) aGCTGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAAcc < 1:601852/61‑1 (MQ=255) gCTGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACATCTCTCCTTAc > 1:2091168/1‑71 (MQ=255) cTGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACg < 1:3149787/71‑1 (MQ=255) cTGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACATCTCTCCTTAc > 1:1442453/1‑70 (MQ=255) cTGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACATCTCTCCTTAc > 1:1489819/1‑70 (MQ=255) cTGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACATCTCTCCTTACg < 1:3819649/71‑1 (MQ=255) tGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACctct > 1:2929598/1‑61 (MQ=255) tGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACg > 1:3524428/1‑70 (MQ=255) tGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACg > 1:3682409/1‑70 (MQ=255) tGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACg > 1:3667129/1‑70 (MQ=255) ggCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACctct > 1:3327687/1‑60 (MQ=255) gCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGa < 1:2228151/69‑1 (MQ=255) ttAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGaa > 1:357743/1‑68 (MQ=255) aaCGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACctctct > 1:1833398/1‑57 (MQ=255) aaCGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAagagag > 1:1294802/1‑71 (MQ=255) aCGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAagagag < 1:2400177/70‑1 (MQ=255) aCGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAagagag < 1:2649869/70‑1 (MQ=255) aCGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgt < 1:90512/71‑1 (MQ=255) cGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgtg < 1:515016/71‑1 (MQ=255) cGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgt > 1:3010805/1‑70 (MQ=255) cGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgt > 1:907047/1‑70 (MQ=255) cGTCTGTGTCATGAACGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgt > 1:988548/1‑70 (MQ=255) gTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgtga < 1:2119530/71‑1 (MQ=255) gTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgtga < 1:3454517/71‑1 (MQ=255) gTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACATCTCTCCTTACGAAGAGAgt > 1:1336981/1‑69 (MQ=255) tctgtCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgtgagg < 1:2307603/68‑1 (MQ=255) gtgtCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgtgag < 1:2363651/68‑1 (MQ=255) tgtCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgtgagggt < 1:2384251/70‑1 (MQ=255) tgtCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgtgagggt < 1:290045/70‑1 (MQ=255) tgtCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgtgaggg < 1:1418421/69‑1 (MQ=255) tgtCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACATCTCTCCTTACGAAGAGAgtgagggt > 1:2105031/1‑70 (MQ=255) tgtCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACATCTCTCCTTACGAAGAGAgtgagggt < 1:3958688/70‑1 (MQ=255) tgtCATGAGCGATGGATCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgtgagggt < 1:1630481/70‑1 (MQ=255) gtCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgtgagggt > 1:398845/1‑69 (MQ=255) gtCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgtgaggg > 1:1461313/1‑68 (MQ=255) aTGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTTAGGc < 1:4093485/71‑1 (MQ=255) tGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCt < 1:3207411/47‑1 (MQ=255) gAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCa > 1:1631350/1‑70 (MQ=255) aGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTcc > 1:2612596/1‑44 (MQ=255) aGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAagag > 1:1106258/1‑54 (MQ=255) aGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgtgagggtgag > 1:31944/1‑66 (MQ=255) aGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgtgag > 1:339373/1‑60 (MQ=255) aGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCAt > 1:1529256/1‑70 (MQ=255) aGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCAt > 1:18876/1‑70 (MQ=255) gCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGaa < 1:2853123/50‑1 (MQ=255) gCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACATCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATa > 1:1679191/1‑70 (MQ=255) cGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAagag < 1:3330272/52‑1 (MQ=255) cGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGTTGAGGCATaaa < 1:3107923/71‑1 (MQ=255) aTGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgtgagggt < 1:2166550/59‑1 (MQ=255) tGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgtgagggtga > 1:3142303/1‑60 (MQ=255) tGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAAttt < 1:2486855/71‑1 (MQ=255) ggTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAAtttt > 1:1668607/1‑71 (MQ=255) ggTTCCTGAAACGTGCAGTGAATTGTGAACATCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAAtttt > 1:2279561/1‑71 (MQ=255) gTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGa < 1:1059765/43‑1 (MQ=255) gTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGc > 1:3872905/1‑61 (MQ=255) ttCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAag < 1:2769486/44‑1 (MQ=255) ttCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTcc < 1:2374134/71‑1 (MQ=255) ttCCTGAAACGTGCAGTGAATTGTGAACATCTCTCCTTACGAaga < 1:2574576/45‑1 (MQ=255) tCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGaa > 1:1139357/1‑42 (MQ=255) tCCTGAAACGTGCAGTGAATTGTGAACATCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCt > 1:760001/1‑71 (MQ=255) ccTTAAACGTGCAGTGAATTGTTAACCTCTCTCCTTAAGaa < 1:3696254/41‑1 (MQ=37) ccTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCt < 1:423090/70‑1 (MQ=255) cTGAAACGTGCAGTGAATTGTGAACATCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTc < 1:2559798/70‑1 (MQ=255) cTGAAACGTGCAGTGAATTGTGAACATCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTc < 1:3054796/70‑1 (MQ=255) tGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAagag < 1:414163/42‑1 (MQ=255) aaaCGTGCAGTGAATTGTGAACCTCTCTCCTTACGaa < 1:1661919/37‑1 (MQ=255) aaaCGTGCAGTGAATTGTGAACCTCTCTCCTTACGaa < 1:2748026/37‑1 (MQ=255) aaaCGTGCAGTGAAGTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTCAcc < 1:4036963/70‑1 (MQ=255) aaCGTGCAGTGAATTGTGAACCTCTCTCCTTACGaa < 1:1388214/36‑1 (MQ=255) aaCGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTCAcc < 1:2798044/69‑1 (MQ=255) aCGTGCAGTGAATTGTGAACCTCTCTCCTTACGAaga > 1:3299910/1‑37 (MQ=255) cGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTCACCCTGa < 1:3746288/71‑1 (MQ=255) cAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAgtga < 1:2207257/38‑1 (MQ=255) gTGAATTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATaa < 1:4053705/49‑1 (MQ=255) aaTTGTGAACCTCTCTCCTTACGAAGAGAgtgaggg > 1:388102/1‑36 (MQ=255) aaTTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAAtttt > 1:2845152/1‑51 (MQ=255) aaTTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTCACCCTGATCCTCTccc > 1:1949101/1‑71 (MQ=255) ttGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTCACCCTTATCCTCTCCCg > 1:3867417/1‑70 (MQ=255) gtgAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTCAcc < 1:3204371/54‑1 (MQ=255) gtgAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTCACCCTGATCCTCTCCCg > 1:2433118/1‑68 (MQ=255) tgAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTCACCCTGATCCTCTCCCGCag < 1:2192582/70‑1 (MQ=255) tgAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTCACCCTGATCCTCTCCCGCag < 1:123836/70‑1 (MQ=255) tgAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTCACCCTGATCCTCTCACGCag < 1:1624438/70‑1 (MQ=255) gAACATCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTCAcc > 1:2876679/1‑52 (MQ=255) aaCCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTCAcc < 1:2831643/51‑1 (MQ=255) aaCCTCTCTCCTTACGAAGAGAGTGAGGGAGAGGCAAAAAtttt > 1:718174/1‑44 (MQ=38) aCCTCTCTCCTTACGAAGAGAGTGAGGGGGAGGCATAAATTTTCCTCAcc < 1:392281/50‑1 (MQ=255) ccTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTCACCCTGATCCTCTCCCGCAGAagag < 1:3844078/71‑1 (MQ=255) ccTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTCACCCTGATCCTCTCCCGCAGAagag > 1:2152216/1‑71 (MQ=255) | TTTTCAAGCTGGCTTAACGTCTGTGTCATGAGCGATGGTTCCTGAAACGTGCAGTGAATTGTGAACCTCTCTCCTTACGAAGAGAGTGAGGGTGAGGCATAAATTTTCCTCACCCTGATCCTCTCCCGCAGAAGAG > minE/1948281‑1948416 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |