| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,094,039 | A→C | 7.0% | V271V (GTT→GTG) | tdcG ← | L‑serine dehydratase 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,094,039 | 0 | A | C | 7.0% | 177.3 / 9.8 | 71 | V271V (GTT→GTG) | tdcG | L‑serine dehydratase 3 |
| Reads supporting (aligned to +/- strand): ref base A (32/34); new base C (3/2); total (35/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
CCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGA > minE/2093969‑2094107 | ccGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTa < 1:139694/71‑1 (MQ=255) cGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTaa < 1:2098593/71‑1 (MQ=255) ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc > 1:3465786/1‑71 (MQ=255) ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc > 1:3190474/1‑71 (MQ=255) tGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAAcc > 1:3695737/1‑65 (MQ=255) tGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGTCAgc < 1:424534/71‑1 (MQ=255) ccGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtg > 1:915853/1‑68 (MQ=255) ccGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtg > 1:2807830/1‑68 (MQ=255) cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgca < 1:1148017/71‑2 (MQ=255) cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgca < 1:3406803/71‑2 (MQ=255) cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgc > 1:3438212/1‑70 (MQ=255) gcacgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg > 1:2686903/1‑70 (MQ=255) cgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACa > 1:3285942/1‑71 (MQ=255) cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATg < 1:2201729/71‑1 (MQ=255) cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATg < 1:520067/71‑1 (MQ=255) acCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGt < 1:3827713/71‑1 (MQ=255) ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt < 1:2463316/71‑1 (MQ=255) ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt > 1:1401770/1‑71 (MQ=255) tAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt > 1:3042194/1‑70 (MQ=255) aGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTa < 1:2193282/46‑1 (MQ=255) gTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATcc < 1:2943441/70‑1 (MQ=255) gTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa < 1:3478794/71‑1 (MQ=255) tCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGcc > 1:4110362/1‑50 (MQ=255) tCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAg < 1:2559309/71‑1 (MQ=255) gTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt < 1:2510980/64‑1 (MQ=255) gTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCg < 1:3659028/71‑1 (MQ=255) tGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGa > 1:1423232/1‑71 (MQ=255) ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGAt < 1:1911099/70‑1 (MQ=255) ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATg < 1:565092/71‑1 (MQ=255) cGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGt > 1:3777975/1‑56 (MQ=255) gTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGtt < 1:170599/56‑1 (MQ=255) ttACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGcc > 1:3771245/1‑41 (MQ=255) ctccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACa < 1:3673314/68‑1 (MQ=255) aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa > 1:384980/1‑59 (MQ=255) aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa > 1:3858945/1‑59 (MQ=255) aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt > 1:3297635/1‑71 (MQ=255) aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCAAGCGCGTACATGTTGATCCAGTAGATGACAtt > 1:3607588/1‑71 (MQ=255) ccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc < 1:52666/71‑1 (MQ=255) cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTa < 1:246975/45‑1 (MQ=255) cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACa < 1:3242432/67‑1 (MQ=255) aCGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg < 1:1901996/42‑1 (MQ=255) cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGt > 1:1547760/1‑42 (MQ=255) cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTg > 1:646775/1‑50 (MQ=255) gcgcCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc > 1:629693/1‑67 (MQ=255) cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACa > 1:2697589/1‑43 (MQ=255) cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATc > 1:3685747/1‑51 (MQ=255) cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg < 1:3147548/71‑1 (MQ=255) cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg < 1:1480860/71‑1 (MQ=255) gccAGCTGCGTTTTCTTCACTAACCGCCCGCGCGTACATGTTGa > 1:2108221/1‑44 (MQ=255) gccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGt < 1:865791/71‑1 (MQ=255) ccATCTGCGTTTTCTTCACTAACCGCCAGCTCGTACATGTTGATCCAGTCGAt < 1:73069/53‑1 (MQ=38) cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc < 1:3855222/56‑1 (MQ=255) tGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTa > 1:3576235/1‑67 (MQ=255) gCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGtt > 1:2077162/1‑65 (MQ=255) gCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGAt > 1:1530808/1‑71 (MQ=255) gTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGa > 1:2266062/1‑44 (MQ=255) ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGa > 1:3566833/1‑46 (MQ=255) ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGa < 1:1608511/46‑1 (MQ=255) ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCg < 1:1159261/55‑1 (MQ=255) ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGtt < 1:3172541/71‑1 (MQ=255) tttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGa < 1:172813/45‑1 (MQ=255) ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGAt > 1:2513199/1‑42 (MQ=255) ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc < 1:3831771/49‑1 (MQ=255) ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg > 1:1281444/1‑54 (MQ=255) ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTa > 1:2616262/1‑61 (MQ=255) ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTa > 1:1043708/1‑70 (MQ=255) ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTAt > 1:1126300/1‑71 (MQ=255) cttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTaga < 1:455089/61‑1 (MQ=255) cttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATc < 1:2685446/70‑1 (MQ=255) tcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTaga < 1:2897545/59‑1 (MQ=255) cACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTg > 1:3863971/1‑71 (MQ=255) cTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCg < 1:630737/66‑1 (MQ=255) cTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGa < 1:2038283/71‑1 (MQ=255) tAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCa < 1:439363/42‑1 (MQ=255) | CCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGA > minE/2093969‑2094107 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |