| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,207,505 | C→T | 40.0% | intergenic (‑195/+235) | tldD ← / ← yhdP | predicted peptidase/conserved membrane protein, predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,207,505 | 0 | C | T | 40.0% | 14.5 / 25.3 | 25 | intergenic (‑195/+235) | tldD/yhdP | predicted peptidase/conserved membrane protein, predicted transporter |
| Reads supporting (aligned to +/- strand): ref base C (5/10); new base T (8/2); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01 | |||||||||||
AGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACG > minE/2207439‑2207575 | aGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCTTGa > 1:1614873/1‑70 (MQ=25)aGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCTTGa > 1:4043798/1‑70 (MQ=25) gCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCTTGAt > 1:2862310/1‑69 (MQ=25) gCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATaa < 1:4100797/71‑1 (MQ=34) gCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATaa < 1:3469321/71‑1 (MQ=34) aaCGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGa > 1:1708206/1‑71 (MQ=34) aCGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCTTGATAAGAc > 1:584347/1‑71 (MQ=21) aCGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATaa < 1:2445288/68‑1 (MQ=35) aCGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATaa < 1:3979269/68‑1 (MQ=35) aCGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCAGTTTGTAGGCCTGATaa < 1:1768426/68‑1 (MQ=25) cGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCTTGATAAGACg < 1:2427468/71‑1 (MQ=21) ggCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACg > 1:746819/1‑70 (MQ=35) ggCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACg > 1:1933848/1‑70 (MQ=35) tGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCTTGATAAGACGca > 1:3829605/1‑69 (MQ=25) gTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGGTA‑GTGCGTTTTGTAGGCCTGATAAGACGcg > 1:2815202/1‑66 (MQ=11) tCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCTTGATAAGACGCACAGc > 1:798470/1‑71 (MQ=14) tCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCTTGATAAGACGCACAGc > 1:3687484/1‑71 (MQ=14) tCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGcaca < 1:415437/69‑1 (MQ=31) gTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGTATGCGGCGTa < 1:3862741/71‑1 (MQ=14) gTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGAAGCGGCGTa < 1:3032148/71‑1 (MQ=14) aTGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAAc > 1:1883524/1‑71 (MQ=34) tGTTCCGTTTGTAGGCTTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACg > 1:2789144/1‑71 (MQ=21) cGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCtt < 1:2923824/70‑1 (MQ=37) cGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCtt < 1:2792355/70‑1 (MQ=37) tttGTAGGCTTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACg < 1:1087958/64‑1 (MQ=25) cTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACg > 1:3226532/1‑71 (MQ=34) | AGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACG > minE/2207439‑2207575 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |