| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,072,048 | A→T | 15.9% | intergenic (‑88/‑131) | ygjH ← / → ebgR | conserved hypothetical protein/DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,072,048 | 0 | A | T | 15.9% | 186.9 / 25.7 | 75 | intergenic (‑88/‑131) | ygjH/ebgR | conserved hypothetical protein/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (31/32); new base T (7/5); total (38/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAGGT > minE/2071978‑2072117 | tAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGa > 1:2473560/1‑71 (MQ=255) aaaaTTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGAt > 1:2909390/1‑71 (MQ=255) aaaTTTTACTAAAGTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGAt > 1:3327308/1‑70 (MQ=255) aaaTTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGAt > 1:588453/1‑70 (MQ=255) aaaTTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGAt > 1:2612748/1‑70 (MQ=255) aaaTTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGAt > 1:4073224/1‑70 (MQ=255) aaaTTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGAt > 1:134517/1‑70 (MQ=255) aaaTTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATc < 1:3975109/71‑1 (MQ=255) tttACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACAt > 1:3016862/1‑71 (MQ=255) tACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACAtt < 1:3486635/70‑1 (MQ=255) cTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGt < 1:501291/71‑1 (MQ=255) cTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGt < 1:3588682/71‑1 (MQ=255) aaCTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAg > 1:3332902/1‑71 (MQ=255) aaCTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAg > 1:1127344/1‑71 (MQ=255) aaCTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAg > 1:2716116/1‑71 (MQ=255) aaCTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAg > 1:3794556/1‑71 (MQ=255) aCTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGAt > 1:3943840/1‑58 (MQ=255) cTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGcc > 1:2501550/1‑71 (MQ=255) taGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCt < 1:2419774/71‑1 (MQ=255) taGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCt > 1:174119/1‑71 (MQ=255) aGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGAt < 1:3365210/53‑1 (MQ=255) gCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCtgt < 1:4126042/71‑1 (MQ=255) gCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACAt < 1:1593311/57‑1 (MQ=255) cAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATc < 1:3692542/52‑1 (MQ=255) cAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATc < 1:446386/52‑1 (MQ=255) aaaGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATcac > 1:2689647/1‑52 (MQ=255) aaaGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATcac > 1:2876583/1‑52 (MQ=255) aaaGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATcac > 1:1267557/1‑52 (MQ=255) aaGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCtg < 1:3688487/66‑1 (MQ=255) aGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTAc > 1:308740/1‑71 (MQ=255) aGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGc < 1:1308207/63‑1 (MQ=255) aGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTAc < 1:1385280/71‑1 (MQ=255) aGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTAc > 1:952593/1‑71 (MQ=255) gTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTg > 1:1025259/1‑56 (MQ=255) gTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTAcc < 1:593190/71‑1 (MQ=255) cAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGc > 1:937328/1‑71 (MQ=255) tCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATca < 1:3630228/37‑1 (MQ=255) tCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCg < 1:3157155/43‑1 (MQ=255) tCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCg < 1:837431/43‑1 (MQ=255) atGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCa > 1:3514114/2‑70 (MQ=255) tAGGCTAAAAATGGAGAATGCAGGCGTGATCACAt < 1:3129576/35‑1 (MQ=255) tAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGa < 1:2839159/71‑1 (MQ=255) aGGCTAAAAATGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCtgt > 1:2844746/1‑48 (MQ=255) ggCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGaaa > 1:641826/1‑71 (MQ=255) ggCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGaa < 1:2896277/70‑1 (MQ=255) tAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGaa > 1:3627955/1‑67 (MQ=255) aaaaTGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACgcgc < 1:411437/71‑1 (MQ=255) aaaTGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGtt < 1:1695928/44‑1 (MQ=255) aaTGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCtt < 1:402377/71‑1 (MQ=255) aaTGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCtt < 1:651200/71‑1 (MQ=255) tGGAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCttta > 1:2601346/1‑71 (MQ=255) tGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCttta > 1:723056/1‑71 (MQ=255) tGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCttta > 1:674126/1‑71 (MQ=255) tGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCttta < 1:1308135/71‑1 (MQ=255) ggAGAATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTAc > 1:331566/1‑42 (MQ=255) gagaATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGaaa > 1:14286/1‑60 (MQ=255) agaATGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCtttattt < 1:3136967/71‑1 (MQ=255) aTGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACt > 1:279844/1‑71 (MQ=255) aTGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGCTACAGCGTCAAAGAAACGCGCTTTATTTACt > 1:2577925/1‑71 (MQ=255) tGCAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACt > 1:2597699/1‑70 (MQ=255) tGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGa < 1:3454742/53‑1 (MQ=255) tGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACt > 1:2042714/1‑70 (MQ=255) gCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTAc > 1:2384943/1‑68 (MQ=255) catgCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGaa < 1:1022873/68‑1 (MQ=255) cAGGCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGaa < 1:794984/71‑1 (MQ=255) cAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGaa < 1:3909210/71‑1 (MQ=255) cAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGa < 1:3883158/70‑1 (MQ=255) cAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGa < 1:655804/70‑1 (MQ=255) aGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGaaa < 1:2327213/71‑1 (MQ=255) gCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGaaa > 1:1375469/1‑50 (MQ=255) gCGTGTTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGaaa > 1:3713981/1‑50 (MQ=255) cGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCttta < 1:1607485/58‑1 (MQ=255) cGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGaaaa > 1:1535650/1‑69 (MQ=255) tGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAg < 1:729342/44‑1 (MQ=255) tGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCttta < 1:824979/56‑1 (MQ=255) tGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAg < 1:3059523/70‑1 (MQ=255) aTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGaaaa > 1:1474843/1‑65 (MQ=255) aTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAg > 1:2132493/1‑68 (MQ=255) aTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAg > 1:2858049/1‑68 (MQ=255) aTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAGGt > 1:1982877/1‑70 (MQ=255) aTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAGGt > 1:900696/1‑70 (MQ=255) aTCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAGGt > 1:390915/1‑70 (MQ=255) | TAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCAATCCTGTAGGCTAAAAATGGAGAATGCAGGCGTGATCACATTCGTGAGCCGCTGTGTTACCGTTACAGCGTCAAAGAAACGCGCTTTATTTACTGAAAACAGGT > minE/2071978‑2072117 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |