| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,039,506 | C→T | 22.8% | V137I (GTT→ATT) | ribB ← | 3,4 dihydroxy‑2‑butanone‑4‑phosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,039,506 | 0 | C | T | 22.8% | 215.3 / 58.1 | 105 | V137I (GTT→ATT) | ribB | 3,4 dihydroxy‑2‑butanone‑4‑phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base C (39/42); new base T (12/12); total (51/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 108 total aligned reads displayed.
AGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACG > minE/2039436‑2039575 | aGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAAc < 1:79865/71‑1 (MQ=255) aTCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGt > 1:625921/1‑71 (MQ=255) aTCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGt > 1:3718345/1‑71 (MQ=255) aTCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGt > 1:2072300/1‑71 (MQ=255) aTCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGt > 1:2748426/1‑71 (MQ=255) tCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTg < 1:2805347/71‑1 (MQ=255) cAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTgg > 1:3968127/1‑71 (MQ=255) cAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTgg > 1:2619755/1‑71 (MQ=255) aaTAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGc > 1:3292118/1‑71 (MQ=255) gTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAg < 1:293633/70‑1 (MQ=255) gTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAg < 1:1622280/70‑1 (MQ=255) gTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAg < 1:1835470/70‑1 (MQ=255) ttGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAg < 1:1742509/69‑1 (MQ=255) gCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGAc > 1:480954/1‑70 (MQ=255) gCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGAc > 1:2560220/1‑70 (MQ=255) tCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGAtt > 1:1898433/1‑71 (MQ=255) aGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGAtt > 1:3805151/1‑69 (MQ=255) gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAg < 1:3072016/71‑1 (MQ=255) gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:2039012/71‑1 (MQ=255) gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:2043814/71‑1 (MQ=255) gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:1554169/71‑1 (MQ=255) gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:2770717/71‑1 (MQ=255) gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:2552400/71‑1 (MQ=255) gTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:3797405/71‑1 (MQ=255) tATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGa < 1:4054258/71‑1 (MQ=255) tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAg < 1:608741/58‑1 (MQ=255) tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAg < 1:3390780/58‑1 (MQ=255) tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGAtt > 1:3897542/1‑65 (MQ=255) tGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGAt < 1:1927983/70‑1 (MQ=255) aCCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGAt > 1:2126492/1‑68 (MQ=255) aCCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCt > 1:1492745/1‑70 (MQ=255) gCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGa < 1:2797566/58‑1 (MQ=255) gCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGa < 1:2652324/69‑1 (MQ=255) cACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCt > 1:125571/1‑65 (MQ=255) cACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGt > 1:3142501/1‑71 (MQ=255) cACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGt > 1:2602971/1‑71 (MQ=255) aCGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGAtt > 1:1830381/1‑57 (MQ=255) aCGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGtt > 1:184426/1‑71 (MQ=255) gcgTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGtttt > 1:2241066/1‑71 (MQ=255) cgTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTg < 1:1479117/71‑1 (MQ=255) cgTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTg < 1:1212468/71‑1 (MQ=255) gTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTgc < 1:842066/71‑1 (MQ=255) tCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGAtt > 1:1959119/1‑52 (MQ=255) tCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:1418222/55‑1 (MQ=255) aGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGa > 1:2042161/1‑48 (MQ=255) aGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTgcgc < 1:2868935/70‑1 (MQ=255) gTACACCACCTGCCTGAGCGCGAAGTGGGAAAAtgtg > 1:3210468/1‑37 (MQ=255) gTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACTATTCAGATCTGACGGTTTTGCGCCa < 1:1208254/71‑1 (MQ=255) gTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:438081/52‑1 (MQ=255) tACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCAt > 1:4068951/1‑71 (MQ=255) tACACCACCTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCAt < 1:2406587/71‑1 (MQ=255) tACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:388464/51‑1 (MQ=255) tACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCAt > 1:3532234/1‑71 (MQ=255) acacCACCTGCCTGAGCGCGAAGTGGGAAACTGTGGCCAGGACGAtt > 1:1075371/1‑47 (MQ=255) caccacCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCa > 1:15531/1‑48 (MQ=255) caccacCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGAt > 1:337537/1‑51 (MQ=255) caccacCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGAt > 1:3024467/1‑51 (MQ=255) caccacCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCg > 1:418021/1‑71 (MQ=255) caccacCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCg < 1:3841672/71‑1 (MQ=255) accaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGtt > 1:1021128/1‑59 (MQ=255) accaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGtt > 1:720258/1‑59 (MQ=255) ccaccTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGc < 1:622164/71‑1 (MQ=255) ccaccTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGc < 1:1535573/71‑1 (MQ=255) ccaccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGc < 1:133330/71‑1 (MQ=255) caccTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCa < 1:1116565/71‑1 (MQ=255) caccTGCCTGAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCa < 1:1049869/71‑1 (MQ=255) caccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCa < 1:2966853/71‑1 (MQ=255) caccTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCa < 1:2464092/71‑1 (MQ=255) caccTGCCTGAGCGCGAAGTGGGAAAACGTGGCAAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGc > 1:1362549/1‑70 (MQ=255) cctgcctgAGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCaa > 1:3125209/1‑70 (MQ=255) cctgcctgAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAAt < 1:71811/71‑1 (MQ=255) ctgcctgAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:2679659/43‑1 (MQ=255) ctgcctgAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAg < 1:3605285/43‑1 (MQ=255) tgcctgAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGt < 1:273196/52‑1 (MQ=255) ctgAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTg > 1:923653/1‑71 (MQ=255) tgAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTgc > 1:3918864/1‑71 (MQ=255) aGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAg < 1:1733204/36‑1 (MQ=255) aGCGCGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTg > 1:3760150/1‑50 (MQ=255) aGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTgcgc > 1:361813/1‑71 (MQ=255) aGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTgcgc > 1:2926852/1‑71 (MQ=255) aGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTgcgc > 1:1352132/1‑71 (MQ=255) gcgcGAAGTGGGAAAATGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTgcgc > 1:648154/1‑70 (MQ=255) gcgcGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGcgcg < 1:1549065/71‑1 (MQ=255) acgcgAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGcgcg < 1:1676375/70‑1 (MQ=255) cgcgAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGa < 1:2631663/71‑1 (MQ=255) gcgAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATc > 1:869335/1‑54 (MQ=255) aaGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGtt > 1:340243/1‑41 (MQ=255) aaGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACgg < 1:2418937/71‑1 (MQ=255) aaGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACgg > 1:2381563/1‑71 (MQ=255) ggAAAACGTGGCCAGGACGATTCAGATCTGACGGtttt < 1:25349/38‑1 (MQ=255) ggAAAACGTGGCCAGGACGATTCAGATCTGACGGttt > 1:2265976/1‑37 (MQ=255) ggAAAACGTGGCCAGGACGATTCAGATCTGACGGttt > 1:166407/1‑37 (MQ=255) ggAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGt < 1:535867/70‑1 (MQ=255) ggAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGt < 1:1772767/70‑1 (MQ=255) ggAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGt < 1:436444/70‑1 (MQ=255) gAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTaa < 1:2514107/71‑1 (MQ=255) aaaaCGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCaa > 1:302521/1‑49 (MQ=255) aaaaCGTGG‑CAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCg > 1:3246502/1‑51 (MQ=255) aCGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACg < 1:3049631/71‑1 (MQ=255) cGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACg > 1:1750062/1‑70 (MQ=255) | AGATCAATAGTTGCTTCAGTATGACCGCCACGCGTCAGTACACCACCTGCCTGAGCGCGAAGTGGGAAAACGTGGCCAGGACGATTCAGATCTGACGGTTTTGCGCCATCGGCAATCGCTGCGCGAACGGTCGTAATACG > minE/2039436‑2039575 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |