| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,087,666 | C→T | 13.7% | T54I (ACC→ATC) | yhaH → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,087,666 | 0 | C | T | 13.7% | 199.6 / 17.6 | 73 | T54I (ACC→ATC) | yhaH | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (36/27); new base T (3/7); total (39/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGA > minE/2087599‑2087735 | atcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCcccc > 1:214740/1‑70 (MQ=255)atcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCt < 1:698628/71‑1 (MQ=255)atcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCt < 1:1013427/71‑1 (MQ=255)atcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCt > 1:3628449/1‑71 (MQ=255)atcTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCt > 1:1671193/1‑71 (MQ=255) cTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGG‑AACGCGCTGGTGGCGAAGGCACCCTga > 1:3685066/1‑70 (MQ=255) tttACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTga > 1:890193/1‑70 (MQ=255) tttACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTga > 1:3463418/1‑70 (MQ=255) tACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTgacga > 1:290330/1‑71 (MQ=255) tACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTgacga > 1:1219648/1‑71 (MQ=255) cGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTgacgac > 1:2053620/1‑70 (MQ=255) cGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACa < 1:1255644/71‑1 (MQ=255) cGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACa < 1:1489888/71‑1 (MQ=255) ttCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCt < 1:3846677/62‑1 (MQ=255) cGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATc > 1:627650/1‑70 (MQ=255) cGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATc < 1:1072567/70‑1 (MQ=255) tGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTa < 1:3279519/70‑1 (MQ=255) tGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTAc < 1:1435427/71‑1 (MQ=255) cTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAAt > 1:3514761/1‑65 (MQ=255) cTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACgg > 1:2388767/1‑71 (MQ=255) gctgctGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTc < 1:4122076/71‑1 (MQ=255) gctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTa > 1:2111408/1‑60 (MQ=255) ctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTg < 1:690996/69‑1 (MQ=255) ctgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGgt > 1:1846493/1‑71 (MQ=255) tgGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACgg < 1:2603493/61‑1 (MQ=255) gATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGtt > 1:2126449/1‑71 (MQ=255) gATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGtt > 1:3500616/1‑71 (MQ=255) tAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGtttt < 1:254756/71‑1 (MQ=255) tAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGtttt > 1:3398503/1‑71 (MQ=255) aaaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGtttt > 1:1539748/1‑70 (MQ=255) aaaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATc < 1:87740/50‑1 (MQ=255) aaTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTAc > 1:1050062/1‑71 (MQ=255) aTGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGtttt > 1:361758/1‑67 (MQ=255) tGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGt > 1:2393196/1‑53 (MQ=255) tGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTAcc < 1:3715990/70‑1 (MQ=255) ttAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgt < 1:1853150/70‑1 (MQ=255) ttAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgt < 1:4100157/70‑1 (MQ=255) ttAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgt < 1:2751552/70‑1 (MQ=255) ttAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgtg < 1:957890/71‑1 (MQ=255) ttAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgt < 1:876128/70‑1 (MQ=255) cTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGtggtgg > 1:623841/1‑70 (MQ=255) tGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCt < 1:3714690/40‑1 (MQ=255) ggCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCg > 1:2214177/1‑71 (MQ=255) gCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgtg < 1:3314207/63‑1 (MQ=255) cAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTa < 1:2291832/57‑1 (MQ=255) cAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGt > 1:3235876/1‑71 (MQ=255) cAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGt < 1:2490690/71‑1 (MQ=255) aaCGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGt > 1:2722095/1‑70 (MQ=255) aCGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTc < 1:1243339/71‑1 (MQ=255) aCGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTc < 1:3649812/71‑1 (MQ=255) cgcgCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGgtg > 1:3390722/1‑48 (MQ=255) cgcgCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgtgg > 1:2377441/1‑60 (MQ=255) cgcgCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCa < 1:1002262/71‑1 (MQ=255) cgcgCTGGTGGCGAAGGCACCCTGACGACAATCTACGCTATTCTGGTGTTTTTACCGTGGTGGGCGGtt > 1:1080410/1‑69 (MQ=255) gcgcTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAg > 1:4118305/1‑71 (MQ=255) cgcTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGt > 1:2379924/1‑71 (MQ=255) cgcTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGt > 1:2603200/1‑71 (MQ=255) gcTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCg > 1:1970075/1‑54 (MQ=255) gcTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGtt > 1:734468/1‑71 (MQ=255) gtggCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCg < 1:2740711/50‑1 (MQ=255) gtggCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCg < 1:3795902/50‑1 (MQ=255) tggCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgt < 1:2180698/50‑1 (MQ=255) tggCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCa > 1:1010978/1‑63 (MQ=255) gCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCcgccgc > 1:2756541/1‑71 (MQ=255) gCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCcgccgc > 1:1545887/1‑71 (MQ=255) gAAGGCACCCTGACGACAATCTACGGTATTCTGGTGtt < 1:3542067/38‑1 (MQ=255) gAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGt < 1:185269/61‑1 (MQ=255) aaGGCACCCTGACGACAATCTACGGTATTCTGGTGttttt < 1:696710/40‑1 (MQ=255) ggCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTAc > 1:2994862/1‑40 (MQ=255) ggCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCa > 1:449523/1‑71 (MQ=255) ggCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCa > 1:1441488/1‑71 (MQ=255) cACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCgt > 1:3252134/1‑41 (MQ=255) aCCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGa < 1:292718/71‑1 (MQ=255) aCCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGa < 1:1101593/71‑1 (MQ=255) cccTGACGACAATCTACGGTATTCTGGTGTTTTTa < 1:2917977/35‑1 (MQ=255) cccTGACGACAATCTACGGTATTCTGGTGTTTTTa < 1:3779742/35‑1 (MQ=255) cccTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCg < 1:2659654/47‑1 (MQ=255) cccTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGt < 1:2281168/54‑1 (MQ=255) | ATCTTTACGTTCGTGCTGGGGCTGCTGGATAAAATGTTAGGCTGGCAACGCGCTGGTGGCGAAGGCACCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGA > minE/2087599‑2087735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |