BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,093,721	A→G	7.9%	L377L (CTT→CTC)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,093,721	0	A	G	7.9%	284.5 / 12.7	101	L377L (CTT→CTC)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand): ref base A (53/40); new base G (5/3); total (58/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 105 total aligned reads displayed.

GGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTA  >  minE/2093652‑2093791

ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                                       <  1:1878870/71‑1 (MQ=255)

 gCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                                      >  1:1202718/1‑71 (MQ=255)

   aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                                    <  1:113911/71‑1 (MQ=255)

   aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                                    <  1:2109303/71‑1 (MQ=255)

   aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                                    >  1:4134366/1‑71 (MQ=255)

    ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGtt                                                                   <  1:2191213/71‑1 (MQ=255)

    ttAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                                    <  1:2911664/70‑1 (MQ=255)

      aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                 >  1:3337251/1‑71 (MQ=255)

      aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                 >  1:3120283/1‑71 (MQ=255)

      aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                 >  1:2390965/1‑71 (MQ=255)

       aTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATg                                                                >  1:4009992/1‑71 (MQ=255)

          gcgttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTc                                                             >  1:69646/4‑71 (MQ=255)

          gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                                                             <  1:2627347/71‑1 (MQ=255)

           cATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTc                                                             >  1:1579481/1‑70 (MQ=255)

             ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCt                                                              <  1:2258390/67‑1 (MQ=255)

               aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                        >  1:699692/1‑71 (MQ=255)

               aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                                        >  1:2228876/1‑69 (MQ=255)

                cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                                       >  1:3920832/1‑55 (MQ=255)

                cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                        >  1:1117471/1‑70 (MQ=255)

                  ttCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATg                                                                >  1:858876/1‑60 (MQ=255)

                  ttCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATg                                                                >  1:2011541/1‑60 (MQ=255)

                  ttCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGa                                                     <  1:2503273/71‑1 (MQ=255)

                   tCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt                                                    >  1:2310371/1‑71 (MQ=255)

                    cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                                      >  1:1413031/1‑52 (MQ=255)

                    cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                                   >  1:2129286/1‑71 (MQ=255)

                    cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                                   >  1:4093872/1‑71 (MQ=255)

                       tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                                <  1:1536656/71‑1 (MQ=255)

                       tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                                >  1:2888998/1‑71 (MQ=255)

                       tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                                >  1:2745009/1‑71 (MQ=255)

                       tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                                                 >  1:1670312/1‑70 (MQ=255)

                         cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                                    >  1:2691534/1‑49 (MQ=255)

                         cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                              >  1:369025/1‑71 (MQ=255)

                         cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                              >  1:2305667/1‑71 (MQ=255)

                         cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccg                                               <  1:397361/70‑1 (MQ=255)

                          aCGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgcc                                             >  1:3321422/1‑71 (MQ=255)

                           cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg                                            >  1:3809978/1‑71 (MQ=255)

                             ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCAt                                                          >  1:2908358/1‑55 (MQ=255)

                             ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                                          <  1:859819/71‑1 (MQ=255)

                             ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                                          <  1:1040543/71‑1 (MQ=255)

                             ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                                          <  1:1789189/71‑1 (MQ=255)

                             ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                                          >  1:2310296/1‑71 (MQ=255)

                              gATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                         >  1:2266082/1‑71 (MQ=255)

                               aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                                    <  1:3803092/43‑1 (MQ=255)

                               aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                                    <  1:226628/43‑1 (MQ=255)

                               aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                        <  1:392822/71‑1 (MQ=255)

                               aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                        <  1:2655062/71‑1 (MQ=255)

                               aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                        <  1:3079567/71‑1 (MQ=255)

                                tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                        <  1:1633957/70‑1 (MQ=255)

                                 ctGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCcgccgc                                           <  1:101106/65‑1 (MQ=12)

                                  tGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCcgccgc                                           <  1:766565/65‑1 (MQ=12)

                                  tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAg                                                                      >  1:1869080/1‑38 (MQ=255)

                                  tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                 >  1:4073972/1‑43 (MQ=255)

                                  tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                                                 >  1:2712877/1‑59 (MQ=255)

                                  tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                        >  1:2051032/1‑68 (MQ=255)

                                  tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                                     >  1:3514626/1‑71 (MQ=255)

                                   gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa                                     >  1:1487172/1‑70 (MQ=255)

                                   gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                                     >  1:3698576/1‑70 (MQ=255)

                                    tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCcgccgc                                           <  1:3578752/63‑1 (MQ=255)

                                    tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                      <  1:769223/68‑1 (MQ=255)

                                    tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                      <  1:3268612/68‑1 (MQ=255)

                                    tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                      <  1:3447487/68‑1 (MQ=255)

                                    tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATa                                   <  1:2384791/71‑1 (MQ=255)

                                     aCCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                        >  1:3439989/1‑65 (MQ=255)

                                      ccTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAcc                                 >  1:570289/1‑71 (MQ=255)

                                       cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                                    <  1:31938/35‑1 (MQ=255)

                                         gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                  >  1:53049/1‑35 (MQ=255)

                                         gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                      >  1:1330896/1‑63 (MQ=255)

                                         gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                      >  1:503312/1‑63 (MQ=255)

                                          tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgcg                             >  1:1264869/1‑71 (MQ=255)

                                             ggCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                                                 <  1:225504/48‑1 (MQ=255)

                                             ggCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCg                          <  1:2758217/71‑1 (MQ=255)

                                              gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                                          <  1:3575496/54‑1 (MQ=255)

                                               cAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGAGATTTc                                                 >  1:4093129/1‑46 (MQ=255)

                                                aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc                       >  1:2078979/1‑71 (MQ=255)

                                                 aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                    <  1:2454734/57‑1 (MQ=255)

                                                  ccGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATATCTGCGCCGGACTg                     >  1:2669204/1‑71 (MQ=255)

                                                  ccGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCAGCCGCATTGCATACCTGCGCCGGACTg                     >  1:2219078/1‑71 (MQ=255)

                                                   cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                        <  1:4117087/51‑1 (MQ=255)

                                                   cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc                           <  1:2440865/64‑1 (MQ=255)

                                                   cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTgc                    >  1:335540/1‑71 (MQ=255)

                                                    ggATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                        <  1:2891229/50‑1 (MQ=255)

                                                      aTCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                      >  1:1735763/1‑50 (MQ=255)

                                                        cGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                         >  1:829668/1‑45 (MQ=255)

                                                        cGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                         >  1:2929045/1‑45 (MQ=255)

                                                          cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag             >  1:3170170/1‑71 (MQ=255)

                                                          cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag             <  1:667957/71‑1 (MQ=255)

                                                             gTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                        <  1:3381146/41‑1 (MQ=255)

                                                             gTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                       <  1:1682587/42‑1 (MQ=255)

                                                             gTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtag          <  1:3128044/71‑1 (MQ=255)

                                                             gTCAGCCCAAGGTTATGCACCATCGCGATTTCcgccgc                                           >  1:3508017/1‑38 (MQ=255)

                                                             gTCAGACCGAGGTTGTGCTCCATGGCGATTTCcgccgcga                                         >  1:37820/1‑38 (MQ=255)

                                                              tCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtagt         >  1:693515/1‑71 (MQ=255)

                                                               cAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtagt         >  1:3078577/1‑70 (MQ=255)

                                                               cAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt        >  1:1853429/1‑71 (MQ=255)

                                                               cAGACCGAGGTTGTGCTCCATGGCGATTTCcgccgc                                           <  1:1617252/36‑1 (MQ=9)

                                                                aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt        <  1:2031998/70‑1 (MQ=255)

                                                                    cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAcc                                 >  1:3815246/1‑41 (MQ=255)

                                                                    cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGtt   <  1:3653623/71‑1 (MQ=255)

                                                                     aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa  <  1:791864/71‑1 (MQ=255)

                                                                     aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa  <  1:2616165/71‑1 (MQ=255)

GGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTA  >  minE/2093652‑2093791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`