Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,094,030 T→C 8.2% E274E (GAA→GAG)  tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,094,0300TC 8.2% 171.2 / 6.6 61E274E (GAA→GAG) tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base T (30/26);  new base C (3/2);  total (33/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTAT  >  minE/2093962‑2094100
                                                                    |                                                                      
aGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttc                                                                      <  1:262743/71‑1 (MQ=255)
  tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTTCGTTttctt                                                                    <  1:682388/71‑1 (MQ=255)
  tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                                    <  1:4096712/71‑1 (MQ=255)
  tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                                    <  1:291785/71‑1 (MQ=255)
   aCTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                                    >  1:224093/1‑70 (MQ=255)
    cTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTctcttcg                                                                  <  1:2716984/71‑2 (MQ=255)
    cTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTctcttcg                                                                  <  1:327164/71‑2 (MQ=255)
    cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCa                                                                  >  1:2928687/1‑71 (MQ=255)
    cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACAACGCGCCCGCCAGCTGCGTTTTCTTCa                                                                  >  1:1124931/1‑71 (MQ=255)
      gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCAc                                                                 >  1:361529/1‑70 (MQ=255)
      gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCAc                                                                 >  1:3214810/1‑70 (MQ=255)
       ccGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACt                                                                >  1:1250371/1‑70 (MQ=255)
       ccGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTa                                                               <  1:139694/71‑1 (MQ=255)
        cGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTaa                                                              <  1:2098593/71‑1 (MQ=255)
            ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                                                          >  1:3465786/1‑71 (MQ=255)
            ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                                                          >  1:3190474/1‑71 (MQ=255)
                tGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAAcc                                                            >  1:3695737/1‑65 (MQ=255)
                tGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGTCAgc                                                      <  1:424534/71‑1 (MQ=255)
                  ccGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtg                                                     >  1:2807830/1‑68 (MQ=255)
                  ccGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtg                                                     >  1:915853/1‑68 (MQ=255)
                   cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgca                                                   <  1:3406803/71‑2 (MQ=255)
                   cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgca                                                   <  1:1148017/71‑2 (MQ=255)
                   cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgc                                                    >  1:3438212/1‑70 (MQ=255)
                    gcacgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg                                                   >  1:2686903/1‑70 (MQ=255)
                       cgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACa                                               >  1:3285942/1‑71 (MQ=255)
                        gcacCGTTAGTCGGCGCAGGCACCACGCGACCGCCAGCAGCGTTctct                                                                     <  1:3950309/48‑1 (MQ=16)
                         cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATg                                             <  1:520067/71‑1 (MQ=255)
                         cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATg                                             <  1:2201729/71‑1 (MQ=255)
                          acCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGt                                            <  1:3827713/71‑1 (MQ=255)
                           ccGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttct                                                                     <  1:4082012/45‑1 (MQ=255)
                              ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                        <  1:2463316/71‑1 (MQ=255)
                              ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                        >  1:1401770/1‑71 (MQ=255)
                               tAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                        >  1:3042194/1‑70 (MQ=255)
                                aGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTa                                                               <  1:2193282/46‑1 (MQ=255)
                                 gTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATcc                                      <  1:2943441/70‑1 (MQ=255)
                                 gTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa                                     <  1:3478794/71‑1 (MQ=255)
                                  tCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGcc                                                         >  1:4110362/1‑50 (MQ=255)
                                  tCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAg                                    <  1:2559309/71‑1 (MQ=255)
                                     gTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                        <  1:2510980/64‑1 (MQ=255)
                                     gTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCg                                 <  1:3659028/71‑1 (MQ=255)
                                      tGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGa                                >  1:1423232/1‑71 (MQ=255)
                                        ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGAt                               <  1:1911099/70‑1 (MQ=255)
                                        ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATg                              <  1:565092/71‑1 (MQ=255)
                                         cGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGt                                            >  1:3777975/1‑56 (MQ=255)
                                          gTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGtt                                           <  1:170599/56‑1 (MQ=255)
                                           ttACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGcc                                                         >  1:3771245/1‑41 (MQ=255)
                                            ctccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACa                           <  1:3673314/68‑1 (MQ=255)
                                             aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa                                     >  1:3858945/1‑59 (MQ=255)
                                             aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa                                     >  1:384980/1‑59 (MQ=255)
                                             aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt                         >  1:3297635/1‑71 (MQ=255)
                                             aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCAAGCGCGTACATGTTGATCCAGTAGATGACAtt                         >  1:3607588/1‑71 (MQ=255)
                                              ccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc                        <  1:52666/71‑1 (MQ=255)
                                               cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTa                                                 <  1:246975/45‑1 (MQ=255)
                                               cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACa                           <  1:3242432/67‑1 (MQ=255)
                                                aCGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg                                                   <  1:1901996/42‑1 (MQ=255)
                                                 cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGt                                                  >  1:1547760/1‑42 (MQ=255)
                                                 cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTg                                          >  1:646775/1‑50 (MQ=255)
                                                  gcgcCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc                        >  1:629693/1‑67 (MQ=255)
                                                   cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACa                                               >  1:2697589/1‑43 (MQ=255)
                                                   cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATc                                       >  1:3685747/1‑51 (MQ=255)
                                                   cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg                   <  1:3147548/71‑1 (MQ=255)
                                                   cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg                   <  1:1480860/71‑1 (MQ=255)
                                                        gccAGCTGCGTTTTCTTCACTAACCGCCCGCGCGTACATGTTGa                                         >  1:2108221/1‑44 (MQ=255)
                                                        gccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGt              <  1:865791/71‑1 (MQ=255)
                                                         ccATCTGCGTTTTCTTCACTAACCGCCAGCTCGTACATGTTGATCCAGTCGAt                               <  1:73069/53‑1 (MQ=38)
                                                             cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc                        <  1:3855222/56‑1 (MQ=255)
                                                              tGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTa            >  1:3576235/1‑67 (MQ=255)
                                                               gCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGtt             >  1:2077162/1‑65 (MQ=255)
                                                               gCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGAt       >  1:1530808/1‑71 (MQ=255)
                                                                 gTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGa                                >  1:2266062/1‑44 (MQ=255)
                                                                  ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGa                             <  1:1608511/46‑1 (MQ=255)
                                                                  ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGa                             >  1:3566833/1‑46 (MQ=255)
                                                                  ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCg                    <  1:1159261/55‑1 (MQ=255)
                                                                  ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGtt    <  1:3172541/71‑1 (MQ=255)
                                                                   tttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGa                             <  1:172813/45‑1 (MQ=255)
                                                                    ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGAt                               >  1:2513199/1‑42 (MQ=255)
                                                                    ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc                        <  1:3831771/49‑1 (MQ=255)
                                                                    ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg                   >  1:1281444/1‑54 (MQ=255)
                                                                    ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTa            >  1:2616262/1‑61 (MQ=255)
                                                                    ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTa   >  1:1043708/1‑70 (MQ=255)
                                                                    ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTAt  >  1:1126300/1‑71 (MQ=255)
                                                                    |                                                                      
AGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTAT  >  minE/2093962‑2094100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑