Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,094,015	C→A	12.9%	G279G (GGG→GGT)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,094,015	0	C	A	12.9%	195.5 / 10.4	62	G279G (GGG→GGT)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base C (27/27);  new base A (3/5);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGG  >  minE/2093946‑2094083                                                                      |                                                                     cTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcc                                                                     >  1:3169939/1‑71 (MQ=255)    aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                                                                  >  1:3507484/1‑71 (MQ=255)    aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                                                                  >  1:1998707/1‑71 (MQ=255)         aataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCa                                                                >  1:30859/1‑68 (MQ=255)         aataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCt                                                             >  1:2630179/1‑71 (MQ=255)          ataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCt                                                             >  1:868048/1‑70 (MQ=255)          ataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTg                                                            >  1:1189465/1‑71 (MQ=255)                 aGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttc                                                     <  1:262743/71‑1 (MQ=255)                   tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTTCGTTttctt                                                   <  1:682388/71‑1 (MQ=255)                   tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCg                                                          <  1:4121814/64‑1 (MQ=255)                   tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                   <  1:291785/71‑1 (MQ=255)                   tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                   <  1:4096712/71‑1 (MQ=255)                    aCTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                   >  1:224093/1‑70 (MQ=255)                     cTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTctcttcg                                                 <  1:2716984/71‑2 (MQ=255)                     cTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTctcttcg                                                 <  1:327164/71‑2 (MQ=255)                     cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCa                                                 >  1:2928687/1‑71 (MQ=255)                     cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACAACGCGCCCGCCAGCTGCGTTTTCTTCa                                                 >  1:1124931/1‑71 (MQ=255)                       gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCAc                                                >  1:3214810/1‑70 (MQ=255)                       gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCAc                                                >  1:361529/1‑70 (MQ=255)                        ccGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACt                                               >  1:1250371/1‑70 (MQ=255)                        ccGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTa                                              <  1:139694/71‑1 (MQ=255)                         cGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTaa                                             <  1:2098593/71‑1 (MQ=255)                             ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                                         >  1:3465786/1‑71 (MQ=255)                             ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                                         >  1:3190474/1‑71 (MQ=255)                                 tGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAAcc                                           >  1:3695737/1‑65 (MQ=255)                                 tGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGTCAgc                                     <  1:424534/71‑1 (MQ=255)                                   ccGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtg                                    >  1:915853/1‑68 (MQ=255)                                   ccGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtg                                    >  1:2807830/1‑68 (MQ=255)                                    cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgca                                  <  1:1148017/71‑2 (MQ=255)                                    cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgca                                  <  1:3406803/71‑2 (MQ=255)                                    cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgc                                   >  1:3438212/1‑70 (MQ=255)                                     gcacgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg                                  >  1:2686903/1‑70 (MQ=255)                                        cgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACa                              >  1:3285942/1‑71 (MQ=255)                                         gcacCGTTAGTCGGCGCAGGCACCACGCGACCGCCAGCAGCGTTctct                                                    <  1:3950309/48‑1 (MQ=16)                                          cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATg                            <  1:2201729/71‑1 (MQ=255)                                          cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATg                            <  1:520067/71‑1 (MQ=255)                                           acCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGt                           <  1:3827713/71‑1 (MQ=255)                                            ccGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttct                                                    <  1:4082012/45‑1 (MQ=255)                                               ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                       >  1:1401770/1‑71 (MQ=255)                                               ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                       <  1:2463316/71‑1 (MQ=255)                                                tAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                       >  1:3042194/1‑70 (MQ=255)                                                 aGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTa                                              <  1:2193282/46‑1 (MQ=255)                                                  gTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATcc                     <  1:2943441/70‑1 (MQ=255)                                                  gTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa                    <  1:3478794/71‑1 (MQ=255)                                                   tCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGcc                                        >  1:4110362/1‑50 (MQ=255)                                                   tCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAg                   <  1:2559309/71‑1 (MQ=255)                                                      gTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                       <  1:2510980/64‑1 (MQ=255)                                                      gTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCg                <  1:3659028/71‑1 (MQ=255)                                                       tGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGa               >  1:1423232/1‑71 (MQ=255)                                                         ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGAt              <  1:1911099/70‑1 (MQ=255)                                                         ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATg             <  1:565092/71‑1 (MQ=255)                                                          cGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGt                           >  1:3777975/1‑56 (MQ=255)                                                           gTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGtt                          <  1:170599/56‑1 (MQ=255)                                                            ttACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGcc                                        >  1:3771245/1‑41 (MQ=255)                                                             ctccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACa          <  1:3673314/68‑1 (MQ=255)                                                              aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa                    >  1:3858945/1‑59 (MQ=255)                                                              aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa                    >  1:384980/1‑59 (MQ=255)                                                              aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt        >  1:3297635/1‑71 (MQ=255)                                                              aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCAAGCGCGTACATGTTGATCCAGTAGATGACAtt        >  1:3607588/1‑71 (MQ=255)                                                               ccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc       <  1:52666/71‑1 (MQ=255)                                                                cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTa                                <  1:246975/45‑1 (MQ=255)                                                                cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACa          <  1:3242432/67‑1 (MQ=255)                                                                 aCGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg                                  <  1:1901996/42‑1 (MQ=255)                                                                  cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGt                                 >  1:1547760/1‑42 (MQ=255)                                                                  cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTg                         >  1:646775/1‑50 (MQ=255)                                                                   gcgcCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc       >  1:629693/1‑67 (MQ=255)                                                                    cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACa                              >  1:2697589/1‑43 (MQ=255)                                                                    cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATc                      >  1:3685747/1‑51 (MQ=255)                                                                    cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg  <  1:1480860/71‑1 (MQ=255)                                                                    cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg  <  1:3147548/71‑1 (MQ=255)                                                                      |                                                                     CTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGG  >  minE/2093946‑2094083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑