| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,040,713 | C→T | 22.9% | D40D (GAC→GAT) | yqiH → | predicted periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,040,713 | 0 | C | T | 22.9% | 196.6 / 54.5 | 96 | D40D (GAC→GAT) | yqiH | predicted periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base C (46/28); new base T (10/12); total (56/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.05e-01 | |||||||||||
CTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAA > minE/2040644‑2040782 | cTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACa < 1:215809/71‑1 (MQ=255) gTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACa > 1:3147952/1‑69 (MQ=255) tGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGc > 1:3882292/1‑71 (MQ=255) agcagcATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCg < 1:395328/70‑1 (MQ=255) agcagcATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGa < 1:2421381/71‑1 (MQ=255) gcagcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCg > 1:2742142/1‑69 (MQ=255) gcagcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCg > 1:2163288/1‑69 (MQ=255) cagcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGaa < 1:537543/70‑1 (MQ=255) caTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCAt > 1:2133059/1‑71 (MQ=255) aTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCAtt < 1:3939197/71‑1 (MQ=255) aTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCAtt < 1:1450899/71‑1 (MQ=255) aTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCAt > 1:3476852/1‑70 (MQ=255) ttGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCAtt > 1:706682/1‑70 (MQ=255) ttGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTa < 1:342467/71‑1 (MQ=255) gCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTaa > 1:2626648/1‑70 (MQ=255) gCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTaa > 1:1693825/1‑70 (MQ=255) gCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTaa > 1:4013588/1‑70 (MQ=255) ccTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTaaa < 1:3488610/70‑1 (MQ=255) ccTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTaaa < 1:2340398/70‑1 (MQ=255) ccTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTaaa < 1:289464/70‑1 (MQ=255) ggTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTAc > 1:1597760/1‑71 (MQ=255) gCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGAc > 1:2984575/1‑45 (MQ=255) gCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGAc > 1:1246020/1‑45 (MQ=255) ccgtcatcCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAAt > 1:1781540/4‑71 (MQ=255) ccATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCAtt < 1:2290025/58‑1 (MQ=255) ccATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCAtt < 1:165729/58‑1 (MQ=255) ccATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAg < 1:197488/54‑1 (MQ=255) ccATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAg < 1:2531344/54‑1 (MQ=255) ccATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAg < 1:1456430/54‑1 (MQ=255) ccATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAAt > 1:443107/1‑71 (MQ=255) atcatcCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGa < 1:225130/50‑1 (MQ=255) atcatcCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTaaa < 1:3578442/59‑1 (MQ=255) atcatcCAGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATc > 1:1577274/1‑70 (MQ=255) tcatcCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTaa < 1:4138628/57‑1 (MQ=255) tcatcCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACt < 1:1529118/61‑1 (MQ=255) tcataCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCa > 1:3253758/1‑70 (MQ=255) atcCCTGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAg > 1:1318620/1‑71 (MQ=255) atcCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAgg > 1:2490831/1‑44 (MQ=255) atcCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCa > 1:2866402/1‑51 (MQ=255) atcCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCa > 1:3108477/1‑68 (MQ=255) atcCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAg > 1:3412216/1‑71 (MQ=255) tcCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAg > 1:2944277/1‑70 (MQ=255) tcCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAg > 1:1874150/1‑70 (MQ=255) cccGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAg > 1:1576177/1‑69 (MQ=255) ccGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTaaaa > 1:3975347/1‑54 (MQ=255) ccGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTa > 1:2415945/1‑58 (MQ=255) ccGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTa > 1:169045/1‑58 (MQ=255) ccGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTa > 1:1182771/1‑70 (MQ=255) cGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaa < 1:3815019/71‑1 (MQ=255) cGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaa < 1:3332207/71‑1 (MQ=255) ggATCGTACCCGTATCATAATGAATGAGTCAGACaaa > 1:1642820/1‑37 (MQ=255) ggATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAAc > 1:1916109/1‑53 (MQ=255) ggATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaa < 1:3813970/71‑1 (MQ=255) ggATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaa > 1:1284225/1‑70 (MQ=255) aTCGTACCCGTATCATAATGAATGAGTAAGACAAAGGCGAAGCAtt < 1:3615155/46‑1 (MQ=255) tCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACt < 1:868927/51‑1 (MQ=255) cccGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGccc > 1:620191/1‑71 (MQ=255) ccGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCt < 1:592124/71‑1 (MQ=255) ccGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCt < 1:273616/71‑1 (MQ=255) gTATCATAATGAATGAGTCAGATAAAGGCGAAGCa > 1:3342288/1‑35 (MQ=255) tATCATAATGAATGAGTCAGATAAAGGCGAAGCATTaa > 1:2627695/1‑38 (MQ=255) tATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATc < 1:497738/71‑1 (MQ=255) tATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTa < 1:2439727/44‑1 (MQ=255) aTCATAATGAATGAGTCAGACAAAGGCGAAGCAtt < 1:1589965/35‑1 (MQ=255) tCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaa < 1:78143/57‑1 (MQ=255) tCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaa < 1:3810541/57‑1 (MQ=255) tCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaaa > 1:633222/1‑58 (MQ=255) tCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCt < 1:403625/70‑1 (MQ=255) tCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTg > 1:72166/1‑71 (MQ=255) aTAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACt > 1:4069185/1‑38 (MQ=255) aTAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTa > 1:957768/1‑40 (MQ=255) aTAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTa > 1:1465361/1‑40 (MQ=255) aTAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTa > 1:211229/1‑40 (MQ=255) aTAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCt > 1:2086476/1‑59 (MQ=255) aTAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTgg < 1:51381/70‑1 (MQ=255) aTAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGc > 1:872013/1‑71 (MQ=255) aTAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGc > 1:3070476/1‑71 (MQ=255) tAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCaaa < 1:217407/48‑1 (MQ=255) aatgaatgAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTAta > 1:1664009/1‑64 (MQ=255) aatgaatgAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGcc > 1:117439/1‑70 (MQ=255) atgaatgaGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaa > 1:3075505/1‑51 (MQ=255) tgaatgaGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaaa > 1:1709966/1‑52 (MQ=255) atgaGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTTCCCTATCTGGCCCaaa < 1:1993361/69‑1 (MQ=255) atgaGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACa > 1:1830585/1‑71 (MQ=255) aGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACa > 1:588728/1‑68 (MQ=255) aGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaa < 1:765742/45‑1 (MQ=255) aGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATg < 1:417691/70‑1 (MQ=255) tCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATgg < 1:2825369/69‑1 (MQ=255) aGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaa < 1:3247396/40‑1 (MQ=255) aGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGa > 1:2728269/1‑68 (MQ=255) aGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGa > 1:3213894/1‑68 (MQ=255) aGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGa > 1:3318513/1‑68 (MQ=255) aCAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGGTagaa < 1:2660476/71‑1 (MQ=255) aCAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATag > 1:553789/1‑69 (MQ=255) aCAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATag > 1:2232416/1‑69 (MQ=255) aCAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATag > 1:3914240/1‑69 (MQ=255) aCAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATag > 1:1355005/1‑69 (MQ=255) aCAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATag > 1:882165/1‑69 (MQ=255) cAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAAc > 1:2729769/1‑60 (MQ=255) cAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATagaa < 1:1130940/70‑1 (MQ=255) | CTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGAA > minE/2040644‑2040782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |