| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 310,815 | G→A | 5.8% | D686D (GAC→GAT) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 310,815 | 0 | G | A | 5.8% | 226.7 / 2.9 | 69 | D686D (GAC→GAT) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (36/29); new base A (2/2); total (38/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
GTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAA > minE/310746‑310883 | gTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGt > 1:453074/1‑71 (MQ=255) cTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCaa > 1:1298517/1‑71 (MQ=255) tCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt > 1:1500400/1‑70 (MQ=255) tCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTCACCAAGGCCAGCCTGGTCAAt > 1:1631682/1‑70 (MQ=255) cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt > 1:1303049/1‑69 (MQ=255) gctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCa < 1:1218891/70‑1 (MQ=255) ctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCa > 1:1615559/1‑69 (MQ=255) gctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt > 1:2075878/1‑70 (MQ=255) ctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTc < 1:377604/61‑1 (MQ=255) ctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca < 1:498104/71‑1 (MQ=255) ctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca < 1:3699435/71‑1 (MQ=255) ctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtc > 1:4019568/1‑70 (MQ=255) tCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca > 1:2032543/1‑69 (MQ=255) gcaagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagt > 1:3668354/1‑70 (MQ=255) gcaagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCAAGCCTGGTCAATCAGCtcaaagt > 1:2797037/1‑70 (MQ=255) ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaa > 1:476855/2‑68 (MQ=255) ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaa > 1:1111326/2‑68 (MQ=255) caagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagt < 1:3593032/69‑1 (MQ=255) aagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcga < 1:3619062/71‑3 (MQ=255) aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca > 1:2451158/1‑70 (MQ=255) gcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCTATTAACtcaaa < 1:2812089/64‑1 (MQ=255) gcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag > 1:3793891/1‑71 (MQ=255) caaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGc > 1:28976/1‑71 (MQ=255) aaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc > 1:976190/1‑71 (MQ=255) cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCa < 1:1189699/49‑1 (MQ=255) cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:3286078/69‑1 (MQ=255) tGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGt > 1:4017314/1‑71 (MQ=255) ggTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATc > 1:823528/1‑50 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGt < 1:2653576/69‑1 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg < 1:1415522/71‑1 (MQ=255) ttACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCATCCTGGTCAATCAGCTCAAAGTCAAAGCGGGTTg < 1:1118833/70‑1 (MQ=255) ttACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg > 1:817641/1‑70 (MQ=255) cgcgCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg < 1:263900/67‑1 (MQ=255) cgcgCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAg < 1:2273834/70‑1 (MQ=255) cgcgCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAg < 1:2561521/70‑1 (MQ=255) aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGtt > 1:985914/1‑71 (MQ=255) gTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTc > 1:1171458/1‑71 (MQ=255) tgaGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTc > 1:2442801/3‑70 (MQ=255) tCAGTTTTTCGTGACCAAGGCCGGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:2581810/52‑1 (MQ=255) tCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTcc < 1:2049929/71‑1 (MQ=255) aGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCa < 1:3308177/70‑1 (MQ=255) aGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCAc > 1:3860641/1‑71 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa < 1:930832/38‑1 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCg < 1:2477937/49‑1 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCa > 1:24111/1‑68 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCAc > 1:2252873/1‑69 (MQ=255) ttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGAt > 1:2747150/1‑71 (MQ=255) tttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCgg > 1:2260991/1‑48 (MQ=255) tttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAg > 1:424045/1‑54 (MQ=255) tttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGAtt > 1:3760848/1‑71 (MQ=255) tttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGAtt > 1:1089315/1‑71 (MQ=255) tttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGAt < 1:1618945/70‑1 (MQ=255) tGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGc < 1:3858891/40‑1 (MQ=255) tGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTc < 1:3734477/59‑1 (MQ=255) aCCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCggg > 1:657521/1‑69 (MQ=255) aCCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGc < 1:2443875/70‑1 (MQ=255) cAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCg > 1:1704989/1‑38 (MQ=255) cAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTc < 1:4000403/55‑1 (MQ=255) aaGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGc < 1:3362337/42‑1 (MQ=255) aaGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGc < 1:129647/42‑1 (MQ=255) aGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGt > 1:2075322/1‑70 (MQ=255) ggCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGtt > 1:587665/1‑70 (MQ=255) ggCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTa < 1:2447681/71‑1 (MQ=255) ggCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTa < 1:884100/71‑1 (MQ=255) gCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCgg > 1:2974904/1‑35 (MQ=255) gCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTcc < 1:1814448/52‑1 (MQ=255) ccTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGc > 1:703462/1‑70 (MQ=255) ccTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCg < 1:2063356/71‑1 (MQ=255) tGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGa > 1:3218036/1‑70 (MQ=255) ggTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGaa > 1:2977729/1‑70 (MQ=255) gTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCa < 1:3636614/56‑1 (MQ=255) | GTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAA > minE/310746‑310883 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |