| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,294,050 | A→C | 18.6% | intergenic (+77/‑198) | gldA → / → yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,294,050 | 0 | A | C | 18.6% | 121.6 / 38.0 | 70 | intergenic (+77/‑198) | gldA/yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (26/31); new base C (5/8); total (31/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
CCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCAC > minE/2293988‑2294118 | ccGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGc < 1:295183/71‑1 (MQ=255) cGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCa < 1:1818989/71‑1 (MQ=255) cTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAata < 1:2046117/71‑1 (MQ=255) cGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGa < 1:3960317/71‑1 (MQ=255) gggAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCaa < 1:4085014/62‑1 (MQ=255) aGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAttt < 1:601405/71‑1 (MQ=255) aGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAttt < 1:4037149/71‑1 (MQ=255) tGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAatata > 1:1004926/1‑59 (MQ=255) tGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGt > 1:2806764/1‑71 (MQ=255) tGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGt > 1:2664707/1‑71 (MQ=255) gAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAAtc < 1:2129346/47‑1 (MQ=255) accGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCaa < 1:1663005/51‑1 (MQ=14) aaCGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAatata < 1:763694/57‑1 (MQ=255) aaCGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAatata < 1:649066/57‑1 (MQ=255) cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtt < 1:3655105/71‑1 (MQ=255) gCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCttt > 1:2404164/1‑71 (MQ=255) cAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCtttt > 1:944550/1‑71 (MQ=255) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:2957537/1‑70 (MQ=255) aTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:3233528/1‑68 (MQ=255) aTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAg > 1:1762764/1‑71 (MQ=255) ttGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAg < 1:1090479/70‑1 (MQ=255) atGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCt < 1:1500061/62‑1 (MQ=255) cTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCttt < 1:3973634/61‑1 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCt < 1:2019713/58‑1 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAttt < 1:3700046/51‑1 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAttt < 1:2636353/51‑1 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAatata < 1:3025520/42‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCttt > 1:2503167/1‑58 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGAt < 1:714379/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt < 1:3630694/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt < 1:1186774/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt < 1:2068245/70‑1 (MQ=255) tGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTa > 1:3923444/1‑61 (MQ=255) tGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTCTTGTa > 1:3955572/1‑61 (MQ=255) gcgcTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAg > 1:1506268/1‑71 (MQ=255) cgcTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCg > 1:3083047/1‑49 (MQ=255) cgcTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTa > 1:1513172/1‑59 (MQ=255) cgcTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAg < 1:2085056/70‑1 (MQ=255) cgcTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAg < 1:3893042/70‑1 (MQ=255) gcTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGc < 1:3695798/71‑1 (MQ=255) cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCg > 1:1128869/1‑71 (MQ=255) tACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGc < 1:3171633/49‑1 (MQ=255) aCGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGcag < 1:3758293/60‑3 (MQ=255) aCGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt > 1:2967751/1‑71 (MQ=255) aCGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt > 1:634413/1‑71 (MQ=255) aCGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt > 1:2012027/1‑71 (MQ=255) cGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:2181348/70‑1 (MQ=255) cGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAACGCGtt < 1:3806914/70‑1 (MQ=255) cTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTg < 1:3015579/50‑1 (MQ=255) cTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCAc > 1:3332386/1‑71 (MQ=255) tATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTa > 1:2796250/1‑50 (MQ=255) aTCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTcc > 1:1556165/1‑71 (MQ=255) tCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCg < 1:3480846/71‑1 (MQ=14) cAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCa > 1:2325473/1‑65 (MQ=255) cAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTa > 1:657776/1‑47 (MQ=255) ggCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCAt < 1:2421985/71‑1 (MQ=255) cTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATa > 1:2168502/1‑51 (MQ=255) cTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCg > 1:3070385/1‑71 (MQ=255) tACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt < 1:192892/49‑1 (MQ=255) tACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAgg < 1:3873079/53‑1 (MQ=255) tACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAgg < 1:2592740/53‑1 (MQ=255) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:567249/1‑37 (MQ=255) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa < 1:1927264/49‑1 (MQ=14) cGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt < 1:191330/47‑1 (MQ=255) cGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGc > 1:1731883/1‑52 (MQ=255) cGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCgg < 1:2956246/69‑1 (MQ=255) cGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCa > 1:3182553/1‑71 (MQ=255) cGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCa > 1:1297534/1‑71 (MQ=255) cAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTa > 1:655763/1‑37 (MQ=255) cAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCAc > 1:1030857/1‑70 (MQ=255) | CCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCAC > minE/2293988‑2294118 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |