| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,130,622 | T→C | 7.9% | I95V (ATT→GTT) | mtr ← | tryptophan transporter of high affinity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,130,622 | 0 | T | C | 7.9% | 287.9 / 13.6 | 103 | I95V (ATT→GTT) | mtr | tryptophan transporter of high affinity |
| Reads supporting (aligned to +/- strand): ref base T (48/46); new base C (5/3); total (54/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
AAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACC > minE/2130552‑2130691 | aaGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAAt > 1:2323172/1‑71 (MQ=255) gtgtGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa < 1:2686479/71‑1 (MQ=255) gtgtGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa < 1:3865585/71‑1 (MQ=255) tgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAAt > 1:2347006/1‑71 (MQ=255) aTGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATg < 1:1924994/70‑1 (MQ=255) aTGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc < 1:2317618/71‑1 (MQ=255) aTGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc < 1:2784812/71‑1 (MQ=255) aTGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc < 1:2941522/71‑1 (MQ=255) tGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa < 1:3739414/65‑1 (MQ=255) tGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGcc > 1:2256645/1‑71 (MQ=255) gCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGAAAATGCCg > 1:3302640/1‑71 (MQ=255) cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaaa > 1:2063687/1‑65 (MQ=255) cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGt > 1:1055217/1‑71 (MQ=255) aaTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa < 1:687450/60‑1 (MQ=255) aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc > 1:1990612/1‑71 (MQ=255) tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc > 1:3694213/1‑70 (MQ=255) tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc > 1:467450/1‑70 (MQ=255) aCCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaaa > 1:416123/1‑56 (MQ=255) cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTc < 1:3558437/71‑1 (MQ=255) cTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaaa > 1:1394203/1‑52 (MQ=255) tGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAg > 1:3197796/1‑71 (MQ=255) ggCAGAAATATAGGCATAGGTCTGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc > 1:1087151/1‑71 (MQ=255) ggCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc > 1:4028968/1‑71 (MQ=255) gCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAAta < 1:364309/51‑2 (MQ=255) gCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAAta < 1:447295/51‑2 (MQ=255) gCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGcc < 1:1042540/71‑1 (MQ=255) cAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt > 1:792759/1‑71 (MQ=255) gAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa < 1:3672882/44‑1 (MQ=255) gAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCttt > 1:3141263/1‑71 (MQ=255) aaTATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaaa > 1:3790496/1‑44 (MQ=255) aaTATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGa > 1:808820/1‑53 (MQ=255) atatAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc > 1:2056406/1‑71 (MQ=255) atatAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc > 1:1979609/1‑71 (MQ=255) ataGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc > 1:853856/1‑69 (MQ=255) taGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAg > 1:60549/1‑71 (MQ=255) ggCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCa < 1:3181651/71‑1 (MQ=255) ggCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCa < 1:3079695/71‑1 (MQ=255) gCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaa < 1:2764728/71‑1 (MQ=255) aTAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt < 1:279030/71‑1 (MQ=255) tAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt < 1:2176344/41‑1 (MQ=255) aGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt > 1:1169701/1‑71 (MQ=255) ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt > 1:2929401/1‑39 (MQ=255) ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt > 1:891648/1‑71 (MQ=255) ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt < 1:648310/71‑1 (MQ=255) ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt < 1:1563828/70‑1 (MQ=255) cgTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt < 1:294514/69‑1 (MQ=255) gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt < 1:2490616/46‑1 (MQ=255) gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc < 1:2175486/68‑1 (MQ=255) cAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt > 1:725540/1‑68 (MQ=255) cAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt > 1:806503/1‑68 (MQ=255) cAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt > 1:3021278/1‑67 (MQ=255) cAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg > 1:3704886/1‑70 (MQ=255) aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaa < 1:1158925/62‑1 (MQ=255) aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaa < 1:786837/62‑1 (MQ=255) aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTgg > 1:23075/1‑70 (MQ=255) aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt < 1:3302764/71‑1 (MQ=255) gATATAGAGCACAAAGGCAATGGAAATGCCGTTGa > 1:630764/1‑35 (MQ=255) gATATAGAGCACAAAGGCAATGGAAATGCCGTTGa > 1:2027902/1‑35 (MQ=255) gATATAGAGCACAAAGGCAATGGAAATGCCGTTGa > 1:2707977/1‑35 (MQ=255) gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt < 1:766356/66‑1 (MQ=255) gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa > 1:54848/1‑71 (MQ=255) gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa > 1:87463/1‑71 (MQ=255) gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa > 1:722123/1‑71 (MQ=255) gATATAGAGCACAAAGGCAAAGGAAAAGCCGTTGa > 1:1059276/1‑35 (MQ=38) atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTcc > 1:3754838/1‑43 (MQ=255) atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt < 1:668450/71‑1 (MQ=255) tataGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATg < 1:2425851/71‑1 (MQ=255) ataGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt < 1:10273/63‑1 (MQ=255) ataGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt < 1:3277321/69‑1 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt > 1:1856167/1‑62 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt < 1:3452425/65‑1 (MQ=255) taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt < 1:1172475/71‑1 (MQ=255) taGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGgt > 1:3116489/1‑71 (MQ=255) agagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt > 1:851544/1‑60 (MQ=255) agagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt > 1:2924587/1‑60 (MQ=255) gagaACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGgtgt > 1:1613642/1‑71 (MQ=255) gagaACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGgtgt > 1:2093317/1‑71 (MQ=255) gagaACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGgtgt > 1:735645/1‑71 (MQ=255) gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa > 1:522400/1‑65 (MQ=255) gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgtgt > 1:486696/1‑71 (MQ=255) gCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTc > 1:4140395/1‑70 (MQ=255) gCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTc > 1:3842845/1‑70 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt < 1:929188/63‑1 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:366383/71‑1 (MQ=255) cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:347323/71‑1 (MQ=255) acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa < 1:3386665/70‑1 (MQ=255) caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATgg > 1:3137214/1‑63 (MQ=255) aaaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAAc < 1:672696/71‑1 (MQ=255) aaaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAAc < 1:1380269/71‑1 (MQ=255) aaGGCAATGGAAATGCCATTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACt > 1:3053254/1‑71 (MQ=255) aaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTAAAACt > 1:2405443/1‑71 (MQ=255) aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc > 1:3189852/1‑71 (MQ=255) ggCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt < 1:301251/49‑1 (MQ=255) gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGc > 1:1345081/1‑42 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt < 1:365695/50‑1 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa < 1:1739325/52‑1 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc < 1:1911504/71‑1 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc < 1:3574551/71‑1 (MQ=255) aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc < 1:1246246/71‑1 (MQ=255) tGGAAATGACGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAAc < 1:2877459/63‑1 (MQ=255) | AAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACC > minE/2130552‑2130691 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |