Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,130,625 A→T 7.4% S94T (TCC→ACC)  mtr ← tryptophan transporter of high affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,130,6250AT 7.4% 309.6 / 14.2 108S94T (TCC→ACC) mtrtryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base A (56/44);  new base T (5/3);  total (61/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 117 total aligned reads displayed.

GTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGA  >  minE/2130555‑2130693
                                                                      |                                                                    
gtgtGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                                                                      <  1:3865585/71‑1 (MQ=255)
gtgtGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                                                                      <  1:2686479/71‑1 (MQ=255)
   tgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAAt                                                                   >  1:2347006/1‑71 (MQ=255)
     aTGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATg                                                                  <  1:1924994/70‑1 (MQ=255)
     aTGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc                                                                 <  1:2941522/71‑1 (MQ=255)
     aTGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc                                                                 <  1:2784812/71‑1 (MQ=255)
      tGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                                                                      <  1:3739414/65‑1 (MQ=255)
      tGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGcc                                                                >  1:2256645/1‑71 (MQ=255)
       gCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGAAAATGCCg                                                               >  1:3302640/1‑71 (MQ=255)
        cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaaa                                                                    >  1:2063687/1‑65 (MQ=255)
        cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGt                                                              >  1:1055217/1‑71 (MQ=255)
           aaTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                                                                      <  1:687450/60‑1 (MQ=255)
            aTCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc                                                          >  1:1990612/1‑71 (MQ=255)
             tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAcc                                                         <  1:546231/71‑1 (MQ=255)
             tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc                                                          >  1:3694213/1‑70 (MQ=255)
             tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc                                                          >  1:467450/1‑70 (MQ=255)
                   cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTc                                                   <  1:3558437/71‑1 (MQ=255)
                     cTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaaa                                                                    >  1:1394203/1‑52 (MQ=255)
                      tGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAg                                                >  1:3197796/1‑71 (MQ=255)
                       ggCAGAAATATAGGCATAGGTCTGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc                                               >  1:1087151/1‑71 (MQ=255)
                       ggCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc                                               >  1:4028968/1‑71 (MQ=255)
                        gCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAAta                                                                  <  1:364309/51‑2 (MQ=255)
                        gCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAAta                                                                  <  1:447295/51‑2 (MQ=255)
                        gCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGcc                                              <  1:1042540/71‑1 (MQ=255)
                         cAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCt                                             >  1:792759/1‑71 (MQ=255)
                           gAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCttt                                           >  1:3141263/1‑71 (MQ=255)
                             aaTATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaaa                                                                    >  1:3790496/1‑44 (MQ=255)
                             aaTATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGa                                                           >  1:808820/1‑53 (MQ=255)
                              atatAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc                                        >  1:2056406/1‑71 (MQ=255)
                              atatAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc                                        >  1:1979609/1‑71 (MQ=255)
                                ataGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc                                        >  1:853856/1‑69 (MQ=255)
                                   ggCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCa                                   <  1:3181651/71‑1 (MQ=255)
                                   ggCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCa                                   <  1:3079695/71‑1 (MQ=255)
                                    gCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaa                                  <  1:2764728/71‑1 (MQ=255)
                                      aTAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt                                <  1:279030/71‑1 (MQ=255)
                                       tAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                                                             <  1:2176344/41‑1 (MQ=255)
                                        aGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                              >  1:1169701/1‑71 (MQ=255)
                                         ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                                                             >  1:2929401/1‑39 (MQ=255)
                                         ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt                             >  1:891648/1‑71 (MQ=255)
                                         ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt                             <  1:648310/71‑1 (MQ=255)
                                         ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                              <  1:1993044/70‑1 (MQ=255)
                                         cgTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                              <  1:294514/69‑1 (MQ=255)
                                          gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt                                                     <  1:2490616/46‑1 (MQ=255)
                                          gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc                               <  1:2175486/68‑1 (MQ=255)
                                            cAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt                             >  1:806503/1‑68 (MQ=255)
                                            cAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                              >  1:3021278/1‑67 (MQ=255)
                                            cAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg                           >  1:3704886/1‑70 (MQ=255)
                                             aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaa                                  <  1:786837/62‑1 (MQ=255)
                                             aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTgg                          >  1:23075/1‑70 (MQ=255)
                                             aGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt                         <  1:3302764/71‑1 (MQ=255)
                                               gATATAGAGCACAAAGGCAATGGAAATGCCGTTGa                                                           >  1:2027902/1‑35 (MQ=255)
                                               gATATAGAGCACAAAGGCAATGGAAATGCCGTTGa                                                           >  1:630764/1‑35 (MQ=255)
                                               gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                            <  1:766356/66‑1 (MQ=255)
                                               gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                       >  1:87463/1‑71 (MQ=255)
                                               gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                       >  1:54848/1‑71 (MQ=255)
                                               gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                       >  1:722123/1‑71 (MQ=255)
                                               gATATAGAGCACAAAGGCAAAGGAAAAGCCGTTGa                                                           >  1:1059276/1‑35 (MQ=38)
                                                atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTcc                                                  >  1:3754838/1‑43 (MQ=255)
                                                atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt                      <  1:668450/71‑1 (MQ=255)
                                                 tataGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATg                     <  1:2425851/71‑1 (MQ=255)
                                                  ataGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                            <  1:10273/63‑1 (MQ=255)
                                                  ataGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt                      <  1:3277321/69‑1 (MQ=255)
                                                   taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                            >  1:1856167/1‑62 (MQ=255)
                                                   taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt                         <  1:3452425/65‑1 (MQ=255)
                                                   taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgt                   <  1:1172475/71‑1 (MQ=255)
                                                   taGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGgt                   >  1:3116489/1‑71 (MQ=255)
                                                    agagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt                             >  1:851544/1‑60 (MQ=255)
                                                     gagaACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGgtgt                 >  1:1613642/1‑71 (MQ=255)
                                                     gagaACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGgtgt                 >  1:2093317/1‑71 (MQ=255)
                                                     gagaACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGgtgt                 >  1:735645/1‑71 (MQ=255)
                                                     gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                       >  1:522400/1‑65 (MQ=255)
                                                     gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgtgt                 >  1:486696/1‑71 (MQ=255)
                                                     gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgtgt                 >  1:2892877/1‑71 (MQ=255)
                                                       gCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTc                >  1:4140395/1‑70 (MQ=255)
                                                        cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt                      <  1:929188/63‑1 (MQ=255)
                                                        cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa              <  1:366383/71‑1 (MQ=255)
                                                        cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa              <  1:3484519/71‑1 (MQ=255)
                                                         acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa              <  1:3386665/70‑1 (MQ=255)
                                                          caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATgg                    >  1:3137214/1‑63 (MQ=255)
                                                           aaaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAAc           <  1:672696/71‑1 (MQ=255)
                                                           aaaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAAc           <  1:1380269/71‑1 (MQ=255)
                                                            aaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTAAAACt          >  1:2405443/1‑71 (MQ=255)
                                                              ggCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                              <  1:301251/49‑1 (MQ=255)
                                                               gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGc                                    >  1:1345081/1‑42 (MQ=255)
                                                                  aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt                         <  1:365695/50‑1 (MQ=255)
                                                                  aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                       <  1:1739325/52‑1 (MQ=255)
                                                                  aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc    <  1:3574551/71‑1 (MQ=255)
                                                                  aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc    <  1:1246246/71‑1 (MQ=255)
                                                                  aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc    <  1:1911504/71‑1 (MQ=255)
                                                                   tGGAAATGACGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAAc           <  1:2877459/63‑1 (MQ=255)
                                                                    ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTgg                          >  1:3712199/1‑47 (MQ=255)
                                                                    ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg                           >  1:109224/1‑46 (MQ=255)
                                                                    ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg                           >  1:3994773/1‑46 (MQ=255)
                                                                    ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa            >  1:265509/1‑61 (MQ=255)
                                                                    ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa              <  1:3918977/59‑1 (MQ=255)
                                                                    ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGa  >  1:3046795/1‑71 (MQ=255)
                                                                    ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGa  >  1:4134694/1‑71 (MQ=255)
                                                                    ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGAAAATCTTTg                           >  1:1864507/1‑46 (MQ=255)
                                                                     gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg                           >  1:2277203/1‑45 (MQ=255)
                                                                     gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATg                     >  1:2759149/1‑51 (MQ=255)
                                                                      |                                                                    
GTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGA  >  minE/2130555‑2130693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: