| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,140,377 | T→A | 11.1% | D244V (GAT→GTT) | infB ← | fused protein chain initiation factor 2, IF2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,140,377 | 0 | T | A | 11.1% | 238.7 / 20.0 | 90 | D244V (GAT→GTT) | infB | fused protein chain initiation factor 2, IF2 |
| Reads supporting (aligned to +/- strand): ref base T (31/49); new base A (5/5); total (36/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
TTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGG > minE/2140307‑2140447 | tttgttGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAt > 1:230167/1‑71 (MQ=255) tgttgCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATc > 1:1704543/1‑70 (MQ=255) ttgCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCt < 1:1372049/71‑1 (MQ=255) tgCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGc > 1:460325/1‑69 (MQ=255) ccTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTc < 1:2070082/71‑1 (MQ=255) cTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCttt > 1:3677323/1‑69 (MQ=255) cTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCg > 1:3842567/1‑71 (MQ=255) ttcttcGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGtc > 1:160476/1‑71 (MQ=255) tcttcGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAt > 1:3146660/1‑60 (MQ=255) tcttcGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGtct > 1:1090174/1‑71 (MQ=255) tcttcGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGtc < 1:276013/70‑1 (MQ=255) ttcGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGtcttc > 1:493716/1‑71 (MQ=255) cGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGtctt < 1:788259/68‑1 (MQ=255) cGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGtcttct < 1:2489949/70‑1 (MQ=255) gACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGcc < 1:1205661/71‑1 (MQ=255) cgcgCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTg < 1:415165/71‑1 (MQ=255) cgcgCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTg < 1:2686128/71‑1 (MQ=255) gcgcTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCTCTTTCGTCTTCTGCCTg < 1:1214311/70‑1 (MQ=255) gcgcTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTg < 1:2447316/70‑1 (MQ=255) gcgcTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTgg > 1:3897163/1‑71 (MQ=255) cgcTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTc < 1:322260/57‑1 (MQ=255) gctTTCGCGTCACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCtt < 1:2624612/51‑1 (MQ=255) ctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAg < 1:2837696/69‑1 (MQ=255) ctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGc < 1:2954257/70‑1 (MQ=255) ctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCa > 1:1538349/1‑71 (MQ=255) ctTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCa > 1:3188144/1‑71 (MQ=255) ttCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCAt > 1:3554874/1‑70 (MQ=255) tCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGc > 1:2017923/1‑63 (MQ=255) tCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATg < 1:422260/70‑1 (MQ=255) gTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCt < 1:1819560/42‑1 (MQ=255) gTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCt < 1:2761721/42‑1 (MQ=255) gTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAg < 1:3187810/62‑1 (MQ=255) gTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:1464170/71‑1 (MQ=255) gTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:718016/71‑1 (MQ=255) gTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCAAGt > 1:1928051/1‑67 (MQ=255) ttACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCa > 1:997239/1‑63 (MQ=255) ttACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:2367835/70‑1 (MQ=255) tACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGt > 1:915950/1‑45 (MQ=255) tACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGTCTGGCGAGCATGTTgag > 1:652772/1‑69 (MQ=255) tACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag > 1:1768729/1‑69 (MQ=255) aCGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGtt > 1:485012/1‑65 (MQ=255) aCGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAag > 1:2330502/1‑71 (MQ=255) aCGACCACGGCCACGGCCGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAag > 1:2703072/1‑71 (MQ=255) cGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAagt < 1:2681875/71‑1 (MQ=255) cGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAag < 1:3485994/70‑1 (MQ=255) aCCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTg < 1:451639/47‑1 (MQ=255) cacggccacggcCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGcc > 1:3968191/1‑47 (MQ=255) cacggccacggcCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTg < 1:1904078/71‑1 (MQ=255) cacggccacggcCGCCTTCGACTTCACGAACGCTTTCGTCTTCCGCCTGGCGAGCATGTTGAGAagtagt > 1:1472123/1‑70 (MQ=255) ggccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACg < 1:3653677/71‑1 (MQ=255) gccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGt < 1:2552257/71‑1 (MQ=255) gccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGt < 1:4139089/71‑1 (MQ=255) gccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGt < 1:1141604/71‑1 (MQ=255) gccacggccGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGt < 1:2132710/71‑1 (MQ=255) ccacggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGt < 1:916714/70‑1 (MQ=255) acggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGa > 1:2782174/1‑70 (MQ=255) cggccGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATa < 1:2262967/71‑1 (MQ=255) cggccGCCTTCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATa < 1:1620568/71‑1 (MQ=255) ccgccTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAagta > 1:1418210/1‑57 (MQ=255) cgccTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:2220014/51‑1 (MQ=255) gccTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCa < 1:2198922/43‑1 (MQ=255) ttCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag > 1:2845967/1‑47 (MQ=255) ttCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTg < 1:3160695/60‑1 (MQ=255) ttCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTg < 1:2424450/60‑1 (MQ=255) ttCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTg < 1:199974/60‑1 (MQ=255) ttCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTg < 1:2774448/60‑1 (MQ=255) ttCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCg < 1:4018164/67‑1 (MQ=255) ttCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAag < 1:2271277/50‑1 (MQ=255) ttCGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAag < 1:2276058/50‑1 (MQ=255) tCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTgg > 1:3264173/1‑70 (MQ=255) tCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTgg > 1:3888524/1‑70 (MQ=255) cGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGa > 1:928205/1‑54 (MQ=255) cGACTTCACGAACGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGaa > 1:3749343/1‑71 (MQ=255) agACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGa > 1:2959490/2‑70 (MQ=255) aCTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTgg < 1:851556/67‑1 (MQ=255) cTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCt < 1:1757000/71‑1 (MQ=255) cACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:1343898/39‑1 (MQ=255) cACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAAt > 1:424384/1‑66 (MQ=255) cACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCtt < 1:4091066/69‑1 (MQ=255) cACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCtt < 1:846296/69‑1 (MQ=255) cACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCtt > 1:1601054/1‑69 (MQ=255) cACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCtt > 1:35549/1‑69 (MQ=255) aCGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:1718053/38‑1 (MQ=255) aCGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAAt > 1:3543186/1‑56 (MQ=255) aCGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAAt > 1:2884161/1‑56 (MQ=255) aCGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCt < 1:854779/67‑1 (MQ=255) aCGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAg > 1:3659386/1‑71 (MQ=255) aTCGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:3662326/35‑1 (MQ=255) aTCGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:769128/35‑1 (MQ=255) aTCGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:3663259/35‑1 (MQ=255) aTCGCTTTCGTCTTCTGCCTGGCGAGCATGTTgag < 1:2568588/35‑1 (MQ=255) aTCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTg < 1:126231/43‑1 (MQ=255) tCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTc > 1:1095714/1‑69 (MQ=255) tCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCgg > 1:2442217/1‑71 (MQ=255) | TTTGTTGCCTTTCTTCGGACGCGCTGCTTTCGCGTTACGACCACGGCCACGGCCGCCTTCGACTTCACGATCGCTTTCGTCTTCTGCCTGGCGAGCATGTTGAGAAGTAGTGACGTGATAATCGCTGGAATCTTCAGTCGG > minE/2140307‑2140447 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |