| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,402,822 | T→C | 12.6% | E70G (GAG→GGG) | yigA ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,402,822 | 0 | T | C | 12.6% | 211.5 / 19.9 | 79 | E70G (GAG→GGG) | yigA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (39/30); new base C (7/3); total (46/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGTAGAGTAGGCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACGGTGCCGCGTA > minE/2402756‑2402891 | aGGTAGAGTAGGCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTc < 1:2112100/71‑1 (MQ=255)aGGTAGAGTAGGCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTc < 1:2107303/71‑1 (MQ=255) agagTAGGCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAg > 1:2459402/1‑70 (MQ=255) gagTAGGCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGaa > 1:104652/1‑71 (MQ=255) gagTAGGCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGaa < 1:2816923/71‑1 (MQ=255) tAGGCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTcc < 1:3944375/64‑1 (MQ=255) tAGGCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCCCTTCCAGAACAt > 1:1088833/1‑71 (MQ=255) tAGGCGATAAAACAGGCATTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACAt < 1:2713404/71‑1 (MQ=255) ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAg > 1:3781648/1‑64 (MQ=255) ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAg > 1:2152182/1‑64 (MQ=255) ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATg > 1:909589/1‑70 (MQ=255) ggCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATg > 1:674062/1‑70 (MQ=255) aaaaCAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGa > 1:841467/1‑71 (MQ=255) aaaaCAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGa > 1:150389/1‑71 (MQ=255) aaaaCAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGa > 1:2706003/1‑71 (MQ=255) aaaaCAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGa > 1:2515528/1‑71 (MQ=255) aaaCAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAatat > 1:751138/1‑68 (MQ=255) aCAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTa > 1:2099957/1‑71 (MQ=255) aGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTAc < 1:992500/70‑1 (MQ=255) aGGCCTTCGTTGGCGAGAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGaa < 1:3182936/55‑1 (MQ=255) ttCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTcc < 1:1372002/46‑1 (MQ=255) tCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGaa > 1:2382646/1‑49 (MQ=255) cGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTtct > 1:1907655/1‑39 (MQ=255) cGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCgg > 1:1279339/1‑71 (MQ=255) cGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCgg > 1:528451/1‑71 (MQ=255) gTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGaa > 1:3814154/1‑53 (MQ=255) ttGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGaa < 1:3163529/52‑1 (MQ=255) ttGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGaa < 1:2658030/52‑1 (MQ=255) ttGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGc < 1:2610056/71‑1 (MQ=255) cGATAGCCTGTTCCATCAACAGCGCCATGTTCCCGTCCAGAACATGAATATGATTACGTGCGCGGGCCAtg > 1:2754967/1‑71 (MQ=255) gATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGaa < 1:3104350/47‑1 (MQ=255) aTAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTAc < 1:1501474/55‑1 (MQ=255) tAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCAtg > 1:3641553/1‑68 (MQ=255) tAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGc > 1:3170504/1‑71 (MQ=255) tAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGc > 1:689001/1‑71 (MQ=255) gCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGcc < 1:3661542/70‑1 (MQ=255) gCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCa > 1:1826461/1‑71 (MQ=255) ccTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGaa < 1:666511/42‑1 (MQ=255) tGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCAt > 1:2530941/1‑62 (MQ=255) gTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAat < 1:338143/40‑1 (MQ=255) ttCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTc < 1:3211451/69‑1 (MQ=255) tCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAc > 1:216749/1‑71 (MQ=255) ccATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTa > 1:237016/1‑44 (MQ=255) ccATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGcc > 1:529877/1‑56 (MQ=255) ccATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCa > 1:61628/1‑57 (MQ=255) ccATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAc < 1:463961/70‑1 (MQ=255) cATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTa > 1:1920456/1‑43 (MQ=255) cATCAACAGCGCCATGTTCCCTTCCAGAACATGAatat < 1:3703138/38‑1 (MQ=255) aTCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCaa > 1:2794849/1‑71 (MQ=255) cAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCa > 1:1151178/1‑53 (MQ=255) cAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAcc > 1:900403/1‑67 (MQ=255) cAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAAc > 1:547379/1‑70 (MQ=255) aaCAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACg > 1:1589713/1‑70 (MQ=255) aaCAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACg > 1:3622210/1‑70 (MQ=255) aaCAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACg > 1:97243/1‑70 (MQ=255) aaCAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACg > 1:2405791/1‑70 (MQ=255) aaCAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGa < 1:3858947/71‑1 (MQ=255) aaCAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCAACTCGACCAACGa < 1:713839/71‑1 (MQ=255) aaCAGCGCCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCa > 1:45451/1‑52 (MQ=255) aCAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCa < 1:3670497/66‑1 (MQ=255) aCAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGa < 1:3563775/70‑1 (MQ=255) aCAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGa < 1:1978583/70‑1 (MQ=255) aCAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGa < 1:1766496/70‑1 (MQ=255) aGCGCCATGTTCTCTTCCAGAACACGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAAc < 1:571485/71‑1 (MQ=255) gcgcCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAAc < 1:1028773/70‑1 (MQ=255) cgcCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGAc > 1:1735441/1‑60 (MQ=255) cgcCATGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACgg < 1:1638341/71‑1 (MQ=255) ctaTGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACGGTg > 1:2986979/3‑71 (MQ=255) cATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACGGTGc < 1:469616/71‑1 (MQ=255) aTGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGc < 1:1741220/41‑1 (MQ=255) aTGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGc < 1:2478827/41‑1 (MQ=255) tGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACGGTGcc > 1:3233186/1‑70 (MQ=255) tGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACGGTGCcg > 1:768155/1‑71 (MQ=255) tGTTCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGa < 1:563477/61‑1 (MQ=255) gTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGcc < 1:1476881/40‑1 (MQ=255) ttCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACGGTGCcgcg > 1:2437768/1‑71 (MQ=255) ttCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACGGTGCcgcg > 1:3551525/1‑71 (MQ=255) ttCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACGGTGCcgcg > 1:816967/1‑71 (MQ=255) ttCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACGGTGCcgcg > 1:1471857/1‑71 (MQ=255) ttCCCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACGGTGCcgc > 1:579846/1‑70 (MQ=255) tctcTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACGGt < 1:2758995/64‑1 (MQ=255) tctcTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACGGTGCcgcg > 1:1228258/1‑70 (MQ=255) tctcTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACGGTGCcgcg > 1:2728337/1‑70 (MQ=255) ctctTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGaa < 1:279077/58‑1 (MQ=255) ctctTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACGGTGCCGCGt < 1:474137/70‑1 (MQ=255) ctctTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACGGTGCCGCGTa > 1:1547415/1‑71 (MQ=255) | AGGTAGAGTAGGCGATAAAACAGGCCTTCGTTGGCGATAGCCTGTTCCATCAACAGCGCCATGTTCTCTTCCAGAACATGAATATGATTACGTGCGCGGGCCATGTGCCACTCGACCAACGAAACGGTGCCGCGTA > minE/2402756‑2402891 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |