| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,066,377 | G→A | 14.3% | noncoding (67/76 nt) | ileX → | tRNA‑Ile |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,066,377 | 0 | G | A | 14.3% | 110.5 / 9.6 | 42 | noncoding (67/76 nt) | ileX | tRNA‑Ile |
| Reads supporting (aligned to +/- strand): ref base G (12/24); new base A (3/3); total (15/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTT > minE/2066307‑2066446 | ccATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCcagcag < 1:790497/71‑1 (MQ=255)ccATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCcagcag > 1:3847552/1‑71 (MQ=255) aggCCCTTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAaggg < 1:1904831/70‑1 (MQ=255) gCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCcagcag > 1:1885658/1‑66 (MQ=255) cccTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGccac < 1:1674132/71‑1 (MQ=255) tttAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAAGGGccacc > 1:2112059/2‑70 (MQ=255) cTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGccacca < 1:956522/71‑1 (MQ=255) ttAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAggg < 1:67619/63‑1 (MQ=255) ttAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAAGGGccacca > 1:2498572/1‑70 (MQ=255) tAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAgg > 1:3434697/1‑61 (MQ=255) tAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGccac > 1:2721694/1‑67 (MQ=255) aGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAg < 1:1772515/69‑1 (MQ=255) aGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAg < 1:4120451/69‑1 (MQ=255) aGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAg < 1:3260932/69‑1 (MQ=255) aGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAg < 1:3544315/69‑1 (MQ=255) aGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGa < 1:2062071/70‑1 (MQ=255) cTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCcagcag > 1:586414/1‑57 (MQ=255) aGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAAGGGccaccaaa > 1:2625063/1‑63 (MQ=255) aGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAAGGGCCACCAAatcat < 1:2154691/69‑4 (MQ=255) aGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAAGGGCCACCAAatcat < 1:2449297/69‑4 (MQ=255) tGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGa < 1:59521/63‑1 (MQ=255) gTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCcagcag > 1:1239018/1‑50 (MQ=255) gTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAgg > 1:1850312/1‑51 (MQ=255) gTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGa < 1:2744322/61‑1 (MQ=255) ttAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCcagcag < 1:1437258/49‑1 (MQ=255) ttAGAGCAGGCGACTCATAATCGCTTGCTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGa < 1:223860/60‑1 (MQ=255) tAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGccac > 1:2936499/1‑55 (MQ=255) tAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGc > 1:2826610/1‑71 (MQ=255) agCAGGCGACTCATAATCGCTTGGGCGCTGGTTCAAGTCCAGCAGGGGCCACCAGa < 1:3356188/56‑1 (MQ=255) ggCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCa < 1:2603009/59‑1 (MQ=255) cGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGaa < 1:885083/71‑1 (MQ=255) gACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGaaa > 1:4129122/1‑71 (MQ=255) gACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGaaa > 1:3758909/1‑71 (MQ=255) gACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGaaa < 1:3715632/71‑1 (MQ=255) gACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGaaa > 1:418507/1‑71 (MQ=255) cTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATc < 1:2256609/71‑1 (MQ=255) tCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGccacca < 1:1978814/44‑1 (MQ=255) tCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGa < 1:214694/46‑1 (MQ=255) tCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAAGGGCCACCAAatc < 1:2157524/48‑2 (MQ=38) aTAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGt > 1:1697422/1‑70 (MQ=255) tAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCa < 1:3486864/50‑1 (MQ=255) aTCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGaa > 1:350823/1‑62 (MQ=255) aTCGCTTGGGCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGc < 1:3808411/71‑1 (MQ=255) gCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctt > 1:3736302/1‑71 (MQ=255) tGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCcttttt > 1:3136172/1‑71 (MQ=255) tCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCg < 1:2520910/57‑1 (MQ=255) cTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttcttttt < 1:4048854/71‑1 (MQ=255) ggTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTta > 1:2511947/1‑70 (MQ=255) gTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTta < 1:1691565/69‑1 (MQ=255) gTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTta < 1:722160/69‑1 (MQ=255) ttCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGt < 1:2363819/51‑1 (MQ=255) cAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTtatata > 1:2964346/1‑70 (MQ=255) cagcagGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTta > 1:629353/1‑60 (MQ=255) gcagGGGCCACCAGATATAGCAAAGGCTGACGAGaa < 1:3347453/36‑1 (MQ=255) gcagGGGCCACCAGATATAGCAAAGGCTGACGAGaa < 1:2825457/36‑1 (MQ=255) gcagGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCct < 1:680299/47‑1 (MQ=255) cagGGGCCACCAGATATAGCAAAGGCTGACGAGaa < 1:4077706/35‑1 (MQ=255) agGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACttt > 1:1208320/1‑71 (MQ=255) | CCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTT > minE/2066307‑2066446 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |