Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,094,024	T→A	11.8%	A276A (GCA→GCT)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,094,024	0	T	A	11.8%	179.5 / 15.9	68	A276A (GCA→GCT)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base T (28/32);  new base A (3/5);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

AATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTA  >  minE/2093954‑2094090                                                                       |                                                                   aataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCt                                                                    >  1:2630179/1‑71 (MQ=255)  ataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCt                                                                    >  1:868048/1‑70 (MQ=255)  ataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTg                                                                   >  1:1189465/1‑71 (MQ=255)         aGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttc                                                            <  1:262743/71‑1 (MQ=255)           tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTTCGTTttctt                                                          <  1:682388/71‑1 (MQ=255)           tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCg                                                                 <  1:4121814/64‑1 (MQ=255)           tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                          <  1:291785/71‑1 (MQ=255)           tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                          <  1:4096712/71‑1 (MQ=255)            aCTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                          >  1:224093/1‑70 (MQ=255)             cTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTctcttcg                                                        <  1:2716984/71‑2 (MQ=255)             cTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTctcttcg                                                        <  1:327164/71‑2 (MQ=255)             cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCa                                                        >  1:2928687/1‑71 (MQ=255)             cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACAACGCGCCCGCCAGCTGCGTTTTCTTCa                                                        >  1:1124931/1‑71 (MQ=255)               gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCAc                                                       >  1:3214810/1‑70 (MQ=255)               gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCAc                                                       >  1:361529/1‑70 (MQ=255)                ccGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACt                                                      >  1:1250371/1‑70 (MQ=255)                ccGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTa                                                     <  1:139694/71‑1 (MQ=255)                 cGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTaa                                                    <  1:2098593/71‑1 (MQ=255)                     ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                                                >  1:3465786/1‑71 (MQ=255)                     ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                                                >  1:3190474/1‑71 (MQ=255)                         tGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAAcc                                                  >  1:3695737/1‑65 (MQ=255)                         tGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGTCAgc                                            <  1:424534/71‑1 (MQ=255)                           ccGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtg                                           >  1:2807830/1‑68 (MQ=255)                           ccGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtg                                           >  1:915853/1‑68 (MQ=255)                            cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgca                                         <  1:1148017/71‑2 (MQ=255)                            cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgca                                         <  1:3406803/71‑2 (MQ=255)                            cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgc                                          >  1:3438212/1‑70 (MQ=255)                             gcacgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg                                         >  1:2686903/1‑70 (MQ=255)                                cgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACa                                     >  1:3285942/1‑71 (MQ=255)                                 gcacCGTTAGTCGGCGCAGGCACCACGCGACCGCCAGCAGCGTTctct                                                           <  1:3950309/48‑1 (MQ=16)                                  cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATg                                   <  1:2201729/71‑1 (MQ=255)                                  cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATg                                   <  1:520067/71‑1 (MQ=255)                                   acCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGt                                  <  1:3827713/71‑1 (MQ=255)                                    ccGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttct                                                           <  1:4082012/45‑1 (MQ=255)                                       ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                              >  1:1401770/1‑71 (MQ=255)                                       ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                              <  1:2463316/71‑1 (MQ=255)                                        tAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                              >  1:3042194/1‑70 (MQ=255)                                         aGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTa                                                     <  1:2193282/46‑1 (MQ=255)                                          gTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATcc                            <  1:2943441/70‑1 (MQ=255)                                          gTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa                           <  1:3478794/71‑1 (MQ=255)                                           tCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGcc                                               >  1:4110362/1‑50 (MQ=255)                                           tCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAg                          <  1:2559309/71‑1 (MQ=255)                                              gTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                              <  1:2510980/64‑1 (MQ=255)                                              gTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCg                       <  1:3659028/71‑1 (MQ=255)                                               tGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGa                      >  1:1423232/1‑71 (MQ=255)                                                 ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGAt                     <  1:1911099/70‑1 (MQ=255)                                                 ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATg                    <  1:565092/71‑1 (MQ=255)                                                  cGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGt                                  >  1:3777975/1‑56 (MQ=255)                                                   gTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGtt                                 <  1:170599/56‑1 (MQ=255)                                                    ttACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGcc                                               >  1:3771245/1‑41 (MQ=255)                                                     ctccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACa                 <  1:3673314/68‑1 (MQ=255)                                                      aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa                           >  1:384980/1‑59 (MQ=255)                                                      aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa                           >  1:3858945/1‑59 (MQ=255)                                                      aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt               >  1:3297635/1‑71 (MQ=255)                                                      aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCAAGCGCGTACATGTTGATCCAGTAGATGACAtt               >  1:3607588/1‑71 (MQ=255)                                                       ccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc              <  1:52666/71‑1 (MQ=255)                                                        cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTa                                       <  1:246975/45‑1 (MQ=255)                                                        cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACa                 <  1:3242432/67‑1 (MQ=255)                                                         aCGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg                                         <  1:1901996/42‑1 (MQ=255)                                                          cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGt                                        >  1:1547760/1‑42 (MQ=255)                                                          cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTg                                >  1:646775/1‑50 (MQ=255)                                                           gcgcCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc              >  1:629693/1‑67 (MQ=255)                                                            cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACa                                     >  1:2697589/1‑43 (MQ=255)                                                            cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATc                             >  1:3685747/1‑51 (MQ=255)                                                            cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg         <  1:1480860/71‑1 (MQ=255)                                                            cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg         <  1:3147548/71‑1 (MQ=255)                                                                 gccAGCTGCGTTTTCTTCACTAACCGCCCGCGCGTACATGTTGa                               >  1:2108221/1‑44 (MQ=255)                                                                 gccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGt    <  1:865791/71‑1 (MQ=255)                                                                  ccATCTGCGTTTTCTTCACTAACCGCCAGCTCGTACATGTTGATCCAGTCGAt                     <  1:73069/53‑1 (MQ=38)                                                                      cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc              <  1:3855222/56‑1 (MQ=255)                                                                       tGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTa  >  1:3576235/1‑67 (MQ=255)                                                                       |                                                                   AATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTA  >  minE/2093954‑2094090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑