Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,094,036	A→G	6.7%	S272S (AGT→AGC) ‡	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,094,036	0	A	G	6.7%	209.6 / 6.1	74	S272S (AGT→AGC) ‡	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base A (35/34);  new base G (3/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTG  >  minE/2093966‑2094105                                                                       |                                                                      cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCa                                                                       >  1:2928687/1‑71 (MQ=255) cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACAACGCGCCCGCCAGCTGCGTTTTCTTCa                                                                       >  1:1124931/1‑71 (MQ=255)   gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCAc                                                                      >  1:361529/1‑70 (MQ=255)   gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCAc                                                                      >  1:3214810/1‑70 (MQ=255)    ccGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACt                                                                     >  1:1250371/1‑70 (MQ=255)    ccGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTa                                                                    <  1:139694/71‑1 (MQ=255)     cGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTaa                                                                   <  1:2098593/71‑1 (MQ=255)         ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                                                               >  1:3465786/1‑71 (MQ=255)         ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                                                               >  1:3190474/1‑71 (MQ=255)             tGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAAcc                                                                 >  1:3695737/1‑65 (MQ=255)             tGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGTCAgc                                                           <  1:424534/71‑1 (MQ=255)               ccGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtg                                                          >  1:915853/1‑68 (MQ=255)               ccGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtg                                                          >  1:2807830/1‑68 (MQ=255)                cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgca                                                        <  1:3406803/71‑2 (MQ=255)                cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgca                                                        <  1:1148017/71‑2 (MQ=255)                cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgc                                                         >  1:3438212/1‑70 (MQ=255)                 gcacgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg                                                        >  1:2686903/1‑70 (MQ=255)                    cgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACa                                                    >  1:3285942/1‑71 (MQ=255)                      cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATg                                                  <  1:520067/71‑1 (MQ=255)                      cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATg                                                  <  1:2201729/71‑1 (MQ=255)                       acCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGt                                                 <  1:3827713/71‑1 (MQ=255)                           ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                             <  1:2463316/71‑1 (MQ=255)                           ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                             >  1:1401770/1‑71 (MQ=255)                            tAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                             >  1:3042194/1‑70 (MQ=255)                             aGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTa                                                                    <  1:2193282/46‑1 (MQ=255)                              gTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATcc                                           <  1:2943441/70‑1 (MQ=255)                              gTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa                                          <  1:3478794/71‑1 (MQ=255)                               tCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGcc                                                              >  1:4110362/1‑50 (MQ=255)                               tCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAg                                         <  1:2559309/71‑1 (MQ=255)                                  gTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                             <  1:2510980/64‑1 (MQ=255)                                  gTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCg                                      <  1:3659028/71‑1 (MQ=255)                                   tGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGa                                     >  1:1423232/1‑71 (MQ=255)                                     ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGAt                                    <  1:1911099/70‑1 (MQ=255)                                     ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATg                                   <  1:565092/71‑1 (MQ=255)                                      cGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGt                                                 >  1:3777975/1‑56 (MQ=255)                                       gTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGtt                                                <  1:170599/56‑1 (MQ=255)                                        ttACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGcc                                                              >  1:3771245/1‑41 (MQ=255)                                         ctccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACa                                <  1:3673314/68‑1 (MQ=255)                                          aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa                                          >  1:384980/1‑59 (MQ=255)                                          aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa                                          >  1:3858945/1‑59 (MQ=255)                                          aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt                              >  1:3297635/1‑71 (MQ=255)                                          aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCAAGCGCGTACATGTTGATCCAGTAGATGACAtt                              >  1:3607588/1‑71 (MQ=255)                                           ccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc                             <  1:52666/71‑1 (MQ=255)                                            cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTa                                                      <  1:246975/45‑1 (MQ=255)                                            cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACa                                <  1:3242432/67‑1 (MQ=255)                                             aCGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg                                                        <  1:1901996/42‑1 (MQ=255)                                              cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGt                                                       >  1:1547760/1‑42 (MQ=255)                                              cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTg                                               >  1:646775/1‑50 (MQ=255)                                               gcgcCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc                             >  1:629693/1‑67 (MQ=255)                                                cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACa                                                    >  1:2697589/1‑43 (MQ=255)                                                cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATc                                            >  1:3685747/1‑51 (MQ=255)                                                cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg                        <  1:1480860/71‑1 (MQ=255)                                                cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg                        <  1:3147548/71‑1 (MQ=255)                                                     gccAGCTGCGTTTTCTTCACTAACCGCCCGCGCGTACATGTTGa                                              >  1:2108221/1‑44 (MQ=255)                                                     gccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGt                   <  1:865791/71‑1 (MQ=255)                                                      ccATCTGCGTTTTCTTCACTAACCGCCAGCTCGTACATGTTGATCCAGTCGAt                                    <  1:73069/53‑1 (MQ=38)                                                          cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc                             <  1:3855222/56‑1 (MQ=255)                                                           tGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTa                 >  1:3576235/1‑67 (MQ=255)                                                            gCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGtt                  >  1:2077162/1‑65 (MQ=255)                                                            gCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGAt            >  1:1530808/1‑71 (MQ=255)                                                              gTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGa                                     >  1:2266062/1‑44 (MQ=255)                                                               ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGa                                  >  1:3566833/1‑46 (MQ=255)                                                               ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGa                                  <  1:1608511/46‑1 (MQ=255)                                                               ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCg                         <  1:1159261/55‑1 (MQ=255)                                                               ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGtt         <  1:3172541/71‑1 (MQ=255)                                                                tttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGa                                  <  1:172813/45‑1 (MQ=255)                                                                 ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGAt                                    >  1:2513199/1‑42 (MQ=255)                                                                 ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc                             <  1:3831771/49‑1 (MQ=255)                                                                 ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg                        >  1:1281444/1‑54 (MQ=255)                                                                 ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTa                 >  1:2616262/1‑61 (MQ=255)                                                                 ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTa        >  1:1043708/1‑70 (MQ=255)                                                                 ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTAt       >  1:1126300/1‑71 (MQ=255)                                                                   cttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTaga               <  1:455089/61‑1 (MQ=255)                                                                   cttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATc      <  1:2685446/70‑1 (MQ=255)                                                                     tcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTaga               <  1:2897545/59‑1 (MQ=255)                                                                      cACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTg  >  1:3863971/1‑71 (MQ=255)                                                                       |                                                                      CTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTG  >  minE/2093966‑2094105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑