Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,093,736 C→G 6.1% A372A (GCG→GCC)  tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,093,7360CG 6.1% 251.7 / 3.5 80A372A (GCG→GCC) tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base C (38/37);  new base G (2/3);  total (40/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCC  >  minE/2093667‑2093805
                                                                     |                                                                     
aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                                      >  1:699692/1‑71 (MQ=255)
 cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                                      >  1:1117471/1‑70 (MQ=255)
   ttCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGa                                                                   <  1:2503273/71‑1 (MQ=255)
    tCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt                                                                  >  1:2310371/1‑71 (MQ=255)
     cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                                                 >  1:2129286/1‑71 (MQ=255)
     cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                                                 >  1:4093872/1‑71 (MQ=255)
        tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                                              <  1:1536656/71‑1 (MQ=255)
        tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                                              >  1:2888998/1‑71 (MQ=255)
        tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                                              >  1:2745009/1‑71 (MQ=255)
        tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                                                               >  1:1670312/1‑70 (MQ=255)
          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                                            >  1:369025/1‑71 (MQ=255)
          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                                            >  1:3242848/1‑71 (MQ=255)
          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                                            >  1:2305667/1‑71 (MQ=255)
          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccg                                                             <  1:397361/70‑1 (MQ=255)
           aCGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgcc                                                           >  1:3321422/1‑71 (MQ=255)
            cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg                                                          >  1:3809978/1‑71 (MQ=255)
            cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg                                                          <  1:2485952/71‑1 (MQ=255)
              ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                                                        >  1:2310296/1‑71 (MQ=255)
              ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                                                        <  1:859819/71‑1 (MQ=255)
              ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                                                        <  1:1040543/71‑1 (MQ=255)
              ggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                                                        <  1:1789189/71‑1 (MQ=255)
               gATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                                       >  1:2266082/1‑71 (MQ=255)
                aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                                      <  1:3079567/71‑1 (MQ=255)
                aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                                      <  1:2655062/71‑1 (MQ=255)
                aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                                      <  1:392822/71‑1 (MQ=255)
                 tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                                      <  1:1633957/70‑1 (MQ=255)
                  ctGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCcgccgc                                                         <  1:101106/65‑1 (MQ=12)
                   tGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCcgccgc                                                         <  1:766565/65‑1 (MQ=12)
                   tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                                                               >  1:2712877/1‑59 (MQ=255)
                   tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                                      >  1:2051032/1‑68 (MQ=255)
                   tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                                                   >  1:3514626/1‑71 (MQ=255)
                    gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa                                                   >  1:1487172/1‑70 (MQ=255)
                    gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                                                   >  1:3698576/1‑70 (MQ=255)
                     tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCcgccgc                                                         <  1:3578752/63‑1 (MQ=255)
                     tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                                    <  1:3268612/68‑1 (MQ=255)
                     tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                                    <  1:1918185/68‑1 (MQ=255)
                     tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                                    <  1:769223/68‑1 (MQ=255)
                     tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                                    <  1:3447487/68‑1 (MQ=255)
                     tACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATa                                                 <  1:2384791/71‑1 (MQ=255)
                      aCCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                                      >  1:3439989/1‑65 (MQ=255)
                       ccTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAcc                                               >  1:570289/1‑71 (MQ=255)
                          gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                                    >  1:1330896/1‑63 (MQ=255)
                          gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                                    >  1:503312/1‑63 (MQ=255)
                           tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgcg                                           >  1:1264869/1‑71 (MQ=255)
                              ggCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTc                                                               <  1:225504/48‑1 (MQ=255)
                              ggCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCg                                        <  1:2758217/71‑1 (MQ=255)
                               gCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCa                                                        <  1:3575496/54‑1 (MQ=255)
                                cAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGAGATTTc                                                               >  1:4093129/1‑46 (MQ=255)
                                 aaCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGAc                                     >  1:2078979/1‑71 (MQ=255)
                                  aCCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                                  <  1:2454734/57‑1 (MQ=255)
                                   ccGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATATCTGCGCCGGACTg                                   >  1:2669204/1‑71 (MQ=255)
                                   ccGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCAGCCGCATTGCATACCTGCGCCGGACTg                                   >  1:2219078/1‑71 (MQ=255)
                                    cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                                      <  1:4117087/51‑1 (MQ=255)
                                    cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc                                         <  1:2440865/64‑1 (MQ=255)
                                    cGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTgc                                  >  1:335540/1‑71 (MQ=255)
                                     ggATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                                      <  1:2891229/50‑1 (MQ=255)
                                       aTCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGc                                                    >  1:1735763/1‑50 (MQ=255)
                                         cGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                                       >  1:829668/1‑45 (MQ=255)
                                         cGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                                       >  1:2929045/1‑45 (MQ=255)
                                           cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag                           >  1:3170170/1‑71 (MQ=255)
                                           cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag                           <  1:667957/71‑1 (MQ=255)
                                              gTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAtt                                                      <  1:3381146/41‑1 (MQ=255)
                                              gTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                                     <  1:1682587/42‑1 (MQ=255)
                                              gTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtag                        <  1:3128044/71‑1 (MQ=255)
                                              gTCAGCCCAAGGTTATGCACCATCGCGATTTCcgccgc                                                         >  1:3508017/1‑38 (MQ=255)
                                              gTCAGACCGAGGTTGTGCTCCATGGCGATTTCcgccgcga                                                       >  1:37820/1‑38 (MQ=255)
                                               tCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtagt                       >  1:693515/1‑71 (MQ=255)
                                                cAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtagt                       >  1:3078577/1‑70 (MQ=255)
                                                cAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt                      >  1:1853429/1‑71 (MQ=255)
                                                cAGACCGAGGTTGTGCTCCATGGCGATTTCcgccgc                                                         <  1:1617252/36‑1 (MQ=9)
                                                 aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt                      <  1:2031998/70‑1 (MQ=255)
                                                     cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAcc                                               >  1:3815246/1‑41 (MQ=255)
                                                     cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGtt                 <  1:3653623/71‑1 (MQ=255)
                                                      aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa                <  1:2616165/71‑1 (MQ=255)
                                                      aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa                <  1:791864/71‑1 (MQ=255)
                                                       aGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTaa               <  1:4024254/71‑1 (MQ=255)
                                                       aGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTaa               <  1:791155/71‑1 (MQ=255)
                                                        ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCg                                        <  1:575663/45‑1 (MQ=255)
                                                            aTGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTGAGTTAACCCTg          >  1:1042683/1‑71 (MQ=255)
                                                               cTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccg       >  1:1852754/1‑71 (MQ=255)
                                                                    tCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGccc                             <  1:146142/44‑1 (MQ=255)
                                                                    tCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa                <  1:2834144/57‑1 (MQ=255)
                                                                     cgcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgcc  >  1:2207109/1‑70 (MQ=255)
                                                                     |                                                                     
ACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCC  >  minE/2093667‑2093805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑