| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,331,252 | A→C | 18.1% | intergenic (‑157/+273) | sodA ← / ← fdhD | superoxide dismutase, Mn/formate dehydrogenase formation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,331,252 | 0 | A | C | 18.1% | 133.3 / 43.4 | 83 | intergenic (‑157/+273) | sodA/fdhD | superoxide dismutase, Mn/formate dehydrogenase formation protein |
| Reads supporting (aligned to +/- strand): ref base A (40/28); new base C (6/9); total (46/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01 | |||||||||||
GATTATCAATGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTAATCGACAGCAC > minE/2331182‑2331320 | gATTATCAATGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCaa > 1:1359930/1‑71 (MQ=255) aTTATCAATGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCaa < 1:1146457/70‑1 (MQ=255) ttATCAATGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAAcc < 1:1637279/71‑1 (MQ=255) tATCAATGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCa > 1:1154541/1‑71 (MQ=255) aTCAATGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCa < 1:3797395/70‑1 (MQ=255) cAATGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAAc > 1:2515366/1‑66 (MQ=255) cAATGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACt > 1:3243860/1‑70 (MQ=255) aaTGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCa > 1:3350491/1‑67 (MQ=255) aaTGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCa > 1:3236261/1‑67 (MQ=255) aaTGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACtt < 1:2836477/70‑1 (MQ=255) aaTGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACtt < 1:84867/70‑1 (MQ=255) aaTGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACtt < 1:3062736/70‑1 (MQ=255) aaTGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACtt < 1:1780546/70‑1 (MQ=255) tGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCa > 1:3608548/1‑65 (MQ=255) tGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAAt > 1:3225817/1‑71 (MQ=255) ccGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATg < 1:1563312/70‑1 (MQ=255) ccGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGc > 1:2728994/1‑71 (MQ=255) cGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGcc < 1:3620749/71‑1 (MQ=255) cGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGcc > 1:432873/1‑71 (MQ=255) tACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGt > 1:1083941/1‑71 (MQ=255) tACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGt > 1:1240776/1‑71 (MQ=255) aCTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGt < 1:407390/70‑1 (MQ=255) cTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTg < 1:3097943/70‑1 (MQ=255) cTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTg < 1:938802/70‑1 (MQ=255) cTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGa > 1:2820442/1‑71 (MQ=255) ttttCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACtt < 1:3049609/60‑1 (MQ=255) ttttCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACt < 1:475927/59‑1 (MQ=255) ttCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCaa > 1:3452070/1‑52 (MQ=255) ttCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCa > 1:3544020/1‑55 (MQ=255) ttCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCa > 1:563977/1‑55 (MQ=255) ttCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGAt > 1:2529322/1‑69 (MQ=255) cGTAAGGGTATGGTTTTGCAGGAAAATGCCCTAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGc < 1:3141048/70‑1 (MQ=255) gTAAGGGTATGGTTTTGCATGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTg < 1:3010853/68‑1 (MQ=255) gTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCAGTGATTg < 1:3434381/68‑1 (MQ=255) aaGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAg > 1:1109911/1‑70 (MQ=255) aGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGt > 1:1317595/1‑70 (MQ=255) ggTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTaaa < 1:1317703/71‑1 (MQ=255) gTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAAt > 1:4009271/1‑71 (MQ=255) gTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCAACTTAATGCCGTGATTGCCAGTAAAt > 1:3491721/1‑71 (MQ=255) tATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATc < 1:3321874/71‑1 (MQ=255) aTGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGt < 1:3715981/55‑1 (MQ=255) tGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACtt < 1:204347/46‑1 (MQ=255) ggTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGa > 1:1164973/1‑70 (MQ=255) gTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACtt < 1:2465117/44‑1 (MQ=255) gTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCACCCACTTaa > 1:2362358/1‑46 (MQ=255) gTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGAtt > 1:3430511/1‑56 (MQ=255) gTTTTGCAGGAAAATGCCCGAGAAGTGAAGCAAATCACCCACtt < 1:741845/44‑1 (MQ=38) ttGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACt < 1:548003/40‑1 (MQ=255) ttGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACt < 1:724235/40‑1 (MQ=255) ttGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACt < 1:1754542/40‑1 (MQ=255) ttGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTaa > 1:943259/1‑43 (MQ=255) cAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTaaa < 1:2453899/59‑1 (MQ=255) ggAAAATGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAACggcggc < 1:2588241/71‑1 (MQ=255) ggAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATc > 1:767844/1‑59 (MQ=255) ggAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAAcggcgg > 1:564955/1‑70 (MQ=255) gAAAATGCTCGAGATGTGAAGCAAATCACCCACTTAATGCCGTg < 1:1852711/44‑1 (MQ=37) gAAAATGCCCGAGATGTGAAGCAAATCAACCACTTaa > 1:3527892/1‑37 (MQ=255) gAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGt < 1:2185982/43‑1 (MQ=255) gAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGAt > 1:1121254/1‑46 (MQ=255) gAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACaa > 1:3235466/1‑63 (MQ=255) gAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACggcggc > 1:4086578/1‑70 (MQ=255) aaaaTGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCaa > 1:2351363/1‑71 (MQ=255) aaaTGCCCGAGATGTGAAGCAAATCAACCACTTaa > 1:1074531/1‑35 (MQ=255) aaaTGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACaa > 1:525720/1‑61 (MQ=255) aaTGCCCGAGATGTGAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCaa > 1:3126002/1‑69 (MQ=255) aaTGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACa < 1:598756/71‑1 (MQ=255) tGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGAt > 1:3609962/1‑41 (MQ=255) tGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGAt > 1:1056999/1‑41 (MQ=255) tGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAgg > 1:851087/1‑71 (MQ=255) tGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAgg > 1:2735033/1‑71 (MQ=255) tgAAGCAAATCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTaa > 1:1798860/1‑71 (MQ=255) tgAAGCAAATCAACCACTTAATGCCGTGATTGCCa > 1:782517/1‑35 (MQ=255) aaGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTAATc < 1:976579/71‑1 (MQ=255) aaGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTAATc < 1:411928/71‑1 (MQ=255) aGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCaa > 1:1358328/1‑53 (MQ=255) gCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGa > 1:811371/1‑40 (MQ=255) gCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTAATCg > 1:1738474/1‑70 (MQ=255) gCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTAATCGa < 1:3510469/71‑1 (MQ=255) gCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTAATCGa > 1:3282303/1‑71 (MQ=255) cAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCCGCAACAGGCGAAAGGTTAATCGa > 1:517104/1‑70 (MQ=255) aaTCACCCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTAATCGACAg < 1:156011/71‑1 (MQ=255) aTCACCCACTTAATGCCGTGATTGCCAGTAAATCGAc < 1:4078321/37‑1 (MQ=255) tCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTAATCGACAGCa > 1:2156980/1‑71 (MQ=255) cAACCACTTAATGCCGTGATTGCCAGTAAATCGACAAcggc < 1:2878447/41‑1 (MQ=255) cAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACa < 1:593823/48‑1 (MQ=255) cAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTAATCGACAGCAc > 1:1313065/1‑71 (MQ=255) aaCCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTAATCGACAGCAc < 1:2697115/70‑1 (MQ=255) | GATTATCAATGCCGTACTTTTCGTAAGGGTATGGTTTTGCAGGAAAATGCCCGAGATGTGAAGCAAATCAACCACTTAATGCCGTGATTGCCAGTAAATCGACAACGGCGGCAACAGGCGAAAGGTTAATCGACAGCAC > minE/2331182‑2331320 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |