| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,023,066 | G→A | 21.3% | P281S (CCA→TCA) | ygiQ ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,023,066 | 0 | G | A | 21.3% | 136.3 / 32.3 | 66 | P281S (CCA→TCA) | ygiQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (22/29); new base A (7/7); total (30/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.05e-01 | |||||||||||
TATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATC > minE/2022997‑2023135 | tATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGc > 1:2600894/1‑71 (MQ=255) gCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGc < 1:823952/67‑1 (MQ=255) ccTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACg < 1:3012931/71‑1 (MQ=255) cTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGc < 1:1388442/67‑1 (MQ=255) cACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTa > 1:1352445/1‑71 (MQ=255) cACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGACTGCACGGTTa > 1:2541808/1‑71 (MQ=255) aCTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTAc < 1:1144730/71‑1 (MQ=255) tttCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGACTGCACGGTTACgg > 1:3347261/1‑71 (MQ=255) ttCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTa > 1:1629731/1‑67 (MQ=255) tctcGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGc < 1:3885346/70‑1 (MQ=255) tctcGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCAAGGTTTCGGGCGCGGTGACTGCAc < 1:4055204/61‑1 (MQ=255) ctcGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCtt > 1:2276601/1‑71 (MQ=255) tcGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGACt > 1:3305427/1‑55 (MQ=255) gAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGACTGCACGGTTACGGCttt < 1:1095598/69‑1 (MQ=255) aagaagGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCt < 1:882374/71‑1 (MQ=255) gcaACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCttt < 1:1733426/71‑1 (MQ=255) aaCACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCttttt > 1:2796147/1‑71 (MQ=255) aaCACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCttttt > 1:389415/1‑71 (MQ=255) aaCACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCttttt > 1:1923488/1‑71 (MQ=255) acacGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTc > 1:2781405/1‑71 (MQ=255) acGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACgg > 1:987542/1‑49 (MQ=255) acGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACgg > 1:1731970/1‑49 (MQ=255) acGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCgg > 1:2704954/1‑71 (MQ=255) cGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCtttt < 1:2035812/66‑1 (MQ=255) gTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTg > 1:137260/1‑71 (MQ=255) tAGGTTTTTTCCCACGGTTTCGGGCGCGGTGACTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGc > 1:4033258/1‑71 (MQ=255) ggTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACg < 1:523984/43‑1 (MQ=255) ggTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCa > 1:2794086/1‑71 (MQ=255) gTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGtt < 1:365742/39‑1 (MQ=255) gTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGc < 1:2616308/57‑1 (MQ=255) gTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCAc < 1:1207535/71‑1 (MQ=255) gTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCAc > 1:1578675/1‑71 (MQ=255) gTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCAc < 1:1676561/71‑1 (MQ=255) gTTTTTTCCCACGGTTTCGGGCGCGGTGACTGCACGGTTACGGCTTTGGCtt < 1:2686594/52‑1 (MQ=255) gTTTTTTCCCACGGTTTCGGGCGCGGTGACTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCAc < 1:1219331/71‑1 (MQ=255) tttttCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCg > 1:747411/1‑71 (MQ=255) ttttCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCgg > 1:1895443/1‑71 (MQ=255) ttttCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCg < 1:2671112/70‑1 (MQ=255) ttttCCCACGGTTTCGGGCGCGGTGACTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCgg > 1:2135010/1‑71 (MQ=255) tttCCCACGGTTTCGGGCGCGGTGTCTGCACGGTTACGGCTTTGGCTTCCTGCttttt > 1:3113953/1‑58 (MQ=255) tttCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCttttt > 1:1540899/1‑58 (MQ=255) tttCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCttt > 1:3097804/1‑56 (MQ=255) tttCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGt > 1:112160/1‑71 (MQ=255) tttCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGt > 1:2927625/1‑71 (MQ=255) ttCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGt < 1:1325056/70‑1 (MQ=255) ttCCCACGGTTTCGGGCGCGGTGACTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGtt > 1:1188241/1‑71 (MQ=255) cccACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCgg < 1:208248/58‑1 (MQ=255) cccACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGttt < 1:1813449/70‑1 (MQ=255) cccACGGTTTCGGGCGCGGTGACTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGttt < 1:3152936/70‑1 (MQ=255) ccACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTg < 1:2568901/70‑1 (MQ=255) ccACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACAGGTTTg < 1:2475442/70‑1 (MQ=255) cACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGc < 1:787154/35‑1 (MQ=255) cACGGTTTCGGGCGCGGTGACTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTg < 1:1927500/69‑1 (MQ=255) aCGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTcc > 1:3392644/1‑44 (MQ=255) aCGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCg < 1:3160667/63‑1 (MQ=255) aCGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTa < 1:1749523/71‑1 (MQ=255) ggTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCg < 1:1866618/61‑1 (MQ=255) gTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCt < 1:2623200/38‑1 (MQ=255) gTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCt < 1:3721190/38‑1 (MQ=255) ttCGGGCGCGGTGACTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTAt > 1:2145734/1‑67 (MQ=255) tCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGc > 1:2263657/1‑52 (MQ=255) cGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCtttt < 1:1533239/44‑1 (MQ=255) gggCGCGGTGACTGCACTGTTACGGCTTTGGCTTCCTGCtttt < 1:1700896/43‑1 (MQ=38) gTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGttt < 1:1505071/52‑1 (MQ=255) tGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGtt < 1:2701392/50‑1 (MQ=255) tGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAAt < 1:270557/71‑1 (MQ=255) ggCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGt > 1:2590312/1‑39 (MQ=255) gCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCg > 1:3516956/1‑36 (MQ=255) gCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCAc < 1:771769/62‑1 (MQ=255) gCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATc < 1:3496969/70‑1 (MQ=255) | TATCGCCCTTCACTTTCTCGAAAGAAGGCAGCAACACGTAGGTTTTTTCCCACGGTTTCGGGCGCGGTGGCTGCACGGTTACGGCTTTGGCTTCCTGCTTTTTCGGTGCCACCGGTTTGTTATCCGCGCACGGCAAATC > minE/2022997‑2023135 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |