BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,093,718	C→A	7.7%	G378G (GGG→GGT)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,093,718	0	C	A	7.7%	333.8 / 6.9	101	G378G (GGG→GGT)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand): ref base C (53/40); new base A (5/3); total (58/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

ACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTT  >  minE/2093650‑2093785

aCGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                                   <  1:425164/71‑1 (MQ=255)

  ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                                                   >  1:3905217/1‑69 (MQ=255)

  ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                                 <  1:1878870/71‑1 (MQ=255)

   gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                                >  1:1202718/1‑71 (MQ=255)

     aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                              <  1:2109303/71‑1 (MQ=255)

     aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                              <  1:113911/71‑1 (MQ=255)

     aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                              >  1:4134366/1‑71 (MQ=255)

      ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt                                                             <  1:2191213/71‑1 (MQ=255)

      ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                              <  1:2911664/70‑1 (MQ=255)

        aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                            <  1:3972130/70‑1 (MQ=255)

        aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                           >  1:3337251/1‑71 (MQ=255)

        aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                           >  1:3120283/1‑71 (MQ=255)

        aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                           >  1:2390965/1‑71 (MQ=255)

         aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATg                                                          >  1:4009992/1‑71 (MQ=255)

            gcgttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTc                                                       >  1:69646/4‑71 (MQ=255)

            gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                                                       <  1:2627347/71‑1 (MQ=255)

             cATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                                                       >  1:1579481/1‑70 (MQ=255)

               ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCt                                                        <  1:2258390/67‑1 (MQ=255)

                 aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                  >  1:699692/1‑71 (MQ=255)

                 aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                                  >  1:2228876/1‑69 (MQ=255)

                  cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                                 >  1:3920832/1‑55 (MQ=255)

                  cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                  >  1:1117471/1‑70 (MQ=255)

                    ttCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATg                                                          >  1:2011541/1‑60 (MQ=255)

                    ttCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATg                                                          >  1:858876/1‑60 (MQ=255)

                    ttCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGa                                               <  1:2503273/71‑1 (MQ=255)

                     tCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt                                              >  1:2310371/1‑71 (MQ=255)

                      cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                                >  1:1413031/1‑52 (MQ=255)

                      cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                             >  1:2129286/1‑71 (MQ=255)

                      cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                             >  1:4093872/1‑71 (MQ=255)

                         tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                          >  1:2745009/1‑71 (MQ=255)

                         tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                          >  1:2888998/1‑71 (MQ=255)

                         tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                          <  1:1536656/71‑1 (MQ=255)

                         tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                                           >  1:1670312/1‑70 (MQ=255)

                           cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                              >  1:2691534/1‑49 (MQ=255)

                           cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                        >  1:3242848/1‑71 (MQ=255)

                           cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                        >  1:369025/1‑71 (MQ=255)

                           cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccg                                         <  1:397361/70‑1 (MQ=255)

                            aCGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgcc                                       >  1:3321422/1‑71 (MQ=255)

                             cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg                                      <  1:2485952/71‑1 (MQ=255)

                             cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg                                      >  1:3809978/1‑71 (MQ=255)

                               ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCAt                                                    >  1:2908358/1‑55 (MQ=255)

                               ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                                    <  1:1040543/71‑1 (MQ=255)

                               ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                                    <  1:859819/71‑1 (MQ=255)

                               ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                                    <  1:1789189/71‑1 (MQ=255)

                               ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                                    >  1:2310296/1‑71 (MQ=255)

                                gATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                   >  1:2266082/1‑71 (MQ=255)

                                 aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                              <  1:226628/43‑1 (MQ=255)

                                 aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                              <  1:3803092/43‑1 (MQ=255)

                                 aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                  <  1:392822/71‑1 (MQ=255)

                                 aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                  <  1:3079567/71‑1 (MQ=255)

                                 aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                  <  1:2655062/71‑1 (MQ=255)

                                  tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                  <  1:1633957/70‑1 (MQ=255)

                                   ctGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCcgccgc                                     <  1:101106/65‑1 (MQ=12)

                                    tGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCcgccgc                                     <  1:766565/65‑1 (MQ=12)

                                    tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                                >  1:1869080/1‑38 (MQ=255)

                                    tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                           >  1:4073972/1‑43 (MQ=255)

                                    tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                                           >  1:2712877/1‑59 (MQ=255)

                                    tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                  >  1:2051032/1‑68 (MQ=255)

                                    tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                               >  1:3514626/1‑71 (MQ=255)

                                     gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa                               >  1:1487172/1‑70 (MQ=255)

                                     gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                               >  1:3698576/1‑70 (MQ=255)

                                      tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCcgccgc                                     <  1:3578752/63‑1 (MQ=255)

                                      tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                <  1:1918185/68‑1 (MQ=255)

                                      tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                <  1:3268612/68‑1 (MQ=255)

                                      tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                <  1:769223/68‑1 (MQ=255)

                                      tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATa                             <  1:2384791/71‑1 (MQ=255)

                                       aCCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                  >  1:3439989/1‑65 (MQ=255)

                                        ccTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAcc                           >  1:570289/1‑71 (MQ=255)

                                         cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                              <  1:31938/35‑1 (MQ=255)

                                           gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                            >  1:53049/1‑35 (MQ=255)

                                           gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                >  1:1330896/1‑63 (MQ=255)

                                           gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                >  1:503312/1‑63 (MQ=255)

                                            tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgcg                       >  1:1264869/1‑71 (MQ=255)

                                               ggCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                                           <  1:225504/48‑1 (MQ=255)

                                               ggCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCg                    <  1:2758217/71‑1 (MQ=255)

                                                gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                                    <  1:3575496/54‑1 (MQ=255)

                                                 cAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGAGATTTc                                           >  1:4093129/1‑46 (MQ=255)

                                                  aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc                 >  1:2078979/1‑71 (MQ=255)

                                                   aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                              <  1:2454734/57‑1 (MQ=255)

                                                    ccGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATATCTGCGCCGGACTg               >  1:2669204/1‑71 (MQ=255)

                                                    ccGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCAGCCGCATTGCATACCTGCGCCGGACTg               >  1:2219078/1‑71 (MQ=255)

                                                     cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                  <  1:4117087/51‑1 (MQ=255)

                                                     cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc                     <  1:2440865/64‑1 (MQ=255)

                                                     cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTgc              >  1:335540/1‑71 (MQ=255)

                                                      ggATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                  <  1:2891229/50‑1 (MQ=255)

                                                        aTCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                >  1:1735763/1‑50 (MQ=255)

                                                          cGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                   >  1:2929045/1‑45 (MQ=255)

                                                          cGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                   >  1:829668/1‑45 (MQ=255)

                                                            cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag       >  1:3170170/1‑71 (MQ=255)

                                                            cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag       <  1:667957/71‑1 (MQ=255)

                                                               gTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                  <  1:3381146/41‑1 (MQ=255)

                                                               gTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                 <  1:1682587/42‑1 (MQ=255)

                                                               gTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtag    <  1:3128044/71‑1 (MQ=255)

                                                               gTCAGCCCAAGGTTATGCACCATCGCGATTTCcgccgc                                     >  1:3508017/1‑38 (MQ=255)

                                                               gTCAGACCGAGGTTGTGCTCCATGGCGATTTCcgccgcga                                   >  1:37820/1‑38 (MQ=255)

                                                                tCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtagt   >  1:693515/1‑71 (MQ=255)

                                                                 cAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtagt   >  1:3078577/1‑70 (MQ=255)

                                                                 cAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt  >  1:1853429/1‑71 (MQ=255)

                                                                 cAGACCGAGGTTGTGCTCCATGGCGATTTCcgccgc                                     <  1:1617252/36‑1 (MQ=9)

                                                                  aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt  <  1:2031998/70‑1 (MQ=255)

ACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTT  >  minE/2093650‑2093785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`