| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,258,086 | G→A | 8.4% | D1019D (GAC→GAT) | rpoB ← | RNA polymerase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,258,086 | 0 | G | A | 8.4% | 221.2 / 8.1 | 71 | D1019D (GAC→GAT) | rpoB | RNA polymerase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (33/32); new base A (2/4); total (35/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01 | |||||||||||
CAGATCGTCGCCCTGGGTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTCGTCTGTCAGGCCCAGCTCCAGCC > minE/2258017‑2258153 | cAGATCGTCGCCCTGGGTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGt < 1:3035439/71‑1 (MQ=255)cAGATCGTCGCCCTGGGTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGt < 1:321552/71‑1 (MQ=255) aGATCGTCGCCCTGGGTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGt > 1:1690847/1‑70 (MQ=255) aTCGTCGCCCTGGGTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCa < 1:3297650/70‑1 (MQ=255) aTCGTCGCCCTGGGTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCAt < 1:3065970/71‑1 (MQ=255) tcgtcgCCCTGGGTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATa > 1:1152802/1‑71 (MQ=255) gtcgCCCTGGGTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACt < 1:2274815/71‑1 (MQ=255) gtcgCCCTGGGTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACt < 1:1922313/71‑1 (MQ=255) cgCCCTGGGTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATAGTGc > 1:3100279/1‑71 (MQ=255) gCCCTGGGTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCt < 1:1359600/71‑1 (MQ=255) gCCCTGGGTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCt > 1:3399572/1‑71 (MQ=255) cTGGGTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTAGAACTCGTGTTTCAGTTCGTCATACTGCTcag > 1:3031702/1‑71 (MQ=255) gggTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCa < 1:1737439/59‑1 (MQ=255) ggTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTcagc < 1:354859/69‑1 (MQ=255) ggTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTcagc > 1:3496858/1‑69 (MQ=255) tGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCa < 1:2719764/56‑1 (MQ=255) tGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATAc < 1:911616/59‑1 (MQ=255) aTTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTg > 1:1816217/1‑71 (MQ=255) aTTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTg > 1:1750844/1‑71 (MQ=255) aTTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTg > 1:1449103/1‑71 (MQ=255) ttttGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGt < 1:4096855/71‑1 (MQ=255) ttttGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGt < 1:710741/71‑1 (MQ=255) ttGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTc > 1:2286996/1‑71 (MQ=255) gcgTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCt < 1:1251805/71‑1 (MQ=255) cgTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGtt > 1:2646018/1‑64 (MQ=255) tCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGAttt > 1:3786300/1‑71 (MQ=255) tCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGAttt > 1:834087/1‑71 (MQ=255) tCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGAttt > 1:2730069/1‑71 (MQ=255) tCGCTTCGAGTTTCTTCTCGAACTAGTGTTTCAGTTCGt > 1:2628206/1‑39 (MQ=255) cGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGAtttt > 1:838442/1‑71 (MQ=255) cGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGAttt > 1:695702/1‑70 (MQ=255) cGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGAttt > 1:3546127/1‑70 (MQ=255) gCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTg < 1:3501211/71‑1 (MQ=255) gCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCATCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTg > 1:331770/1‑71 (MQ=255) cTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGt < 1:1077415/71‑1 (MQ=255) cTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGt < 1:368351/71‑1 (MQ=255) cGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGttt < 1:3129692/70‑1 (MQ=255) cGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTtc > 1:1063812/1‑71 (MQ=255) cGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTtc > 1:3035317/1‑71 (MQ=255) aGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTtctc < 1:389079/71‑1 (MQ=255) gTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTtctgt > 1:2063901/1‑69 (MQ=255) gTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTctct < 1:2710819/71‑1 (MQ=255) ttcttcTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTc > 1:2334901/1‑71 (MQ=255) ttcttcTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTc > 1:2329930/1‑71 (MQ=255) ttcttcTCGAACTCGTGTTTCAGTTCATCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTc > 1:2797421/1‑71 (MQ=255) tcttctCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGtt > 1:879234/1‑48 (MQ=255) tcttctCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGtt > 1:1498163/1‑48 (MQ=255) tcttctCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGtt > 1:1509749/1‑48 (MQ=255) ttctCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCt > 1:3734248/1‑52 (MQ=255) ctcGAACTCGTGTTTAAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTCGt < 1:2414323/68‑1 (MQ=255) gAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTtct > 1:2781414/1‑59 (MQ=255) gAACTCGTGTTTCAGTTCATCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTCGTCTGt < 1:2451996/69‑1 (MQ=255) aaCTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTCGTCTGTCAg < 1:3311348/71‑1 (MQ=255) aaCTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTCGTCTGTCAg < 1:1124213/71‑1 (MQ=255) cTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTg < 1:3688239/51‑1 (MQ=255) cTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTtctc > 1:1670435/1‑57 (MQ=255) cTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTtctc > 1:366249/1‑57 (MQ=255) gtgtTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTCGTCTGTCAGGccc < 1:2918352/70‑1 (MQ=255) gtTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGAtttt < 1:1934807/45‑1 (MQ=255) gtTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGAtttt < 1:4054079/45‑1 (MQ=255) gtTTCAGTGCGTCATACTGCTCAGCCAGCTGTTCCAGCTGAtttt < 1:32427/45‑1 (MQ=255) gTTCGTCATACTGCTCCGCCAGCTGTTCCAGCTGAt < 1:3880486/36‑1 (MQ=255) ttCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTCGTCTGTCAGGCCCAGCTccagcc < 1:3850814/71‑1 (MQ=255) ttCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTCGTCTGTCAGGCCCAGCTccagcc < 1:1338804/71‑1 (MQ=255) ttCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTCGTCTGTCAGGCCCAGCTccagcc < 1:2712584/71‑1 (MQ=255) ttCATCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTCGTCTGTCAGGCCCAGCTccagcc < 1:3738504/71‑1 (MQ=255) ttCATCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTCGTCTGTCAGGCCCAGCTccagcc < 1:2057741/71‑1 (MQ=255) ttCATCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTCGTCTGTCAGGCCCAGCTccagcc < 1:3338619/71‑1 (MQ=255) tCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTCGTCTGTCAGGCCCAGCTccagc > 1:343946/1‑69 (MQ=255) cGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGttt > 1:1771984/1‑40 (MQ=255) cGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTCGTCTGt > 1:3214732/1‑52 (MQ=255) gTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTc < 1:714123/45‑1 (MQ=255) | CAGATCGTCGCCCTGGGTGATTTTGCGGCGTTTCGCTTCGAGTTTCTTCTCGAACTCGTGTTTCAGTTCGTCATACTGCTCAGCCAGCTGTTCCAGCTGATTTTGTTTCTCTTCGTCTGTCAGGCCCAGCTCCAGCC > minE/2258017‑2258153 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |