| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,155,591 | T→A | 5.4% | intergenic (+102/+84) | dacB → / ← obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,155,591 | 0 | T | A | 5.4% | 272.8 / 6.1 | 96 | intergenic (+102/+84) | dacB/obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Reads supporting (aligned to +/- strand): ref base T (51/39); new base A (3/2); total (54/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
AGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGT > minE/2155524‑2155661 | aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGt < 1:1843593/71‑1 (MQ=255)aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGt > 1:3904086/1‑71 (MQ=255) attgttGACAAGGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg > 1:3074179/1‑70 (MQ=11) ttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAAt < 1:3978830/71‑1 (MQ=255) gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAAtt > 1:1963225/1‑70 (MQ=40) aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc < 1:2485088/70‑1 (MQ=255) aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc < 1:300113/70‑1 (MQ=39) aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc < 1:411988/70‑1 (MQ=39) cAAATTGCGCTTTATGT‑TGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCaa > 1:242103/1‑70 (MQ=11) cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa < 1:625065/71‑1 (MQ=25) cAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa > 1:2413976/1‑71 (MQ=35) acaGTGCGCTTTGTTTATGCCAGATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTTCCAAATTCaa > 1:3598848/3‑70 (MQ=11) aaGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa > 1:85039/1‑71 (MQ=38) aGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa > 1:1464419/1‑70 (MQ=39) gcTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc < 1:1571595/71‑1 (MQ=39) cTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc > 1:187516/1‑70 (MQ=40) ttGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATAtt > 1:3317833/1‑66 (MQ=40) tGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAAtt > 1:294861/1‑71 (MQ=255) gTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc > 1:580429/1‑71 (MQ=255) gTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc > 1:2543323/1‑71 (MQ=255) tttATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAGCGTGCAAATTCaata < 1:3599264/59‑3 (MQ=255) tATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatat > 1:1554861/1‑60 (MQ=255) tATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc‑tc < 1:2417866/70‑1 (MQ=255) aTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTc‑tct < 1:109204/70‑1 (MQ=255) gCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg > 1:2310589/1‑70 (MQ=255) ccGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg > 1:3414943/1‑69 (MQ=255) cGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc < 1:1244988/63‑1 (MQ=255) ggATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAgg > 1:1199472/1‑71 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACata > 1:1385463/1‑52 (MQ=255) gATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg < 1:2005899/66‑1 (MQ=255) aTGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCtt < 1:2520956/64‑1 (MQ=255) aTGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCtt < 1:2042141/64‑1 (MQ=255) aTGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCtt < 1:2525949/64‑1 (MQ=255) aTGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg < 1:3420709/65‑1 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc < 1:2928581/44‑1 (MQ=38) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCt > 1:286787/1‑71 (MQ=255) cggcgTTAACGCCTTATCCGGCCTACAAAATCGTGCaaa > 1:3315927/1‑39 (MQ=255) cggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGa > 1:53033/1‑71 (MQ=255) cggcgTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCt < 1:1238079/70‑1 (MQ=11) ggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa < 1:858955/45‑1 (MQ=255) ggcgTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGa > 1:2890438/1‑71 (MQ=14) gcgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGAt > 1:924625/1‑70 (MQ=255) cgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGAt > 1:4094102/1‑69 (MQ=255) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa < 1:3162022/41‑1 (MQ=255) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa < 1:3514097/41‑1 (MQ=255) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa < 1:1949693/41‑1 (MQ=255) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGc < 1:2910537/71‑1 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACata > 1:1117306/1‑41 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTc‑tct > 1:3547021/1‑53 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTa > 1:221876/1‑57 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAgga < 1:973272/60‑2 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCg > 1:380681/1‑70 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCg > 1:1533958/1‑70 (MQ=255) aaCGCCTTATCCGGCCTACAAAATCCTGCAAATTCAACATATTGCa > 1:3715942/1‑46 (MQ=255) aaCGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCg > 1:3957879/1‑71 (MQ=11) cGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACata > 1:383228/1‑39 (MQ=40) cGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGc < 1:1573077/43‑1 (MQ=38) cGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg > 1:3306831/1‑53 (MQ=255) cTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTa > 1:3476454/1‑52 (MQ=255) ttATCCGGCCTACATAATCGTGCAAATTCAACATATTGCAATtc‑tc < 1:1403654/46‑1 (MQ=255) tATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:1262877/1‑71 (MQ=255) aTCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCtt > 1:1560208/1‑46 (MQ=255) aTCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCtt > 1:110641/1‑46 (MQ=255) aTCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCtt > 1:2851613/1‑46 (MQ=255) aTCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGAt > 1:2462756/1‑57 (MQ=255) aTCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:12827/1‑70 (MQ=255) aTCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:192915/1‑70 (MQ=255) aTCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:2442764/1‑70 (MQ=255) tCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc‑tc < 1:765947/43‑1 (MQ=255) tCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCa < 1:1051956/71‑1 (MQ=255) ccGGCCTACAAAATCGTGCAAATTCAACATATTGCAATtc‑tc < 1:1874577/42‑1 (MQ=255) cGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAg > 1:3777135/1‑70 (MQ=255) cGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAg > 1:3772497/1‑70 (MQ=255) ggCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCtt > 1:2745337/1‑42 (MQ=255) ggCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGc > 1:302556/1‑71 (MQ=255) tACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAgcg < 1:519756/59‑1 (MQ=255) tACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAgcg < 1:1384782/59‑1 (MQ=255) tACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAgcg < 1:1273044/59‑1 (MQ=255) tACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCa < 1:1218725/61‑1 (MQ=255) tACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCa < 1:1223280/61‑1 (MQ=255) tACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAgc < 1:2559198/59‑1 (MQ=2) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTg < 1:2486175/37‑1 (MQ=255) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGt < 1:1136094/53‑1 (MQ=255) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAgcgc < 1:632625/58‑1 (MQ=255) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:1116796/1‑60 (MQ=255) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCAt > 1:3278021/1‑60 (MQ=255) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCa < 1:4085790/62‑1 (MQ=255) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCa < 1:2175808/62‑1 (MQ=255) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCa < 1:3014952/62‑1 (MQ=255) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAttt > 1:1587914/1‑71 (MQ=255) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAttt > 1:429785/1‑71 (MQ=255) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAttt > 1:91834/1‑71 (MQ=255) cAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAttt > 1:1026083/1‑71 (MQ=255) aaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGc > 1:3694941/1‑49 (MQ=255) aaaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTgg > 1:1141643/1‑71 (MQ=255) aaTCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGAttt < 1:3397377/68‑1 (MQ=255) tCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAgc > 1:3944330/1‑51 (MQ=255) tCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGt < 1:1613918/71‑1 (MQ=255) tCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGt < 1:2526554/71‑1 (MQ=255) tCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGt < 1:3271417/71‑1 (MQ=255) | AGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTC‑TCTTGTAGGCCTGATAAGCGTAGCGCATCAGGCTGATTTGGCGT > minE/2155524‑2155661 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |