| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,041,438 | G→A | 43.1% | D32N (GAT→AAT) | yqiI → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,041,438 | 0 | G | A | 43.1% | 35.1 / 89.5 | 72 | D32N (GAT→AAT) | yqiI | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (23/18); new base A (11/20); total (34/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.94e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
TTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTG > minE/2041373‑2041508 | tttttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATAcc > 1:137328/1‑71 (MQ=255) tGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGt < 1:3158838/70‑1 (MQ=255) tGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGt < 1:1144673/70‑1 (MQ=255) ttAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGgaga > 1:3890484/1‑55 (MQ=255) ttAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGt > 1:64478/1‑71 (MQ=255) ttAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGt > 1:321383/1‑71 (MQ=255) ttAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGt > 1:1070941/1‑71 (MQ=255) tAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGt < 1:999468/70‑1 (MQ=255) tAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGt < 1:1096836/70‑1 (MQ=255) tAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGt < 1:784747/70‑1 (MQ=255) ttCATTACCTGCATGGGCAATGGACTTCTATGCTGAACATGAAGGTGGAAATACCGTTGTc < 1:1370773/61‑1 (MQ=255) ttCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTc < 1:428959/61‑1 (MQ=255) ttCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCAt < 1:3254247/63‑1 (MQ=255) ttCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACg < 1:1924729/71‑1 (MQ=255) cATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCAt > 1:3312573/1‑61 (MQ=255) cATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTa > 1:591395/1‑71 (MQ=255) ttACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGAt < 1:1892457/48‑1 (MQ=255) aCCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACg > 1:1587069/1‑65 (MQ=255) aCCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTAcc > 1:722518/1‑69 (MQ=255) ccTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAg < 1:3352134/71‑1 (MQ=255) gCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAAt > 1:2204240/1‑71 (MQ=255) aTGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAAtct < 1:4036717/71‑1 (MQ=255) tGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACg < 1:1516171/58‑1 (MQ=255) tGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACg < 1:3251476/58‑1 (MQ=255) tGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACg < 1:2710836/58‑1 (MQ=255) ggCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTa < 1:1888101/71‑1 (MQ=255) gCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATctct < 1:1511447/69‑1 (MQ=255) gCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTAt < 1:1499910/71‑1 (MQ=255) cAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGt < 1:1659058/50‑1 (MQ=255) aTGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATccc > 1:3549086/1‑71 (MQ=255) tGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATccc > 1:700241/1‑70 (MQ=255) tGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATccc > 1:1248581/1‑70 (MQ=255) ggACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTa > 1:3444893/1‑48 (MQ=255) ggACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATccc > 1:238632/1‑69 (MQ=255) gACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAg > 1:2072837/1‑71 (MQ=255) cTGCTATGCTGAACATGAAGGTGGAGATACCGTTGt < 1:2357653/36‑1 (MQ=255) cTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCg < 1:1751174/71‑1 (MQ=255) cTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCg < 1:509631/71‑1 (MQ=255) cTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCg < 1:1758890/71‑1 (MQ=255) tGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTgg < 1:1507259/41‑1 (MQ=255) tGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTgg < 1:3387083/41‑1 (MQ=255) cTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATg > 1:2750324/1‑71 (MQ=255) cTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATg > 1:834105/1‑71 (MQ=255) cTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAAtct > 1:2247830/1‑56 (MQ=255) tATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGt < 1:128277/39‑1 (MQ=255) tATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATgg > 1:3044125/1‑71 (MQ=255) tATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATgg > 1:3450778/1‑71 (MQ=255) tATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTa < 1:1781259/41‑1 (MQ=255) gCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGa < 1:3370838/48‑1 (MQ=255) cTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTAc < 1:3321470/38‑1 (MQ=255) cTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTAc < 1:1599623/38‑1 (MQ=255) cTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACg > 1:208792/1‑39 (MQ=255) cTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTa > 1:1868565/1‑41 (MQ=255) cTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGaa > 1:638641/1‑48 (MQ=255) cTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGaa > 1:2215375/1‑48 (MQ=255) gAACATGTAGGTGGAAATACCGTTGTCATTGGTTACGTa > 1:1559393/1‑39 (MQ=37) gAACATGAAGGTGGAGATACCGTTGGCATTGGTTACGTACCaa < 1:630302/43‑1 (MQ=255) gAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGt > 1:2408200/1‑38 (MQ=255) gAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTa > 1:3669564/1‑39 (MQ=255) gAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTa > 1:3611642/1‑39 (MQ=255) aaCATGAAGGTGTAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTaaaa < 1:205329/69‑1 (MQ=255) aaCATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTaaaa < 1:3467827/69‑1 (MQ=255) aaCATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTaaaa > 1:2291119/1‑69 (MQ=255) aaCATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTaaaat < 1:702079/70‑2 (MQ=255) aaCATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTaaaaa < 1:2301868/70‑1 (MQ=255) cATGAAGGTGGAGATACCGTTGTCATTGGTTACGTa > 1:3341063/1‑36 (MQ=255) cATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGc < 1:1262047/57‑1 (MQ=255) aTGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGt < 1:1832918/71‑1 (MQ=255) tGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAAt > 1:1721902/1‑42 (MQ=255) tGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAAt > 1:911093/1‑42 (MQ=255) tGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAg > 1:1094359/1‑54 (MQ=255) tGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGt < 1:879512/70‑1 (MQ=255) ggAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAAt > 1:2444560/1‑71 (MQ=255) gagaTACCGTTGTCATTGGTTACGTACCAATAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATc < 1:4026298/71‑1 (MQ=255) gaTACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTg < 1:2105309/71‑1 (MQ=255) | TTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCTG > minE/2041373‑2041508 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |