| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,294,089 | C→A | 7.1% | intergenic (+116/‑159) | gldA → / → yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,294,089 | 0 | C | A | 7.1% | 270.4 / 3.6 | 82 | intergenic (+116/‑159) | gldA/yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (35/41); new base A (3/3); total (38/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
ATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAATT > minE/2294024‑2294159 | aTGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGAt < 1:714379/70‑1 (MQ=255)aTGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt < 1:2068245/70‑1 (MQ=255)aTGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt < 1:1186774/70‑1 (MQ=255)aTGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt < 1:3630694/70‑1 (MQ=255) gcgcTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAg > 1:1506268/1‑71 (MQ=255) cgcTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAg < 1:2085056/70‑1 (MQ=255) cgcTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAg < 1:3893042/70‑1 (MQ=255) gcTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGc < 1:3695798/71‑1 (MQ=255) cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCg > 1:1128869/1‑71 (MQ=255) aCGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt > 1:2012027/1‑71 (MQ=255) aCGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt > 1:2967751/1‑71 (MQ=255) aCGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt > 1:634413/1‑71 (MQ=255) cGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:2181348/70‑1 (MQ=255) cGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAACGCGtt < 1:3806914/70‑1 (MQ=255) cTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCAc > 1:3332386/1‑71 (MQ=255) aTCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTcc > 1:1556165/1‑71 (MQ=255) tCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCg < 1:3480846/71‑1 (MQ=14) cAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCa > 1:2325473/1‑65 (MQ=255) ggCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCAt < 1:2421985/71‑1 (MQ=255) cTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATa > 1:2168502/1‑51 (MQ=255) cTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCg > 1:3070385/1‑71 (MQ=255) tACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt < 1:192892/49‑1 (MQ=255) tACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAgg < 1:2592740/53‑1 (MQ=255) tACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAgg < 1:3873079/53‑1 (MQ=255) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa < 1:1927264/49‑1 (MQ=14) cGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt < 1:191330/47‑1 (MQ=255) cGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGc > 1:1731883/1‑52 (MQ=255) cGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCgg < 1:2956246/69‑1 (MQ=255) cGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCa > 1:1297534/1‑71 (MQ=255) cGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCa > 1:3182553/1‑71 (MQ=255) cAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCAc > 1:1030857/1‑70 (MQ=255) aTCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCAct > 1:1734662/1‑69 (MQ=255) aTCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCAct > 1:3953735/1‑69 (MQ=255) tctcTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAg < 1:615271/45‑1 (MQ=38) tctcTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:340762/50‑1 (MQ=38) tctcTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:1636459/50‑1 (MQ=38) tctcTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACt > 1:437055/1‑54 (MQ=38) tctGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt > 1:1406817/1‑48 (MQ=38) tctGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt > 1:1450019/1‑48 (MQ=38) tctGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCtgtg > 1:3630360/1‑71 (MQ=255) ctGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGc > 1:2569559/1‑71 (MQ=255) tGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCtgt < 1:3972263/68‑1 (MQ=255) tGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGc > 1:1372613/1‑70 (MQ=255) cAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATc < 1:1961988/55‑1 (MQ=255) cAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGc < 1:507768/68‑1 (MQ=255) cAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAg > 1:1854842/1‑71 (MQ=255) cAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAg > 1:2543633/1‑71 (MQ=255) cAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAg < 1:1646778/71‑1 (MQ=255) aaTATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:544843/43‑1 (MQ=38) atatatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTa < 1:3243889/71‑1 (MQ=255) tatatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATcc < 1:411789/53‑1 (MQ=255) tatatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTaa > 1:2767882/1‑71 (MQ=255) tatatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTaa > 1:1729253/1‑71 (MQ=255) tatatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTa < 1:2140431/70‑1 (MQ=255) atatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCa > 1:2853772/1‑66 (MQ=255) atatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTaa > 1:1520036/1‑70 (MQ=255) tatTGAATTTGCGTGCTTTTGTAGGCCGGATAAgg > 1:1334204/1‑35 (MQ=40) tatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt > 1:2710608/1‑39 (MQ=40) tatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:1772589/39‑1 (MQ=40) tatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:3565644/39‑1 (MQ=40) tatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:1253613/39‑1 (MQ=40) tatTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCAt > 1:4006104/1‑49 (MQ=255) tGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCa > 1:2142197/1‑52 (MQ=255) gAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCtg > 1:3937235/1‑56 (MQ=255) gAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTa > 1:768728/1‑64 (MQ=255) gAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAAt < 1:1060773/66‑1 (MQ=255) gAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGa < 1:85581/70‑1 (MQ=255) gAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGa < 1:121702/70‑1 (MQ=255) gAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGa < 1:1347440/70‑1 (MQ=255) gAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGa > 1:3751262/1‑70 (MQ=255) gAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGa < 1:1609747/70‑1 (MQ=255) aaTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAAt < 1:2357703/65‑1 (MQ=255) aTTTGAGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTATGTGCCAGTAATc > 1:2518646/1‑65 (MQ=255) tttGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCa < 1:1505757/41‑1 (MQ=38) tttGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACt < 1:482559/71‑1 (MQ=255) ttGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTa < 1:2681901/60‑1 (MQ=255) tGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATc > 1:595729/1‑41 (MQ=38) ttGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACgttgtt > 1:2476372/1‑71 (MQ=255) ttGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACgttg < 1:685027/69‑1 (MQ=255) tGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACgttgtt < 1:657045/70‑1 (MQ=255) gCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGaa < 1:2241761/71‑1 (MQ=255) gCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGaa < 1:818472/71‑1 (MQ=255) ccGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACg < 1:1414828/59‑1 (MQ=255) ccGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAATAATCTGAAACTCCCACg < 1:521625/59‑1 (MQ=255) cGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAAtt > 1:1497371/1‑71 (MQ=255) cGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAAtt > 1:3681890/1‑71 (MQ=255) | ATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCACTCTGTGCCAGTAATCTGAAACTCCCACGTTGTTCAGGAATT > minE/2294024‑2294159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |