| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,192,465 | C→T | 45.0% | D80N (GAT→AAT) | sspA ← | stringent starvation protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,192,465 | 0 | C | T | 45.0% | 29.6 / 104.2 | 80 | D80N (GAT→AAT) | sspA | stringent starvation protein A |
| Reads supporting (aligned to +/- strand): ref base C (25/19); new base T (16/20); total (41/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.81e-01 | |||||||||||
CAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCT > minE/2192400‑2192533 | cAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGa < 1:1144077/70‑1 (MQ=255)cAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGa < 1:606474/70‑1 (MQ=255)cAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGa > 1:586317/1‑70 (MQ=255) gACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGatat > 1:3428655/1‑71 (MQ=255) aCGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGatat > 1:392337/1‑70 (MQ=255) aCGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATAtt > 1:4136782/1‑71 (MQ=255) cGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGa > 1:114208/1‑66 (MQ=255) cGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTc < 1:1141403/71‑1 (MQ=255) ggCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTc > 1:2256066/1‑70 (MQ=255) cTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCAt < 1:13640/71‑1 (MQ=255) ttCACCGCGAGCTACCTGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATaa > 1:3362463/1‑71 (MQ=255) ttCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATaa > 1:1162037/1‑71 (MQ=255) tCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATaa < 1:2910694/70‑1 (MQ=255) tCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATAat > 1:1694111/1‑71 (MQ=255) tCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATAat > 1:882592/1‑71 (MQ=255) cACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAat < 1:1451303/70‑1 (MQ=255) cACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAat < 1:4002042/70‑1 (MQ=255) aCCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAatga > 1:801608/1‑71 (MQ=255) ccGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATAat < 1:3381376/68‑1 (MQ=255) cgAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATAATGATGCg < 1:1436560/71‑1 (MQ=255) cgAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGc < 1:2359559/70‑1 (MQ=255) gAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATAATGATGCga < 1:2956939/71‑1 (MQ=255) gAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGc > 1:1340503/1‑69 (MQ=255) gCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCa < 1:4062863/58‑1 (MQ=255) gCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAat < 1:1799071/62‑1 (MQ=255) tACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATAat < 1:2108328/60‑1 (MQ=255) aCCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCa > 1:964280/1‑46 (MQ=255) aCCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTc > 1:2997221/1‑53 (MQ=255) aCCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTc > 1:819694/1‑53 (MQ=255) aCCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTc > 1:266820/1‑71 (MQ=255) ccGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATAATGATGCga > 1:178006/1‑65 (MQ=255) ccGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGAtt < 1:2865639/69‑1 (MQ=255) ccGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGAtt < 1:2242744/69‑1 (MQ=255) gggTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATAATGATGCGAGATTccc > 1:3068562/1‑70 (MQ=255) gggTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCAt < 1:396206/53‑1 (MQ=255) gggTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCca < 1:3097378/71‑1 (MQ=255) gggTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCca < 1:2248263/71‑1 (MQ=255) tAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTc < 1:1651973/47‑1 (MQ=255) aaaCAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGata > 1:3749281/1‑43 (MQ=255) aaCAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGa > 1:1347991/1‑40 (MQ=255) aaCAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCcaca > 1:25279/1‑68 (MQ=255) aaCAGGCATCAGTGGAGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCca > 1:4013560/1‑66 (MQ=255) cAGGCATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATAat > 1:759851/1‑49 (MQ=255) cAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGt > 1:3094913/1‑70 (MQ=255) cATCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATAat < 1:2491197/45‑1 (MQ=255) cATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCt < 1:780888/71‑1 (MQ=255) tCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATAATGATGCGAGATTcc < 1:1768536/56‑1 (MQ=255) tCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATAATGATGCGAGATTcc < 1:400107/56‑1 (MQ=255) tCAGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATAATGATGCGAGATTcc < 1:1311709/56‑1 (MQ=255) tCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGAt < 1:2430349/53‑1 (MQ=255) tCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCt < 1:1038824/69‑1 (MQ=255) tCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGAGAGAt < 1:1016619/53‑1 (MQ=255) aGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCAc > 1:591680/1‑70 (MQ=255) aGTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACg < 1:1098936/71‑1 (MQ=255) aGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCgaga > 1:706078/1‑50 (MQ=255) aGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCcaca > 1:3482440/1‑58 (MQ=255) aGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCcaca > 1:3487150/1‑58 (MQ=255) aGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCAc > 1:1626136/1‑70 (MQ=255) aGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACg < 1:3629189/71‑1 (MQ=255) gTGGCGGATGCGGGAAACGCTCATTCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGa < 1:3389584/71‑1 (MQ=255) tGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCcaca < 1:2880387/56‑1 (MQ=255) ggCGGATGCGGGAAACGCTCATTCAGATATTCCATAATGATGCg < 1:1534413/44‑1 (MQ=255) gCGGATGCGGGAAACGCTCATTCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATc > 1:2650220/1‑70 (MQ=255) gATGCGGGAAACGCTCATTCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATccac < 1:3001013/70‑1 (MQ=255) gATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATccacc < 1:4136654/71‑1 (MQ=255) aTGCGGGAAACGCTCATTCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATccacca < 1:2519361/71‑1 (MQ=255) aTGCGGGAAACGCTCATTCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATccac > 1:3782290/1‑69 (MQ=255) tGCGGGAAACGCTCATTCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAg > 1:2525320/1‑71 (MQ=255) tGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAg > 1:122801/1‑71 (MQ=255) gCGGGAAACGCTCATTCAGATATTCCATAatgatg > 1:2643449/1‑35 (MQ=255) gCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAgg > 1:273594/1‑71 (MQ=255) cGGGAAACGCTCATTCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAgg < 1:1781020/70‑1 (MQ=255) gAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAgg > 1:134363/1‑67 (MQ=255) aaCGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGc > 1:3976408/1‑50 (MQ=255) aaCGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCa > 1:2810162/1‑53 (MQ=255) cGCTCATCCAGATATTCCATAATGATGCGAGATTCCca > 1:4076822/1‑38 (MQ=255) gCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACg < 1:2412725/71‑1 (MQ=255) tCATTCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACg > 1:3108157/1‑50 (MQ=255) aTCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGctct > 1:2143606/1‑71 (MQ=255) aTCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGctct < 1:3995160/71‑1 (MQ=255) | CAGACGGCTTTCACCGCGAGCTACCGGGTAAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCT > minE/2192400‑2192533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |