| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,155,567 | A→T | 7.5% | intergenic (+78/+108) | dacB → / ← obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,155,567 | 0 | A | T | 7.5% | 144.0 / 4.4 | 53 | intergenic (+78/+108) | dacB/obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Reads supporting (aligned to +/- strand): ref base A (24/25); new base T (2/2); total (26/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGC > minE/2155504‑2155636 | cGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:4033543/64‑1 (MQ=255) ggCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCtt < 1:710142/71‑1 (MQ=255) cacaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa > 1:673511/1‑63 (MQ=255) cacaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTAt < 1:1407639/71‑1 (MQ=255) caTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATcc < 1:2461240/71‑1 (MQ=255) aTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCg < 1:169438/71‑1 (MQ=255) tGGCCGGGGCTTAAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCgg > 1:1481361/1‑71 (MQ=255) ggggCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa > 1:1901834/1‑70 (MQ=255) aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGt > 1:3904086/1‑71 (MQ=255) aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGt < 1:1843593/71‑1 (MQ=255) attgttGACAAGGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg > 1:3074179/1‑70 (MQ=11) tattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa > 1:1460514/1‑42 (MQ=255) tattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa > 1:138373/1‑42 (MQ=255) ttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAAt < 1:3978830/71‑1 (MQ=255) gACAAATTGCGCTTTATGT‑TGCCGGATGCGGCGTa > 1:1374726/1‑35 (MQ=0) gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt > 1:1963225/1‑70 (MQ=40) aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc < 1:2485088/70‑1 (MQ=255) aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc < 1:411988/70‑1 (MQ=39) aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc < 1:300113/70‑1 (MQ=39) cAAATTGCGCTTTATGT‑TGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCaa > 1:242103/1‑70 (MQ=11) cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa < 1:625065/71‑1 (MQ=25) cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa > 1:2413976/1‑71 (MQ=35) acaGTGCGCTTTGTTTATGCCAGATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCaa > 1:3598848/3‑70 (MQ=11) aaGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa > 1:85039/1‑71 (MQ=38) aGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa > 1:1464419/1‑70 (MQ=39) gcTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc < 1:1571595/71‑1 (MQ=39) cTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc > 1:187516/1‑70 (MQ=40) ttGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATAtt > 1:3317833/1‑66 (MQ=40) tGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAAtt > 1:294861/1‑71 (MQ=255) gTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc > 1:2543323/1‑71 (MQ=255) gTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc > 1:580429/1‑71 (MQ=255) tttATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCaata < 1:3599264/59‑3 (MQ=255) tATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatat > 1:1554861/1‑60 (MQ=255) tATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc‑tc < 1:2417866/70‑1 (MQ=255) aTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTc‑tct < 1:109204/70‑1 (MQ=255) gCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg > 1:2310589/1‑70 (MQ=255) ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg > 1:3414943/1‑69 (MQ=255) cGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc < 1:1244988/63‑1 (MQ=255) ggATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAgg > 1:1199472/1‑71 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACata > 1:1385463/1‑52 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg < 1:2005899/66‑1 (MQ=255) aTGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCtt < 1:2525949/64‑1 (MQ=255) aTGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCtt < 1:2520956/64‑1 (MQ=255) aTGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCtt < 1:2042141/64‑1 (MQ=255) aTGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg < 1:3420709/65‑1 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc < 1:2928581/44‑1 (MQ=38) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCt > 1:286787/1‑71 (MQ=255) cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCaaa > 1:3315927/1‑39 (MQ=255) cggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGa > 1:53033/1‑71 (MQ=255) cggcgTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCt < 1:1238079/70‑1 (MQ=11) ggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa < 1:858955/45‑1 (MQ=255) ggcgTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGa > 1:2890438/1‑71 (MQ=14) gcgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGAt > 1:924625/1‑70 (MQ=255) cgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGAt > 1:4094102/1‑69 (MQ=255) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa < 1:1949693/41‑1 (MQ=255) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa < 1:3514097/41‑1 (MQ=255) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa < 1:3162022/41‑1 (MQ=255) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGc < 1:2910537/71‑1 (MQ=255) | CGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGC > minE/2155504‑2155636 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |