BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,093,700	A→G	7.3%	V384V (GTT→GTC)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,093,700	0	A	G	7.3%	311.6 / 13.0	110	V384V (GTT→GTC)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand): ref base A (55/47); new base G (5/3); total (60/50)
Fisher's exact test for biased strand distribution p-value = 7.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 113 total aligned reads displayed.

GCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAC  >  minE/2093632‑2093770

   gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                                                   <  1:3185210/71‑1 (MQ=255)

   gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                                                   <  1:1426622/71‑1 (MQ=255)

     gTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                                                 >  1:2952892/1‑71 (MQ=255)

        tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                                                 >  1:3045498/1‑68 (MQ=255)

        tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                               >  1:584437/1‑70 (MQ=255)

        tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                               >  1:817215/1‑70 (MQ=255)

         aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGc                                                              <  1:1263516/70‑1 (MQ=255)

          cTGCTTTCAAGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                            <  1:2499662/71‑1 (MQ=255)

            gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                          >  1:1794691/1‑71 (MQ=255)

            gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                          >  1:489675/1‑71 (MQ=255)

             cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAgg                                                           <  1:451817/69‑1 (MQ=255)

                  aCGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                                                        <  1:316715/51‑1 (MQ=255)

                  aCGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                    <  1:425164/71‑1 (MQ=255)

                    ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                    >  1:3905217/1‑69 (MQ=255)

                    ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                  <  1:1878870/71‑1 (MQ=255)

                     gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                 >  1:1202718/1‑71 (MQ=255)

                       aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                               <  1:2109303/71‑1 (MQ=255)

                       aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                               <  1:113911/71‑1 (MQ=255)

                       aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                               >  1:4134366/1‑71 (MQ=255)

                        ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAAc                                                                      <  1:2392637/47‑1 (MQ=255)

                        ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                               <  1:1174990/54‑1 (MQ=255)

                        ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                               <  1:566600/54‑1 (MQ=255)

                        ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt                                              <  1:2191213/71‑1 (MQ=255)

                        ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                               <  1:2911664/70‑1 (MQ=255)

                          aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCa                                                             >  1:1910428/1‑54 (MQ=255)

                          aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                             <  1:3972130/70‑1 (MQ=255)

                          aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                            >  1:3120283/1‑71 (MQ=255)

                           aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATg                                           >  1:4009992/1‑71 (MQ=255)

                            tGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                                       <  1:2023941/58‑1 (MQ=255)

                             ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAgac                                                     >  1:640222/1‑57 (MQ=255)

                              gcgttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTc                                        >  1:69646/4‑71 (MQ=255)

                              gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                                                 >  1:1780937/1‑46 (MQ=255)

                              gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                                        <  1:2627347/71‑1 (MQ=255)

                               cATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                                        >  1:1579481/1‑70 (MQ=255)

                                 ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                                                   <  1:498529/41‑1 (MQ=255)

                                 ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                                                   <  1:1551114/41‑1 (MQ=255)

                                 ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCt                                         <  1:2258390/67‑1 (MQ=255)

                                 ttACGTTCAATGCACGGGATTTGTACCTGGCCGGCAACCgg                                                                   <  1:713423/41‑1 (MQ=255)

                                   aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                                                 >  1:3329458/1‑41 (MQ=255)

                                   aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCa                                                             >  1:3451957/1‑45 (MQ=255)

                                   aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                            >  1:57597/1‑46 (MQ=255)

                                   aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                            >  1:173999/1‑46 (MQ=255)

                                   aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                   >  1:699692/1‑71 (MQ=255)

                                    cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                  >  1:3920832/1‑55 (MQ=255)

                                    cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                   >  1:1117471/1‑70 (MQ=255)

                                      ttCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATg                                           >  1:858876/1‑60 (MQ=255)

                                      ttCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATg                                           >  1:2011541/1‑60 (MQ=255)

                                      ttCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGa                                <  1:2503273/71‑1 (MQ=255)

                                       tCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt                               >  1:2310371/1‑71 (MQ=255)

                                        cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                 >  1:1413031/1‑52 (MQ=255)

                                        cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                              >  1:2129286/1‑71 (MQ=255)

                                        cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                              >  1:4093872/1‑71 (MQ=255)

                                           tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                           >  1:2888998/1‑71 (MQ=255)

                                           tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                           <  1:1536656/71‑1 (MQ=255)

                                           tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                           >  1:2745009/1‑71 (MQ=255)

                                           tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                            >  1:1670312/1‑70 (MQ=255)

                                             cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                               >  1:2691534/1‑49 (MQ=255)

                                             cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                         >  1:369025/1‑71 (MQ=255)

                                             cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                         >  1:3242848/1‑71 (MQ=255)

                                             cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                         >  1:2305667/1‑71 (MQ=255)

                                             cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccg                          <  1:397361/70‑1 (MQ=255)

                                              aCGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgcc                        >  1:3321422/1‑71 (MQ=255)

                                               cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg                       <  1:2485952/71‑1 (MQ=255)

                                               cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg                       >  1:3809978/1‑71 (MQ=255)

                                                 ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCAt                                     >  1:2908358/1‑55 (MQ=255)

                                                 ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                     <  1:859819/71‑1 (MQ=255)

                                                 ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                     <  1:1040543/71‑1 (MQ=255)

                                                 ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                     <  1:1789189/71‑1 (MQ=255)

                                                 ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                     >  1:2310296/1‑71 (MQ=255)

                                                  gATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                    >  1:2266082/1‑71 (MQ=255)

                                                   aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                               <  1:226628/43‑1 (MQ=255)

                                                   aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                   <  1:392822/71‑1 (MQ=255)

                                                   aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                   <  1:2655062/71‑1 (MQ=255)

                                                    tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                   <  1:1633957/70‑1 (MQ=255)

                                                     ctGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCcgccgc                      <  1:101106/65‑1 (MQ=12)

                                                      tGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCcgccgc                      <  1:766565/65‑1 (MQ=12)

                                                      tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                 >  1:1869080/1‑38 (MQ=255)

                                                      tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                            >  1:2712877/1‑59 (MQ=255)

                                                      tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                   >  1:2051032/1‑68 (MQ=255)

                                                      tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                >  1:3514626/1‑71 (MQ=255)

                                                       gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAgg                                                >  1:984291/1‑38 (MQ=12)

                                                       gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa                >  1:1487172/1‑70 (MQ=255)

                                                       gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                >  1:3698576/1‑70 (MQ=255)

                                                        tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCcgccgc                      <  1:3578752/63‑1 (MQ=255)

                                                        tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                 <  1:3268612/68‑1 (MQ=255)

                                                        tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                 <  1:769223/68‑1 (MQ=255)

                                                        tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                 <  1:3447487/68‑1 (MQ=255)

                                                        tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATa              <  1:2384791/71‑1 (MQ=255)

                                                         aCCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                   >  1:3439989/1‑65 (MQ=255)

                                                          ccTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAcc            >  1:570289/1‑71 (MQ=255)

                                                           cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                               <  1:31938/35‑1 (MQ=255)

                                                             gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                             >  1:53049/1‑35 (MQ=255)

                                                             gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                 >  1:503312/1‑63 (MQ=255)

                                                             gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                 >  1:1330896/1‑63 (MQ=255)

                                                              tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgcg        >  1:1264869/1‑71 (MQ=255)

                                                                 ggCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                            <  1:225504/48‑1 (MQ=255)

                                                                 ggCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCg     <  1:2758217/71‑1 (MQ=255)

                                                                  gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                     <  1:3575496/54‑1 (MQ=255)

                                                                   cAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGAGATTTc                            >  1:4093129/1‑46 (MQ=255)

                                                                    aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc  >  1:2078979/1‑71 (MQ=255)

GCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAC  >  minE/2093632‑2093770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`