BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,093,706	A→G	7.3%	D382D (GAT→GAC)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,093,706	0	A	G	7.3%	308.5 / 13.2	109	D382D (GAT→GAC)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand): ref base A (58/43); new base G (5/3); total (63/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 111 total aligned reads displayed.

CGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGC  >  minE/2093636‑2093773

cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa                                                                     >  1:1297031/1‑71 (MQ=255)

    tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                                                    >  1:3045498/1‑68 (MQ=255)

    tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                                  >  1:584437/1‑70 (MQ=255)

    tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                                  >  1:817215/1‑70 (MQ=255)

     aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGc                                                                 <  1:1263516/70‑1 (MQ=255)

      cTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAACTGTCCGGCAACCGGATCGCa                                                                <  1:517332/70‑1 (MQ=255)

      cTGCTTTCAAGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                               <  1:2499662/71‑1 (MQ=255)

        gCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                                             >  1:489675/1‑71 (MQ=255)

         cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAgg                                                              <  1:451817/69‑1 (MQ=255)

              aCGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                       <  1:425164/71‑1 (MQ=255)

                ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                       >  1:3905217/1‑69 (MQ=255)

                ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                     <  1:1878870/71‑1 (MQ=255)

                 gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                    >  1:1202718/1‑71 (MQ=255)

                   aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                  >  1:4134366/1‑71 (MQ=255)

                   aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                  <  1:113911/71‑1 (MQ=255)

                   aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                  <  1:2109303/71‑1 (MQ=255)

                    ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                                  <  1:566600/54‑1 (MQ=255)

                    ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                                                  <  1:1174990/54‑1 (MQ=255)

                    ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt                                                 <  1:2191213/71‑1 (MQ=255)

                    ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                  <  1:2911664/70‑1 (MQ=255)

                      aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCa                                                                >  1:1910428/1‑54 (MQ=255)

                      aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                <  1:3972130/70‑1 (MQ=255)

                      aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                               >  1:3337251/1‑71 (MQ=255)

                      aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                               >  1:2390965/1‑71 (MQ=255)

                      aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                               >  1:3120283/1‑71 (MQ=255)

                       aTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAg                                                          <  1:2715810/59‑1 (MQ=255)

                       aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATg                                              >  1:4009992/1‑71 (MQ=255)

                        tGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                                          <  1:2023941/58‑1 (MQ=255)

                         ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAgac                                                        >  1:640222/1‑57 (MQ=255)

                          gcgttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTc                                           >  1:69646/4‑71 (MQ=255)

                          gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                                                    >  1:1780937/1‑46 (MQ=255)

                          gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                                           <  1:2627347/71‑1 (MQ=255)

                           cATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                                           >  1:1579481/1‑70 (MQ=255)

                             ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCt                                            <  1:2258390/67‑1 (MQ=255)

                               aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                                                    >  1:3329458/1‑41 (MQ=255)

                               aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCa                                                                >  1:3451957/1‑45 (MQ=255)

                               aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                                               >  1:173999/1‑46 (MQ=255)

                               aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                      >  1:699692/1‑71 (MQ=255)

                               aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                      >  1:2228876/1‑69 (MQ=255)

                                cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                     >  1:3920832/1‑55 (MQ=255)

                                cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                      >  1:1117471/1‑70 (MQ=255)

                                  ttCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATg                                              >  1:2011541/1‑60 (MQ=255)

                                  ttCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATg                                              >  1:858876/1‑60 (MQ=255)

                                  ttCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGa                                   <  1:2503273/71‑1 (MQ=255)

                                    cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                 >  1:4093872/1‑71 (MQ=255)

                                       tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                              <  1:1536656/71‑1 (MQ=255)

                                       tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                              >  1:2888998/1‑71 (MQ=255)

                                       tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                              >  1:2745009/1‑71 (MQ=255)

                                       tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                               >  1:1670312/1‑70 (MQ=255)

                                         cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                  >  1:2691534/1‑49 (MQ=255)

                                         cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                            >  1:3242848/1‑71 (MQ=255)

                                         cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                            >  1:369025/1‑71 (MQ=255)

                                         cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                            >  1:2305667/1‑71 (MQ=255)

                                         cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccg                             <  1:397361/70‑1 (MQ=255)

                                          aCGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgcc                           >  1:3321422/1‑71 (MQ=255)

                                           cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg                          <  1:2485952/71‑1 (MQ=255)

                                             ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCAt                                        >  1:2908358/1‑55 (MQ=255)

                                             ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                        <  1:859819/71‑1 (MQ=255)

                                             ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                        <  1:1040543/71‑1 (MQ=255)

                                             ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                        <  1:1789189/71‑1 (MQ=255)

                                             ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                        >  1:2310296/1‑71 (MQ=255)

                                              gATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                       >  1:2266082/1‑71 (MQ=255)

                                               aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                  <  1:226628/43‑1 (MQ=255)

                                               aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                  <  1:3803092/43‑1 (MQ=255)

                                               aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                      <  1:3079567/71‑1 (MQ=255)

                                               aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                      <  1:392822/71‑1 (MQ=255)

                                               aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                      <  1:2655062/71‑1 (MQ=255)

                                                tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                      <  1:1633957/70‑1 (MQ=255)

                                                 ctGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCcgccgc                         <  1:101106/65‑1 (MQ=12)

                                                  tGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCcgccgc                         <  1:766565/65‑1 (MQ=12)

                                                  tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                    >  1:1869080/1‑38 (MQ=255)

                                                  tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                               >  1:4073972/1‑43 (MQ=255)

                                                  tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                               >  1:2712877/1‑59 (MQ=255)

                                                  tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                      >  1:2051032/1‑68 (MQ=255)

                                                  tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                   >  1:3514626/1‑71 (MQ=255)

                                                   gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa                   >  1:1487172/1‑70 (MQ=255)

                                                   gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                   >  1:3698576/1‑70 (MQ=255)

                                                    tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCcgccgc                         <  1:3578752/63‑1 (MQ=255)

                                                    tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                    <  1:3268612/68‑1 (MQ=255)

                                                    tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                    <  1:1918185/68‑1 (MQ=255)

                                                    tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                    <  1:3447487/68‑1 (MQ=255)

                                                    tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                    <  1:769223/68‑1 (MQ=255)

                                                    tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATa                 <  1:2384791/71‑1 (MQ=255)

                                                     aCCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                      >  1:3439989/1‑65 (MQ=255)

                                                      ccTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAcc               >  1:570289/1‑71 (MQ=255)

                                                       cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                  <  1:31938/35‑1 (MQ=255)

                                                         gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                >  1:53049/1‑35 (MQ=255)

                                                         gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                    >  1:1330896/1‑63 (MQ=255)

                                                         gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                    >  1:503312/1‑63 (MQ=255)

                                                          tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgcg           >  1:1264869/1‑71 (MQ=255)

                                                             ggCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                               <  1:225504/48‑1 (MQ=255)

                                                             ggCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCg        <  1:2758217/71‑1 (MQ=255)

                                                              gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                        <  1:3575496/54‑1 (MQ=255)

                                                               cAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGAGATTTc                               >  1:4093129/1‑46 (MQ=255)

                                                                aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc     >  1:2078979/1‑71 (MQ=255)

                                                                 aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                  <  1:2454734/57‑1 (MQ=255)

                                                                  ccGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCAGCCGCATTGCATACCTGCGCCGGACTg   >  1:2219078/1‑71 (MQ=255)

                                                                   cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc         <  1:2440865/64‑1 (MQ=255)

                                                                    ggATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                      <  1:2891229/50‑1 (MQ=255)

                                                                      aTCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                    >  1:1735763/1‑50 (MQ=255)

CGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGC  >  minE/2093636‑2093773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`