| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,292,617 | A→G | 19.7% | intergenic (+168/‑253) | fsaB → / → gldA | fructose‑6‑phosphate aldolase 2/glycerol dehydrogenase, NAD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,292,617 | 0 | A | G | 19.7% | 175.5 / 37.7 | 81 | intergenic (+168/‑253) | fsaB/gldA | fructose‑6‑phosphate aldolase 2/glycerol dehydrogenase, NAD |
| Reads supporting (aligned to +/- strand): ref base A (40/25); new base G (6/10); total (46/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.76e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTATTAGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGC > minE/2292548‑2292687 | ttattaGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGaaa > 1:3648993/1‑71 (MQ=255) taGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGaaa > 1:683980/1‑67 (MQ=255) taGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACgg > 1:93785/1‑70 (MQ=255) taGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACgg > 1:3727111/1‑70 (MQ=255) cACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACgg < 1:3461354/64‑1 (MQ=255) cTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTAc > 1:2666191/1‑71 (MQ=255) ggCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGaaa > 1:2832008/1‑57 (MQ=255) gCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGt > 1:2661299/1‑71 (MQ=255) gCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGt > 1:2344065/1‑71 (MQ=255) aGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGtt > 1:507774/1‑70 (MQ=255) aGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTc < 1:499337/71‑1 (MQ=255) aGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTc < 1:481374/71‑1 (MQ=255) aGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGt < 1:2465507/64‑1 (MQ=255) ggggCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCGAGGGCGGAAACGGCATTCGTACGGTTCa < 1:24181/71‑1 (MQ=255) ggggCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGaa < 1:2307532/52‑1 (MQ=255) gggCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAg < 1:853203/71‑1 (MQ=255) gCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAgg < 1:2373379/70‑1 (MQ=255) aaTACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTg > 1:3108216/1‑71 (MQ=255) aTACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGCCGGCATTCGTACGGTTCAGGAGCTg > 1:3852332/1‑70 (MQ=255) aTACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTg > 1:618422/1‑70 (MQ=255) tACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCa < 1:393427/71‑1 (MQ=255) tACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACgg > 1:1699033/1‑47 (MQ=255) tACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCa > 1:819400/1‑62 (MQ=255) tACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCa < 1:1216311/71‑1 (MQ=255) cGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCaaa > 1:2216173/1‑71 (MQ=255) cGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAg > 1:3135785/1‑61 (MQ=255) cGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCaaa > 1:3341952/1‑71 (MQ=255) cGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCaa > 1:246607/1‑70 (MQ=255) gTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACgg < 1:2660297/44‑1 (MQ=255) ttGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACg < 1:3718779/71‑1 (MQ=255) tCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGt > 1:821448/1‑71 (MQ=255) tCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGt > 1:889418/1‑71 (MQ=255) ccGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGt > 1:261353/1‑70 (MQ=255) cGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCt > 1:361859/1‑58 (MQ=255) cGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCaaa > 1:3318744/1‑63 (MQ=255) cGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAAc < 1:2395850/64‑1 (MQ=255) cGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAAc < 1:2040330/64‑1 (MQ=255) cGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTa < 1:3295275/71‑1 (MQ=255) tATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTg > 1:1829074/1‑57 (MQ=255) tATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGa < 1:12702/71‑1 (MQ=255) aTGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGa > 1:2293092/1‑70 (MQ=255) tGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaaa > 1:1798882/1‑71 (MQ=255) tGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaaa > 1:3879934/1‑71 (MQ=255) tGTTAACCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaa < 1:2829435/70‑1 (MQ=255) tGTTAACCGCGTAGATGCCCAGGGCGGAACCGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaaa > 1:3496643/1‑71 (MQ=255) tGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaaa > 1:1255266/1‑71 (MQ=255) tGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaa < 1:717588/70‑1 (MQ=255) ttAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATg > 1:3635975/1‑71 (MQ=255) ttAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATg > 1:1581767/1‑71 (MQ=255) tAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATg > 1:2429958/1‑70 (MQ=255) tAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATg > 1:3764414/1‑70 (MQ=255) tAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATg > 1:3612614/1‑70 (MQ=255) aaCCGCGTAGATGCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCa > 1:1458257/1‑71 (MQ=255) aaCCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCa > 1:1634653/1‑71 (MQ=255) aaCCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCa > 1:2673637/1‑71 (MQ=255) aCCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCa < 1:836508/70‑1 (MQ=255) aCCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCa < 1:1461869/70‑1 (MQ=255) aCCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCAc > 1:2969812/1‑71 (MQ=255) cgcgTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCt > 1:345261/1‑47 (MQ=255) gTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAgg < 1:2378310/40‑1 (MQ=255) gTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGtt > 1:356844/1‑56 (MQ=255) gTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGtt > 1:2620806/1‑56 (MQ=255) aTGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGa > 1:1151709/1‑70 (MQ=255) tGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGa > 1:79087/1‑69 (MQ=255) gCCCAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCa < 1:4044879/41‑1 (MQ=255) gCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaaa > 1:3647068/1‑55 (MQ=255) gCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAg > 1:160586/1‑71 (MQ=255) gCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAg > 1:3518577/1‑71 (MQ=255) cccAGGGCGGAGACGTCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAg < 1:2300967/70‑1 (MQ=255) cccAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAg < 1:3080600/70‑1 (MQ=255) cccAGGGCGGAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAg < 1:1068591/70‑1 (MQ=255) cccAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATg < 1:470855/56‑1 (MQ=255) cAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGGGCCAGAAAGCa < 1:2401974/70‑1 (MQ=255) cAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCAt < 1:3347259/71‑1 (MQ=255) gggCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATgg < 1:217435/71‑1 (MQ=255) gggCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATgg > 1:395663/1‑71 (MQ=255) ggCGGAAACGGCATTCGTACGGTTCAGGAGCTGCa < 1:3173565/35‑1 (MQ=255) ggCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGt < 1:3288979/71‑1 (MQ=255) ggCGGAAACGGCATTCGTACCGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGt < 1:4009098/71‑1 (MQ=255) gCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGa < 1:2317586/46‑1 (MQ=255) cGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGaaa > 1:2592983/1‑47 (MQ=255) ggAGACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGa < 1:4073038/44‑1 (MQ=255) gAAACTGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGa < 1:1060699/43‑1 (MQ=255) gAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGa < 1:1908272/43‑1 (MQ=255) gAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCACAAAGCATGGTGCTg > 1:606021/1‑71 (MQ=255) aaCGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGc < 1:1279549/71‑1 (MQ=255) aaCGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCCCCAGAAAGCATGGTGCTGGc < 1:917869/71‑1 (MQ=255) | TTATTAGCCGCACTGGCAGGGGCAAAATACGTTGCTCCGTATGTTAACCGCGTAGATGCCCAGGGCGGAAACGGCATTCGTACGGTTCAGGAGCTGCAAACGCTGTTAGAAATGCACGCGCCAGAAAGCATGGTGCTGGC > minE/2292548‑2292687 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |