| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,042,482 | C→T | 15.8% | M57I (ATG→ATA) | glgS ← | predicted glycogen synthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,042,482 | 0 | C | T | 15.8% | 291.6 / 40.9 | 114 | M57I (ATG→ATA) | glgS | predicted glycogen synthesis protein |
| Reads supporting (aligned to +/- strand): ref base C (51/44); new base T (4/14); total (55/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.97e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
TAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGTTAC > minE/2042414‑2042549 | tAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGcat > 1:1752539/1‑71 (MQ=255) aTACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGtatca > 1:816104/1‑71 (MQ=255) cAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCt > 1:3317205/1‑71 (MQ=255) aaCACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCt > 1:1841177/1‑69 (MQ=255) acacTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGtt < 1:1610759/71‑1 (MQ=255) cacTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTg > 1:3201975/1‑68 (MQ=255) cTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGAc < 1:2267727/71‑1 (MQ=255) aaaCGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACaa < 1:3465688/70‑1 (MQ=255) aaaCGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACaa < 1:2230153/70‑1 (MQ=255) ccATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAAt < 1:2877619/64‑1 (MQ=255) aTCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTg < 1:1807779/54‑1 (MQ=255) tCAGGCCCGTTATATCAGTTCTCTAACTCCAGCTCTCTTGCCTGcatc < 1:3634078/48‑1 (MQ=255) cAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGAc < 1:3185128/57‑1 (MQ=255) cAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATaa > 1:3049781/1‑71 (MQ=255) aGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACaa > 1:3739365/1‑58 (MQ=255) aGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAAt < 1:1038219/59‑1 (MQ=255) aGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAAt < 1:819646/59‑1 (MQ=255) aGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCt > 1:3519433/1‑50 (MQ=255) aGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAAc > 1:3438963/1‑71 (MQ=255) aGGCCCGTTATATCAGTGCTCTAACTACAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAAc > 1:2603747/1‑71 (MQ=255) cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGAc < 1:1705303/53‑1 (MQ=255) cccGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTg < 1:1308834/71‑1 (MQ=255) cGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGcatcat > 1:2383382/1‑43 (MQ=255) gTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGAc < 1:2637337/50‑1 (MQ=255) gTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTgcg > 1:1147873/1‑70 (MQ=255) gTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTgcg > 1:3710426/1‑70 (MQ=255) gTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTgcg > 1:404965/1‑70 (MQ=255) ttATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTg < 1:3153653/47‑1 (MQ=255) ttATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACaa < 1:59436/51‑1 (MQ=255) ttATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTgcgc > 1:358091/1‑70 (MQ=255) atatCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAAt > 1:3734700/1‑50 (MQ=255) atatCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCaa < 1:347157/70‑1 (MQ=255) atatCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCaa < 1:1347729/70‑1 (MQ=255) atatCAGTGCTCTAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCaa < 1:3363248/70‑1 (MQ=255) atatCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCaa < 1:1489803/70‑1 (MQ=255) atatCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCaa > 1:3138263/1‑70 (MQ=255) atatCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCaa < 1:1559952/70‑1 (MQ=255) atatCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCaa > 1:1722765/1‑70 (MQ=255) atatCAGTGCTCTAACT‑CAGCTCTCTTGCCTGTATCATCTGTTGACAAt > 1:2721989/1‑49 (MQ=255) tatCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGAc < 1:3686502/46‑1 (MQ=255) tatCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGAc < 1:2071720/46‑1 (MQ=255) tatCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCaaaa > 1:863682/1‑71 (MQ=255) tatCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCaaaa > 1:88305/1‑71 (MQ=255) atCAGTGCTCTAACTCCAGCTCTCTTGCCTGAATCATCTGTTGACAATACCAGGCATaa < 1:211443/59‑1 (MQ=255) tCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGtt < 1:3618504/41‑1 (MQ=255) tCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGAc < 1:405854/44‑1 (MQ=255) tCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGAc < 1:2318086/44‑1 (MQ=255) tCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAAc > 1:1035779/1‑70 (MQ=255) tCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAAc < 1:4025800/70‑1 (MQ=255) cAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGa > 1:2108764/1‑42 (MQ=255) tGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGt > 1:3818524/1‑71 (MQ=255) ctctAACTCCAGCTCTCTTGCCTGTATCATCTGTTGAc < 1:823698/38‑1 (MQ=255) ctctAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATa > 1:3035914/1‑42 (MQ=255) ctctAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAg < 1:2292030/46‑1 (MQ=255) ctctAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTc < 1:51704/71‑1 (MQ=255) tAACTCCAGCTCTCTTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCt < 1:2174524/69‑1 (MQ=255) tAACTCCAGCTCTCTTGCCTGCATCATCTGTTGAc < 1:580360/35‑1 (MQ=255) tAACTCCAGCTCTCTTGCCTGCATCATCTGTTGAc < 1:2443687/35‑1 (MQ=255) tAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCTCAAAACCAGGTTCTAt > 1:171762/1‑71 (MQ=255) tAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCt < 1:2744008/69‑1 (MQ=255) tAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTAt > 1:2241366/1‑71 (MQ=255) aaCTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATg > 1:3311604/1‑71 (MQ=255) aCTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTTCGCAAAACCAGGTTCTATGt > 1:2005613/1‑71 (MQ=255) aCTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGt > 1:1691112/1‑71 (MQ=255) aCTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGt > 1:260657/1‑71 (MQ=255) aCTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGt > 1:1575802/1‑71 (MQ=255) aCTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGt > 1:3272646/1‑71 (MQ=255) ccAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCa > 1:1393280/1‑58 (MQ=255) ccAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGttct > 1:4102735/1‑71 (MQ=255) cAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATaa > 1:2611918/1‑43 (MQ=255) cAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGttc < 1:1562404/69‑1 (MQ=255) ctctctTGCCTGCATCATCTGTTGACAATACCAGGCATaa < 1:250757/40‑1 (MQ=255) ctctctTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTc < 1:1528218/59‑1 (MQ=255) ctctctTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCAt > 1:3270338/1‑71 (MQ=255) ctctctTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCAt > 1:1930686/1‑71 (MQ=255) tctctTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCaaaa > 1:2879584/1‑50 (MQ=255) ctctTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATc > 1:2812147/1‑70 (MQ=255) ctctTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATc > 1:1215859/1‑70 (MQ=255) tctTGCCTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATc < 1:437665/69‑1 (MQ=255) tctTGCCTGCATCATCTGTTGACAATACCAGGCAt < 1:2838244/35‑1 (MQ=255) ttGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCaaaa > 1:2973904/1‑46 (MQ=255) ttGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGttct > 1:3936636/1‑62 (MQ=255) ttGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCa > 1:499910/1‑65 (MQ=255) ttGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATg > 1:1302707/1‑71 (MQ=255) ttGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTAATCCATg > 1:2915506/1‑71 (MQ=255) ttGCCTGCATCATCTGTTGA‑AATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCa > 1:2662049/1‑64 (MQ=255) tGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGt > 1:2465733/1‑71 (MQ=255) gCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGt > 1:267887/1‑70 (MQ=255) gCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGt > 1:1373500/1‑70 (MQ=255) ccTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCaaa < 1:3209863/42‑1 (MQ=255) ccTGTATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCAt < 1:1790456/67‑1 (MQ=255) ccTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGTTa < 1:3082801/71‑1 (MQ=255) cTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGTTAc < 1:3002634/71‑1 (MQ=255) cTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGTTAc < 1:1124749/71‑1 (MQ=255) cTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGTTAc < 1:2639641/71‑1 (MQ=255) tGCATCATCTGTTGACAATACCAGGCATAACGTGCGCaaa < 1:515530/40‑1 (MQ=255) tGCATCATCTGTTGACAATACCAGGCATAACGTGCGCaaa < 1:3974694/40‑1 (MQ=255) tGCATCATCTGTTGACAATACCAGGCATAACGTGCGCaaa < 1:3511619/40‑1 (MQ=255) gCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGTTAc > 1:3085331/1‑69 (MQ=255) catcatCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGt < 1:249015/65‑1 (MQ=255) | TAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAAACCAGGTTCTATGTTCTTCATCCATGTTAC > minE/2042414‑2042549 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |