| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,016,338 | G→A | 18.9% | D6N (GAT→AAT) | yghB → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,016,338 | 0 | G | A | 18.9% | 223.5 / 41.1 | 95 | D6N (GAT→AAT) | yghB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (38/39); new base A (13/5); total (51/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
ATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTT > minE/2016268‑2016407 | aTTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAg < 1:813050/71‑1 (MQ=255) tCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGa > 1:2906379/1‑70 (MQ=255) gTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatcatc < 1:3797466/71‑1 (MQ=255) cAATAGGCAGATAAAGGCTTAAACGCTGTTCCATAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCt > 1:3686200/1‑71 (MQ=255) cAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGatat > 1:2020821/1‑64 (MQ=255) cAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCt > 1:1416762/1‑71 (MQ=255) aTAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTgc > 1:3609402/1‑71 (MQ=255) tAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTgcg > 1:1143948/1‑71 (MQ=255) tAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTgcg > 1:1290784/1‑71 (MQ=255) ggCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGct < 1:3141166/71‑1 (MQ=255) ggCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGct < 1:2769212/71‑1 (MQ=255) gCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTgcgc < 1:333681/69‑1 (MQ=255) aGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCt > 1:3054020/1‑63 (MQ=255) gATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGa < 1:2435904/52‑1 (MQ=255) gATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGc < 1:2236188/61‑1 (MQ=255) aTAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGGTCTGGc < 1:3500601/71‑1 (MQ=255) aTAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGc < 1:2970574/71‑1 (MQ=255) tAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGc > 1:1783815/1‑59 (MQ=255) tAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGc > 1:3869457/1‑59 (MQ=255) tAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGc > 1:2666488/1‑70 (MQ=255) tAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGc > 1:2326041/1‑70 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGctct > 1:771736/1‑66 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGctct > 1:999354/1‑66 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCaa > 1:952287/1‑71 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCaa > 1:1986867/1‑71 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCaa > 1:518059/1‑71 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCaa > 1:1884048/1‑71 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCaa > 1:3816433/1‑71 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCaa > 1:3081285/1‑71 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCaa > 1:1897425/1‑71 (MQ=255) aaaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCaa > 1:3699759/1‑71 (MQ=255) aaGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCaa < 1:189128/70‑1 (MQ=255) aGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCaa > 1:2865322/1‑69 (MQ=255) gCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAacac > 1:1406251/1‑70 (MQ=255) cTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatc < 1:1252639/48‑1 (MQ=255) cTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatc < 1:2879832/48‑1 (MQ=255) cTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGa < 1:1206360/71‑1 (MQ=255) ttAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGAc > 1:3597890/1‑71 (MQ=255) ttAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGAc > 1:325231/1‑71 (MQ=255) tAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATatcat > 1:2029687/1‑48 (MQ=255) tAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGc > 1:2869517/1‑51 (MQ=255) aaaCGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACtt > 1:1001323/1‑71 (MQ=255) aaaCGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACt < 1:3787397/70‑1 (MQ=255) cGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAAata > 1:2123492/1‑40 (MQ=255) gCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGc < 1:2348940/57‑1 (MQ=255) cTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCcg > 1:2302415/1‑71 (MQ=255) ttCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTgcg < 1:2332726/46‑1 (MQ=255) tCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCt > 1:3501824/1‑71 (MQ=255) tCCACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGc < 1:2513742/52‑1 (MQ=255) tCCACACGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCt > 1:278209/1‑71 (MQ=255) ccACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGctc < 1:3689393/47‑1 (MQ=255) ccACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGctc < 1:3404273/47‑1 (MQ=255) ccACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCt < 1:4087863/70‑1 (MQ=255) ccACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCt < 1:2299391/70‑1 (MQ=255) ccACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCt < 1:621472/70‑1 (MQ=255) ccACAGGAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTg > 1:3932027/1‑71 (MQ=255) cacaGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTgg > 1:1331817/1‑71 (MQ=255) aGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCTCGCTGGCgg < 1:1491682/71‑1 (MQ=255) ggAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAacac < 1:1337952/51‑1 (MQ=255) ggAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCgcgc > 1:3046285/1‑64 (MQ=255) ggAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGa < 1:4079030/71‑1 (MQ=255) ggAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGa < 1:286294/71‑1 (MQ=255) gAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGAt > 1:1958510/1‑71 (MQ=255) gAAAGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCcgc < 1:1280413/61‑1 (MQ=255) aaGTCCATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACACGACtt > 1:954421/1‑53 (MQ=255) aGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTg < 1:2293775/54‑1 (MQ=255) tCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCcgcg < 1:1153182/57‑1 (MQ=255) tCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCcgcg < 1:1539263/57‑1 (MQ=255) tCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTgg < 1:518132/61‑1 (MQ=255) tCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTgg < 1:1818467/61‑1 (MQ=255) tCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCa > 1:3425914/1‑71 (MQ=255) tCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCa < 1:1672462/71‑1 (MQ=255) tCCATGGCTGTTATTCAAAATATCATCGGTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCa < 1:1892940/71‑1 (MQ=255) ccATGGCTGTTATTCAAGATATCATCGCTGCGCTCTgg > 1:3327133/1‑38 (MQ=255) ccATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCa > 1:2222646/1‑40 (MQ=255) cATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCata < 1:3121209/71‑1 (MQ=255) cATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCata < 1:1530913/71‑1 (MQ=255) cATGGCTGTTATTCAAAATATCATCGCTGCGCTCTGGCAACGCGACTTTGCCGCGCTGGCGGATCCTCat > 1:4042837/1‑70 (MQ=255) aTGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGa < 1:3560448/44‑1 (MQ=255) aTGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGa < 1:2280085/44‑1 (MQ=255) aTGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGa < 1:1335919/44‑1 (MQ=255) aTGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGAc < 1:640691/45‑1 (MQ=255) aTGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGAc < 1:1840982/45‑1 (MQ=255) tGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATAtt < 1:332073/71‑1 (MQ=255) gCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGa > 1:521532/1‑41 (MQ=255) gCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGt > 1:2928817/1‑71 (MQ=255) tGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACttt > 1:2868032/1‑43 (MQ=255) tGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACtt > 1:240032/1‑42 (MQ=255) tATTCAAAATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCg > 1:1437301/1‑71 (MQ=255) aTTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGc > 1:998323/1‑41 (MQ=255) aTTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCata > 1:2473215/1‑61 (MQ=255) aTTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATAtt > 1:864845/1‑63 (MQ=255) aTTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgt > 1:500452/1‑71 (MQ=255) aaGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCgttgt < 1:3871331/70‑1 (MQ=255) aGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTTATATTGTTAGCGTTGttt < 1:3943647/71‑1 (MQ=255) aGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGttt < 1:1833176/71‑1 (MQ=255) | ATTCAGGAGTACAATAGGCAGATAAAGGCTTAAACGCTGTTCCACAGGAAAGTCCATGGCTGTTATTCAAGATATCATCGCTGCGCTCTGGCAACACGACTTTGCCGCGCTGGCGGATCCTCATATTGTTAGCGTTGTTT > minE/2016268‑2016407 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |