Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,094,042 C→T 6.7% A270A (GCG→GCA)  tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,094,0420CT 6.7% 237.5 / 5.7 74A270A (GCG→GCA) tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base C (35/34);  new base T (3/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

ATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAAC  >  minE/2093974‑2094110
                                                                    |                                                                    
ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                                                                    >  1:3465786/1‑71 (MQ=255)
ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                                                                    >  1:3190474/1‑71 (MQ=255)
    tGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAAcc                                                                      >  1:3695737/1‑65 (MQ=255)
    tGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGTCAgc                                                                <  1:424534/71‑1 (MQ=255)
      ccGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtg                                                               >  1:915853/1‑68 (MQ=255)
      ccGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtg                                                               >  1:2807830/1‑68 (MQ=255)
       cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgca                                                             <  1:1148017/71‑2 (MQ=255)
       cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgca                                                             <  1:3406803/71‑2 (MQ=255)
       cgcacgcaCCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgc                                                              >  1:3438212/1‑70 (MQ=255)
        gcacgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg                                                             >  1:2686903/1‑70 (MQ=255)
           cgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACa                                                         >  1:3285942/1‑71 (MQ=255)
             cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATg                                                       <  1:2201729/71‑1 (MQ=255)
             cacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATg                                                       <  1:520067/71‑1 (MQ=255)
              acCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGt                                                      <  1:3827713/71‑1 (MQ=255)
                  ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                                  >  1:1401770/1‑71 (MQ=255)
                  ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                                  <  1:2463316/71‑1 (MQ=255)
                   tAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                                  >  1:3042194/1‑70 (MQ=255)
                     gTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATcc                                                <  1:2943441/70‑1 (MQ=255)
                     gTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa                                               <  1:3478794/71‑1 (MQ=255)
                      tCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGcc                                                                   >  1:4110362/1‑50 (MQ=255)
                      tCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAg                                              <  1:2559309/71‑1 (MQ=255)
                         gTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                                                  <  1:2510980/64‑1 (MQ=255)
                         gTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCg                                           <  1:3659028/71‑1 (MQ=255)
                          tGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGa                                          >  1:1423232/1‑71 (MQ=255)
                            ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGAt                                         <  1:1911099/70‑1 (MQ=255)
                            ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATg                                        <  1:565092/71‑1 (MQ=255)
                             cGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGt                                                      >  1:3777975/1‑56 (MQ=255)
                              gTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGtt                                                     <  1:170599/56‑1 (MQ=255)
                               ttACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGcc                                                                   >  1:3771245/1‑41 (MQ=255)
                                ctccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACa                                     <  1:3673314/68‑1 (MQ=255)
                                 aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa                                               >  1:3858945/1‑59 (MQ=255)
                                 aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCa                                               >  1:384980/1‑59 (MQ=255)
                                 aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt                                   >  1:3297635/1‑71 (MQ=255)
                                 aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCAAGCGCGTACATGTTGATCCAGTAGATGACAtt                                   >  1:3607588/1‑71 (MQ=255)
                                  ccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc                                  <  1:52666/71‑1 (MQ=255)
                                   cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTa                                                           <  1:246975/45‑1 (MQ=255)
                                   cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACa                                     <  1:3242432/67‑1 (MQ=255)
                                    aCGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg                                                             <  1:1901996/42‑1 (MQ=255)
                                     cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGt                                                            >  1:1547760/1‑42 (MQ=255)
                                     cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTg                                                    >  1:646775/1‑50 (MQ=255)
                                      gcgcCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc                                  >  1:629693/1‑67 (MQ=255)
                                       cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACa                                                         >  1:2697589/1‑43 (MQ=255)
                                       cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATc                                                 >  1:3685747/1‑51 (MQ=255)
                                       cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg                             <  1:1480860/71‑1 (MQ=255)
                                       cgcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg                             <  1:3147548/71‑1 (MQ=255)
                                            gccAGCTGCGTTTTCTTCACTAACCGCCCGCGCGTACATGTTGa                                                   >  1:2108221/1‑44 (MQ=255)
                                            gccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGt                        <  1:865791/71‑1 (MQ=255)
                                             ccATCTGCGTTTTCTTCACTAACCGCCAGCTCGTACATGTTGATCCAGTCGAt                                         <  1:73069/53‑1 (MQ=38)
                                                 cTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc                                  <  1:3855222/56‑1 (MQ=255)
                                                  tGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTa                      >  1:3576235/1‑67 (MQ=255)
                                                   gCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGtt                       >  1:2077162/1‑65 (MQ=255)
                                                   gCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGAt                 >  1:1530808/1‑71 (MQ=255)
                                                     gTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGa                                          >  1:2266062/1‑44 (MQ=255)
                                                      ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGa                                       >  1:3566833/1‑46 (MQ=255)
                                                      ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGa                                       <  1:1608511/46‑1 (MQ=255)
                                                      ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCg                              <  1:1159261/55‑1 (MQ=255)
                                                      ttttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGtt              <  1:3172541/71‑1 (MQ=255)
                                                       tttCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGa                                       <  1:172813/45‑1 (MQ=255)
                                                        ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGAt                                         >  1:2513199/1‑42 (MQ=255)
                                                        ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc                                  <  1:3831771/49‑1 (MQ=255)
                                                        ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCgg                             >  1:1281444/1‑54 (MQ=255)
                                                        ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTa                      >  1:2616262/1‑61 (MQ=255)
                                                        ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTa             >  1:1043708/1‑70 (MQ=255)
                                                        ttcttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTAt            >  1:1126300/1‑71 (MQ=255)
                                                          cttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTaga                    <  1:455089/61‑1 (MQ=255)
                                                          cttcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATc           <  1:2685446/70‑1 (MQ=255)
                                                            tcACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTaga                    <  1:2897545/59‑1 (MQ=255)
                                                             cACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTg       >  1:3863971/1‑71 (MQ=255)
                                                               cTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCg          <  1:630737/66‑1 (MQ=255)
                                                               cTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGa     <  1:2038283/71‑1 (MQ=255)
                                                                tAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCa                                 <  1:439363/42‑1 (MQ=255)
                                                                  aCCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTc                                  >  1:845725/1‑39 (MQ=255)
                                                                  aCCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAAc  >  1:3637733/1‑71 (MQ=255)
                                                                  aCCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAAc  >  1:316827/1‑71 (MQ=255)
                                                                  aCCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAAc  >  1:948987/1‑71 (MQ=255)
                                                                    |                                                                    
ATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGATCGTTAGAGATGTTATCGCTGGAAAC  >  minE/2093974‑2094110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑